Abstract: Safe and effective live vaccines against bacteria infecting aquatic animals were created through the induction of novobiocin-resistance in liquid culture and novobiocin- and rifampicin-resistance in liquid culture.

Abstract: Safe and effective live vaccines against bacteria infecting aquatic animals were created through the induction of novobiocin-resistance in liquid culture and novobiocin- and rifampicin-resistance in liquid culture.

Abstract: Safe and effective live vaccines against Aeromonas hydrophila of fish were created through the induction of rifampicin resistance in native Aeromonas hydrophila isolates; these including rifampicin-resistant mutants NRRL-B-50040 and NRRL-B-50041. Single immersion exposure of fish stimulated acquired immunity against virulent Aeromonas hydrophila infection.

Abstract: Safe and effective live vaccines against Aeromonas hydrophila of fish were created through the induction of rifampicin resistance in native Aeromonas hydrophila isolates; these including rifampicin-resistant mutants NRRL-B-50040 and NRRL-B-50041. Single immersion exposure of fish stimulated acquired immunity against virulent Aeromonas hydrophila infection.

Abstract: A composition prepared from intact killed cells of isolated ?-hemolytic Streptococcus agalactiae and a concentrated extract of a culture of ?-hemolytic Streptococcus agalactiae, is effective for the protection of fish against infection by the same and other virulent strains Streptococcus agalactiae.

Abstract: A safe and effective live vaccine against Edwardsiella tarda in fish was created through the induction of rifampicin resistance in a native Edwardsiella tarda isolate. Single immersion exposure or injection of fish stimulated acquired immunity against virulent E. tarda infection.

Abstract: Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: A safe and effective live vaccine against Flavobacterium columnare of fish was created through the induction of an adhesion deficient isolate of Flavobacterium columnare using a ?-lactam antibiotic (ampicillin). Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

Abstract: Safe and effective mono and polyvalent vaccines against Streptococcus iniae may be prepared from formalin-killed cells and concentrated extracellular products of Streptococcus iniae which include one or more of deposited strains NRRL B-30238 and NRRL B-30242. Intraperitoneal and intramuscular vaccination of tilapia show acquisition of effective immunity against homologous and heterologous isolates of S. iniae.

Abstract: Methods for the safe and effective live in ovo vaccination of eyed eggs of catfish against enteric septicemia of catfish (ESC) were created through the use of rifampicin resistant native E. ictaluri isolates; these including rifampicin-resistant strain ATCC 202058 of Edwardsiella ictaluri originally isolated from the walking catfish Clarius batrachus from Thailand. Single immersion exposure of eyed eggs of catfish stimulated strong acquired immunity against many isolates of E. ictaluri without the need for booster immunization.

Abstract: Safe and effective live vaccines against enteric septicemia of catfish (ESC) were created through the induction of rifampicin resistance in native E. ictaluri isolates; these including rifampicin-resistant strain (RE-33) of Edwardsiella ictaluri originally isolated from the walking catfish Clarius batrachus from Thailand. Single immersion exposure of catfish fry stimulated strong acquired immunity against many isolates of E. ictaluri without the need for booster immunization.

Abstract: A hybridoma cell line produces an antibody with reactivity directed specifically to immunodominant antigens from the catfish pathogen Edwardsiella ictaluri, the antigens having molecular weights of about 14,000, 39,000, 48,000, 60,000, 66,000, 83,000, and 93,000 daltons. This antibody is specific for proteins of E. ictaluri but not for other catfish pathogens. It is therefore useful for the positive identification of E. ictaluri from body fluids and tissue of catfish and for identification and purification of the reactive immunodominant antigens.

Abstract: The instant invention pertains to improvements in processes and apparatus for stimulating, collecting, and optionally modifying, macrophages from the peritoneal cavity of a vertebrate.

Abstract: An apparatus for in vivo stimulation and collection of monocytes from peritoneum of vertebrate is disclosed. The apparatus is implanted between the skin and the peritoneum. This device stimulates the production and growth of monocytes and at the same time attracts the monocytes into the apparatus. The apparatus comprises a container which has a plurality of membrane-covered holes for monocyte entry. There is an inlet and outlet conduit which is attached to opposing sides of the container to allow flushing. The conduits are equipped with a lock tip coupler for connection when the flushing operation is not taking place.

Abstract: Precipitates soluble in aqueous solution and suitable for stimulation and/or transfer of cell-mediated immunity in mammals and other animals is disclosed together with a solvent extraction process for obtaining this desirable product from crude bovine transfer factor. Lymphocytes from lymph nodes and blood of donor cattle are employed in formulating a Bovine Transfer factor by incubation release procedure. The fractionation of Bovine Transfer factor yields three precipitate fractions by the use of organic solvents. A relatively high incidence of transfer with the precipitates yields excellent immunity to certain diseases in animals.