Patents by Inventor Phillip Zamore
Phillip Zamore has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9914924Abstract: This invention relates to methods and compositions for treating neurological disease, and more particularly to methods of delivering iRNA agents to neural cells for the treatment of neurological diseases.Type: GrantFiled: January 5, 2015Date of Patent: March 13, 2018Assignee: UNIVERSITY OF MASSACHUSETTSInventors: Neil Aronin, Phillip Zamore
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Publication number: 20150232840Abstract: This invention relates to methods and compositions for treating neurological disease, and more particularly to methods of delivering iRNA agents to neural cells for the treatment of neurological diseases.Type: ApplicationFiled: January 5, 2015Publication date: August 20, 2015Inventors: Neil Aronin, Phillip Zamore
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Publication number: 20070259827Abstract: The present invention provides methods for enhancing discriminatory RNA silencing by RNA silencing agents. In particular, the invention provides methods for generating RNA silencing agents which can discriminate between target and non-target mRNAs that differ in sequence by only one nucleotide. Also provided are improved RNA silencing agents with enhanced discriminatory RNA silencing, e.g., single nucleotide discriminatory RNA silencing. The compositions and methods of the invention are useful in therapeutic strategies for treating genetic disorders associated with dominant, gain-of-function gene mutations.Type: ApplicationFiled: January 25, 2007Publication date: November 8, 2007Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Neil Aronin, Dianne Schwarz, Phillip Zamore
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Publication number: 20070161591Abstract: This invention relates to methods and compositions for treating neurological disease, and more particularly to methods of delivering iRNA agents to neural cells for the treatment of neurological diseases.Type: ApplicationFiled: August 18, 2006Publication date: July 12, 2007Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Neil Aronin, Phillip Zamore
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Publication number: 20070003963Abstract: The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene finction. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response.Type: ApplicationFiled: June 26, 2006Publication date: January 4, 2007Applicants: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, University of Massachusetts Medical Center, Max-Planck-Gesellschaft zur Forderug der Wissenschaften E.V.Inventors: Thomas Tuschl, Phillip Zamore, Phillip Sharp, David Bartel
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Publication number: 20070003960Abstract: The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response.Type: ApplicationFiled: June 26, 2006Publication date: January 4, 2007Applicants: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, University of Massachusetts Medical Center, Max-Planck-Gesellschaft zur Forderug der Wissenschaften E.V.Inventors: Thomas Tuschl, Phillip Zamore, Phillip Sharp, David Bartel
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Publication number: 20070003962Abstract: The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response.Type: ApplicationFiled: June 26, 2006Publication date: January 4, 2007Applicants: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, University of Massachusetts Medical Center, Max-Planck-Gesellschaft zur Forderug der Wissenschaften E. V.Inventors: Thomas Tuschl, Phillip Zamore, Phillip Sharp, David Bartel
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Publication number: 20070003961Abstract: The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response.Type: ApplicationFiled: June 26, 2006Publication date: January 4, 2007Applicants: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, University of Massachusetts Medical Center, Max-Planck-Gesellschaft zur Forderug der Wissenschaften E.V.Inventors: Thomas Tuschl, Phillip Zamore, Phillip Sharp, David Bartel
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Publication number: 20060293267Abstract: The present invention is based, in part, on the discovery that endogenous mRNAs, such as viral miRNAs, can be recruited for translational repression of target mRNAs, such as viral target mRNAs. The RNA-silencing agents and the methods described herein, thereby provide a means of treating viral infections, of treating diseases or disorders caused by viral infections, or for preventing viral propagation. The RNA-silencing agents of the present invention have an mRNA targeting moiety, a linking moiety, and a viral miRNA recruiting moiety.Type: ApplicationFiled: April 13, 2006Publication date: December 28, 2006Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Phillip Zamore, Jennifer Broderick
