Patents by Inventor Phillip Samayoa

Phillip Samayoa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240299580
    Abstract: Provided herein are pharmaceutical compositions comprising a lipid nanoparticle (LNP) and a therapeutic nucleic acid (TNA), wherein the LNP comprises an ApoE polypeptide, or a fragment thereof and/or an ApoB polypeptide, or a fragment thereof, linked to the LNP, and at least one pharmaceutically acceptable excipient. The ApoE polypeptide, or the fragment thereof, and/or the ApoB polypeptide, or the fragment thereof, are capable of binding a low-density lipoprotein (LDL) receptor, or LDL receptor family member, advantageously providing LNP compositions that can be directed to any cell or tissue expressing the LDL receptor.
    Type: Application
    Filed: June 7, 2022
    Publication date: September 12, 2024
    Inventors: Michelle LeBlanc, Gregory Feinstein, Randall Newton Toy, Birte Nolting, Nathaniel Silver, Phillip Samayoa, Jon Edward Chatterton, Matthew Manganiello, Douglas A. Rose, Ryan M. Delaney
  • Publication number: 20240277833
    Abstract: The application describes methods and compositions comprising ceDNA vectors useful for the expression of antigens and immunogenic peptides in a cell, tissue or subject, and methods of treatment and/or prevention of various infectious diseases, autoimmune disorders and cancers.
    Type: Application
    Filed: May 6, 2022
    Publication date: August 22, 2024
    Inventors: Phillip Samayoa, Matthew G. Stanton, Raj Rajendran, Debra Klatte, Nathaniel Silver, Luke S. Hamm, Matthew Manganiello, Jeffrey Moffit
  • Publication number: 20240226324
    Abstract: The application describes methods and compositions comprising ceDNA vectors useful for the expression of antibodies and antigen-binding fragments thereof in a cell, tissue or subject, and methods of treatment and/or prevention of various diseases, disorders and cancers.
    Type: Application
    Filed: April 27, 2022
    Publication date: July 11, 2024
    Inventors: Nathaniel Silver, Douglas Anthony Kerr, Phillip Samayoa, Siddharth Jindal, Raphael Gagne
  • Publication number: 20240181085
    Abstract: The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene, e.g., selected from Table 1, encoding a PFIC therapeutic protein (e.g., ATP8B1, ABCB11, ABCB4 or TJP2). Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of PFIC therapeutic protein in vitro, exvivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of PFIC therapeutic protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing PFIC therapeutic protein. Such PFIC therapeutic protein can be expressed for treating a subject with Progressive familial intrahepatic cholestasis (PFIC).
    Type: Application
    Filed: March 18, 2022
    Publication date: June 6, 2024
    Inventors: Ozan Alkan, Douglas Anthony Kerr, Leah Yu Liu, Phillip Samayoa, Nathaniel Silver
  • Publication number: 20240026374
    Abstract: Described herein are ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette comprises a codon optimized nucleic acid sequence encoding a PAH protein, in combination with particular promoter sequences and cis-regulatory elements. Further provided herein are methods and cell lines for reliable gene expression of PAH protein in vitro, ex vivo and in vivo using the ceDNA vectors. Also provided herein are methods and compositions comprising ceDNA vectors useful for the expression of PAH protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing PAH protein. Such PAH protein can be expressed for treating disease, e.g., Phenylketonuria (PKU).
    Type: Application
    Filed: September 16, 2021
    Publication date: January 25, 2024
    Inventors: Leah Yu Liu, Russell Monds, Matthew James Chiocco, Nathaniel Silver, Phillip Samayoa, Douglas Anthony Kerr, Jessica Lynn Keenan
  • Publication number: 20230383311
    Abstract: The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding FVIII protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of FVIII protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are methods and compositions comprising ceDNA vectors useful for the expression of FVIII protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing FVIII protein. Such FVIII protein can be expressed for treating disease, e.g., hemophilia A.
    Type: Application
    Filed: September 16, 2021
    Publication date: November 30, 2023
    Inventors: Debra Klatte, Russell Monds, Luke S. Hamm, Nathaniel Silver, Phillip Samayoa, Douglas Anthony Kerr, Jessica Lynn Keenan
  • Publication number: 20230329244
    Abstract: Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.
