Abstract: This disclosure relates to protease-cleavable cytokine prodrugs. In some embodiments, the prodrugs comprise a targeting sequence. In some embodiments, the prodrugs comprise a pharmacokinetic modulator.

Abstract: This disclosure relates to protease-cleavable IL-2 cytokine prodrugs. In some embodiments, the prodrugs comprise a pharmacokinetic modulator.

Abstract: Provided herein are methods for activating an immune cell in a subject. In some embodiments, the methods comprise passing an immune cell from a subject through an immune modulating chamber comprising a tumor cell, thereby activating the immune cell, and returning the activated immune cell to the subject. In some embodiments, the methods further comprise isolating an immune cell-containing portion of a sample and passing the immune cell-containing portion through the immune modulating chamber. Methods of treating cancer and methods of inducing an immune response against a tumor are also provided herein.

Abstract: The invention is an apparatus which enables the operation of a dispensing assembly in a container containing fluid from either an inverted (upside-down), angles, or upright position. The apparatus serves as a fluid intake tube, and is fitted into the dispensing assembly of a container containing a fluid. The apparatus is composed to two generally tubular parts mounted concentrically: an outer tube, and an inner tube. On the outer tube, buoyant ring(s) are located, with confer buoyancy to the outer tube. The outer tube is slightly shorter than the inner tube. When the apparatus is fitted into a fluid-filled container, and said container is held in an inverted or downward-angled position, the perforations located at the end of the inner tube proximal to the dispensing assembly are exposed. The exposed perforations provide for uptake of fluid in the tube into the dispensing assembly of the liquid container.