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Publication number: 20060134787Abstract: The present invention provides methods of enhancing the efficacy and specificity of RNAi using single or double blunt-ended siRNA. The invention also provides single and double-blunt ended siRNA compositions, vectors, and transgenes containing the same for mediating silencing of a target gene. Therapeutic methods are also featured.Type: ApplicationFiled: December 22, 2004Publication date: June 22, 2006Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Phillip Zamore, Dianne Schwarz
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Publication number: 20060009402Abstract: The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.Type: ApplicationFiled: July 12, 2002Publication date: January 12, 2006Inventors: Phillip Zamore, Juanita McLachlan, Gyorgy Hutvagner, Alla Grishok, Craig Mello
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Publication number: 20050256072Abstract: The present invention is based, in part, on the discovery that endogenous mRNAs can be recruited for translational repression of target mRNAs. The RNA-silencing agents and the methods described herein, thereby provide a means by which to treat genetic (e.g., genetic neurodegenerative diseases such as Huntington's Disease) or non-genetic diseases by, for example, blocking the synthesis of proteins that contribute to the diseases. Accordingly the RNA-silencing agents of the present invention have an mRNA targeting moiety, a linking moiety, and an mRNA recruiting moiety.Type: ApplicationFiled: February 9, 2005Publication date: November 17, 2005Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Neil Aronin, Phillip Zamore, Jennifer Broderick
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Publication number: 20050227256Abstract: The present invention relates to the discovery of a method for inhibiting RNA silencing in a target sequence-specific manner. RNA silencing requires a set of conserved cellular factors to suppress expression of gene-encoded polypeptide. The invention provides compositions for sequence-specific inactivation of the RISC component of the RNA silencing pathway, and methods of use thereof. The RISC inactivators of the present invention enable a variety of methods for identifying and characterizing miRNAs and siRNAs, RISC-associated factors, and agents capable of modulating RNA silencing. Therapeutic methods and compositions incorporating RISC inactivators and therapeutic agents identified through use of RISC inactivators are also featured.Type: ApplicationFiled: November 26, 2004Publication date: October 13, 2005Inventors: Gyorgy Hutvagner, Phillip Zamore
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Publication number: 20050214818Abstract: The present invention relates to the discovery of both developmental and RNA interference roles for the armitage (armi) gene and its encoded polypeptide, Armitage (Armi). RNA interference requires a set of conserved cellular factors to suppress gene expression. These factors are the components of the RNAi pathway. The methods described herein are useful for modulating the RNAi pathway—both experimentally and therapeutically—by directedly impacting Armi activity.Type: ApplicationFiled: December 20, 2004Publication date: September 29, 2005Applicant: UNIVERSITY OF MASSACHUSETTSInventors: William Theurkauf, Phillip Zamore
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Publication number: 20050186586Abstract: The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.Type: ApplicationFiled: August 4, 2004Publication date: August 25, 2005Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Phillip Zamore, Gyorgy Hutvagner, Dianne Schwarz, Martin Simard
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Publication number: 20050181382Abstract: The present invention provides methods of enhancing the efficacy and specificity of RNAi. The invention also provides compositions for mediating RNAi. In particular, the invention provides siRNAs, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.Type: ApplicationFiled: June 2, 2004Publication date: August 18, 2005Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Phillip Zamore, Gyorgy Hutvagner, Dianne Schwarz, Martin Simard
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Publication number: 20050037988Abstract: Based at least in part on an understanding of the mechanisms by which small RNAs (e.g., naturally-occurring miRNAs) mediate RNA silencing in plants, rules have been established for determining, for example, the degree of complementarity required between an RNAi-mediating agent and its target, i.e., whether mismatches are tolerated, the number of mismatches tolerated, the effect of the position of the mismatches, etc. Such rules are useful, in particular, in the design of improved RNAi-mediating agents which allow for more exact control of the efficacy of RNA silencing.Type: ApplicationFiled: June 2, 2004Publication date: February 17, 2005Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Phillip Zamore, Guiliang Tang