    Type: Application
    Filed: January 13, 2023
    Publication date: October 19, 2023
    Inventors: Geoffrey von Maltzahn, Richard Bailey Flavell, Gerardo V. Toledo, Jonathan W. Leff, Phillip Samayoa, Luis Miguel Marquez, David Morris Johnston, Slavica Djonovic, Yves Alain Millet, Craig Sadowski, Jeffrey Lyford, Karen V. Ambrose, Xuecheng Zhang
  • Patent number: 11753618
    Abstract: The present invention relates to methods of scalably producing microorganisms by propagating them within plant tissues and introducing them into agricultural seeds to improve their shelf-life during long-term storage, to produce substances of interest, and to create libraries of microbes.
    Type: Grant
    Filed: December 17, 2020
    Date of Patent: September 12, 2023
    Assignees: Indigo Ag, Inc., AIT Austrian Institute of Technology GMBH
    Inventors: Birgit Mitter, Muhammad Naveed, Teresa Berninger, Stephane Compant, Angela Sessitsch, Geoffrey Von Maltzahn, Richard Bailey Flavell, Gerardo V. Toledo, Slavica Djonovic, Luis Miguel Marquez, David Morris Johnston, Yves Alain Millet, Jeffrey Lyford, Jonathan W. Leff, Phillip Samayoa, Craig Sadowski
  • Publication number: 20230138409
    Abstract: The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding FIX protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of FIX protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of FIX protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing FIX protein. Such FIX protein can be expressed for treating disease, e.g., hemophilia B.
    Type: Application
    Filed: March 24, 2021
    Publication date: May 4, 2023
    Inventors: Douglas Anthony Kerr, Phillip Samayoa, Nathaniel Silver, Sebastian Aguirre, Debra Klatte, Eli Hertzel Pasackow
  • Publication number: 20230134550
    Abstract: The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding GBA protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of GBA protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of GBA protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing GBA protein. Such GBA protein can be expressed for treating disease, e.g., Gaucher disease.
    Type: Application
    Filed: March 24, 2021
    Publication date: May 4, 2023
    Inventors: Douglas Anthony Kerr, Phillip Samayoa, Nathaniel Silver, Luke S. Hamm
  • Patent number: 11570993
    Abstract: Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: February 7, 2023
    Assignee: Indigo Ag, Inc.
    Inventors: Geoffrey von Maltzahn, Richard Bailey Flavell, Gerardo V. Toledo, Jonathan W. Leff, Phillip Samayoa, Luis Miguel Marquez, David Morris Johnston, Slavica Djonovic, Yves Alain Millet, Craig Sadowski, Jeffrey Lyford, Karen V. Ambrose, Xuecheng Zhang
  • Publication number: 20230024354
    Abstract: The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding PAH protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of PAH protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of PAH protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing PAH protein. Such PAH protein can be expressed for treating disease, e.g., Phenylketonuria (PKU).
    Type: Application
    Filed: March 13, 2020
    Publication date: January 26, 2023
    Inventors: Douglas Anthony Kerr, Phillip Samayoa, Nathaniel Silver, Matthew Chiocco
  • Publication number: 20220290186
    Abstract: The application describes ceDNA vectors having linear and continuous structure for gene editing. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a gene editing molecule. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene editing using the ceDNA vectors.
    Type: Application
    Filed: December 6, 2018
    Publication date: September 15, 2022
    Inventors: Robert M. Kotin, Douglas Kerr, Phillip Samayoa, Ozan Alkan, Matthew J. Simmons
  • Patent number: 11357824
    Abstract: Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.
    Type: Grant
    Filed: March 16, 2020
    Date of Patent: June 14, 2022
    Assignee: Axcella Health Inc.
    Inventors: Michael Hamill, Brett Boghigian, Caitlyn Harvey, David Berry, David Young, Geoffrey Von Maltzahn, John Kramarczyk, Jameson Rogers, Kathryn Heard, Michael Doherty, Nathaniel Silver, Phillip Samayoa, Subhayu Basu, Shaila Rahman, Vimal Vaidya, Ying-Ja Chen
  • Publication number: 20220175970
    Abstract: Provided herein are methods and constructs comprising close-ended DNA (ceDNA vectors) for maintaining or sustaining a level of transgene expression at a predetermined level or range for a predefined time, or increasing the level of transgene expression in a cell or a subject, where the transgene expression level can be modulated (e.g., increased) with one or more subsequent administrations (e.g., a re-dose or a booster administration) after an initial priming administration. Provided are methods for personalizing gene therapy throughout an individuals' lifespan to express a transgene at a level that meets an individual's needs, by modulating expression levels of a transgene expressed by ceDNA vector incrementally, or in a step-by-step manner, with one or more administrations after an initial priming administration (e.g., at time 0), thereby enabling titration of the level of expression of the transgene to a desired predetermined expression level or to a desired expression level range.
