Abstract: One of the two confined volumes, for example the "supplying" one (2), is provided with a tubular perforating element (3) confined within a space closed by a diaphragm or perforable wall (503); the second confined volume (1) also has a perforable diaphragm (301); the surfaces of the two diaphragms (503, 301) directed towards the external environment are permanently bonded together after which the perforator (3) is manipulated from the outside so as to perforate the two diaphragms, placing in communication, via the tubular duct of the perforator, the confined environment of the supplying volume with that of the second volume.

Abstract: An isolating chamber (1) with an annular connector (5) defines an access opening with one dimension greater than that perpendicular thereto and with an internal wall (5A) of constant section to allow the hermetic sliding of successive closure pieces (7,7X) which have a closure membrane (14,14X), and an annular cutting device (9B) for the membrane and for another membrane joined thereto. Each transfer machine (3) has a passageways slot and a closure membrane (22). The two membranes are designed to adhere to each other during the joining up of the two confined volumes (1,3) and are cut by the annular cutting device (9B) to define a discoid (D) with two at least peripherally joined thicknesses. The discoid can be removed into one of the confined volumes to provide the passage between the two the confined volumes; a subsequent uncut closure piece (7X) can be installed in the connector (5) from the interior of the isolating chamber (1), in order to replace the previous closure piece (7) which thus can be removed.

Abstract: One of the two confined volumes, for example the "supplying" one (2), is provided with a tubular perforating element (3) confined within a space closed by a diaphragm or perforable wall (503); the second confined volume (1) also has a perforable diaphragm (301); the surfaces of the two diaphragms (503, 301) directed towards the external environment are permanently bonded together after which the perforator (3) is manipulated from the outside so as to perforate the two diaphragms, placing in communication, via the tubular duct of the perforator, the confined environment of the supplying volume with that of the second volume.

Abstract: One of the two confined volumes, for example the "supplying" one (2), is provided with a tubular perforating element (3) confined within a space closed by a diaphragm or perforable wall (503); the second confined volume (1) also has a perforable diaphragm (301); the surfaces of the two diaphragms (503, 301) directed towards the external environment are permanently bonded together after which the perforator (3) is manipulated from the outside so as to perforate the two diaphragms, placing in communication, via the tubular duct of the perforator, the confined environment of the supplying volume with that of the second volume.