    Type: Application
    Filed: February 21, 2019
    Publication date: June 9, 2022
    Inventors: Douglas A. Kerr, Matthew G. Stanton, Matt Chiocco, Mark D. Angelino, Robert M. Kotin, Phillip Samayoa
  • Publication number: 20220177545
    Abstract: The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene encoding FVIII protein. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression of FVIII protein in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of FVIII protein in a cell, tissue or subject, and methods of treatment of diseases with said ceDNA vectors expressing FVIII protein. Such FVIII protein can be expressed for treating disease, e.g., hemophilia A.
    Type: Application
    Filed: March 13, 2020
    Publication date: June 9, 2022
    Inventors: Douglas Anthony Kerr, Debra Klatte, Phillip Samayoa, Nathaniel Silver
  • Publication number: 20220119840
    Abstract: Provided herein are methods and constructs related to minimizing immune responses using inhibitors of the immune response, in particular the innate immune response, when administering a desired transgene in a cell achieved by delivery of the transgene with repeated doses of a ceDNA vector.
    Type: Application
    Filed: January 24, 2020
    Publication date: April 21, 2022
    Inventors: Douglas Anthony Kerr, Phillip Samayoa, Robert M. Kotin, Matthew G. Stanton, Ozan Alkan, Matthew Chiocco, Raj Rajendran
  • Patent number: 11254908
    Abstract: This application relates to methods and materials for providing a benefit to a seed, seedling or plant by producing seeds that are internally colonized with endophytes. Beneficial endophytes with particular characteristics are provided. These may be used in the methods described to provide a benefit to a seed, seedling or plant.
    Type: Grant
    Filed: April 8, 2019
    Date of Patent: February 22, 2022
    Assignees: Indigo Ag, Inc., AIT Austrian Institute of Technology GMBH
    Inventors: Birgit Mitter, Muhammad Naveed, Teresa Berninger, Stephane Compant, Angela Sessitsch, Geoffrey Von Maltzahn, Richard Bailey Flavell, Gerardo V. Toledo, Slavica Djonovic, Luis Miguel Marquez, David Morris Johnston, Yves Alain Millet, Jeffrey Lyford, Jonathan W. Leff, Phillip Samayoa, Craig Sadowski
  • Publication number: 20210363480
    Abstract: The present invention relates to methods of scalably producing microorganisms by propagating them within plant tissues and introducing them into agricultural seeds to improve their shelf-life during long-term storage, to produce substances of interest, and to create libraries of microbes.
    Type: Application
    Filed: December 17, 2020
    Publication date: November 25, 2021
    Inventors: Birgit MITTER, Muhammad NAVEED, Teresa BERNINGER, Stephane COMPANT, Angela SESSITSCH, Geoffrey VON MALTZAHN, Richard Bailey FLAVELL, Gerardo V. TOLEDO, Slavica DJONOVIC, Luis Miguel MARQUEZ, David Morris JOHNSTON, Yves Alain MILLET, Jeffrey LYFORD, Jonathan W. LEFF, Phillip SAMAYOA, Craig SADOWSKI
  • Publication number: 20210076685
    Abstract: Materials and methods for improving plant traits and for providing plant fitness benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.
    Type: Application
    Filed: March 23, 2020
    Publication date: March 18, 2021
    Inventors: Karen V. AMBROSE, Nathan A. BILLINGS, Slavica DJONOVIC, Richard Bailey FLAVELL, Trudi A. GULICK, David Morris JOHNSTON, Jonathan W. LEFF, Stephanie M. LIVA, Jeffrey LYFORD, Geoffrey VON MALTZAHN, Luis Miguel MARQUEZ, Yves Alain MILLET, Craig SADOWSKI, Phillip SAMAYOA, Gerardo V. TOLEDO, David R. WEISMAN, Xuecheng ZHANG