Patents by Inventor Pierre F. Monsan
Pierre F. Monsan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10017748Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: GrantFiled: June 23, 2016Date of Patent: July 10, 2018Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT NATIONAL DE RECHERCHE AGRONOMIQUE, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSEInventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Publication number: 20160298091Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: ApplicationFiled: June 23, 2016Publication date: October 13, 2016Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT NATIONAL DE RECHERCHE AGRONOMIQUE, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSEInventors: Pierre F. MONSAN, Magali REMAUD-SIMEON, Gabrielle POTOCKI-VERONESE, Claire MOULIS
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Patent number: 9399765Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: GrantFiled: July 14, 2015Date of Patent: July 26, 2016Assignees: Centre National de la Recherche Scientifique, Institut National de Recherche Agronomique, Institut National des Sciences Appliquees de ToulouseInventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Publication number: 20150344853Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: ApplicationFiled: July 14, 2015Publication date: December 3, 2015Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT NATIONAL DE RECHERCHE AGRONOMIQUE, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSEInventors: Pierre F. MONSAN, Magali REMAUD-SIMEON, Gabrielle POTOCKI-VERONESE, Claire MOULIS
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Patent number: 9109207Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: GrantFiled: August 2, 2012Date of Patent: August 18, 2015Assignees: Centre National de la Recherche Scientifique, Institut National de Recherche Agronomique, Institut National des Sciences Appliquees de ToulouseInventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Publication number: 20120289689Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: ApplicationFiled: August 2, 2012Publication date: November 15, 2012Inventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Patent number: 8263380Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: GrantFiled: January 14, 2011Date of Patent: September 11, 2012Assignees: Centre National de la Recherche Scientifique, Institut National de Recherche Agronomique, Institut National des Sciences Appliquees de ToulouseInventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Publication number: 20110178289Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: ApplicationFiled: January 14, 2011Publication date: July 21, 2011Inventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Patent number: 7897373Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: GrantFiled: February 8, 2007Date of Patent: March 1, 2011Assignees: Centre National de la Recherche Scientifique, Institut National de Recherche Agronomique, Institut National des Sciences Appliquees de ToulouseInventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Publication number: 20090258389Abstract: The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the ?-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthsis of ?-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: ApplicationFiled: February 8, 2007Publication date: October 15, 2009Inventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Patent number: 5773256Abstract: Disclosed is a process of enzymatically preparing .alpha.-glucoside esters. First, .alpha.-glucosides are produced by placing an acyclic alcohol or a mixture of acyclic alcohols having a water solubility of at least 2.7% v/v at 20.degree. C. in contact with starch, maltodextrins or maltose, in the presence of a purified enzymatic preparation having .alpha.-transglucosylation activity, wherein the enzymatic preparation is free of .beta.-glucosidase activity. Then, the .alpha.-glucosides are contacted with at least one fatty acid and a preparation having lipase activity to produce the .alpha.-glucoside esters, which may then be recovered. The preparation having .alpha.-transglucosylation activity may originate from a fungus such as Talaromyces duponti, Aspergillus niger, Aspergillus oryzae or Aspergillus batatae.Type: GrantFiled: June 5, 1995Date of Patent: June 30, 1998Assignee: Ulice SAInventors: Vincent P. Pelenc, Francois M.B. Paul, Pierre F. Monsan
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Patent number: 5554508Abstract: A process for the simultaneous enzymatic synthesis of a mixture of alkyl esters of peptides and peptides is presented. In the process, a protein substrate and at least one aliphatic alkanol having from 1 to 5 carbon atoms at a concentration of more than about 30% V/V, preferably 70% V/V to 90% V/V, are treated with a serine protease. A mixture of alkyl esters of peptides and peptides having an esterification value of at least 25% and an average molecular mass of about 3,000 to about 10,000 daltons is recovered. In a further step, the mixture of alkyl esters of peptides and peptides may be precipitated from an alcoholic medium, in an aqueous phase to prepare microparticles. The mixture of alkyl esters of peptides and peptides, or the microparticles may be used in cosmetic, food, pharmaceutical, wetting agent, film-agent, or emollient compositions.Type: GrantFiled: October 31, 1994Date of Patent: September 10, 1996Assignee: UliceInventors: Daniel H. Auriol, Francois B. Paul, Pierre F. Monsan
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Patent number: 5518733Abstract: Cosmetic compositions providing a suitable medium for the development of beneficial endogenous flora, and including at least one oligosaccharide selected from the group consisting of gluco-oligosaccharides, fructo-oligosaccharides, .alpha.- and .beta.-galacto-oligosaccharides and mixtures thereof. Examples of compositions are liquid soap, body lotion, face cream and vaginal gel.Type: GrantFiled: December 23, 1993Date of Patent: May 21, 1996Assignee: BioeuropeInventors: Jean-Pierre H. G. Lamothe, Yves G. Marchenay, Pierre F. Monsan, Francois M. B. Paul, Vincent Pelenc
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Patent number: 5366962Abstract: The present invention concerns the use of fructosyl oligosaccharides as low-calorie sweeteners, and the foods, dietetic products and drinks containing them.Type: GrantFiled: October 9, 1990Date of Patent: November 22, 1994Assignee: Roussel UclafInventors: Jacques Biton, Gerard Gellf, Jean-Marc Michel, Francois B. Paul, Pierre F. Monsan
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Patent number: 5141858Abstract: A method for the production of oligodextrans containing an .alpha.(1.fwdarw.2) bond which uses glycosyltransferase from Leuconostoc mesenteroides, particularly strain B-1299, sucrose and an accepter moiety such as glucose, maltose, isomaltose, isomaltotriose or methyl .alpha.-glucose. Optionally, this product may be further treated with an endodextranase or glucosamylase.Type: GrantFiled: July 27, 1990Date of Patent: August 25, 1992Assignee: BioEuropeInventors: Francois B. Paul, Agustin Lopez Mungica Canales, Magali M. Remaud, Vincent P. Pelenc, Pierre F. Monsan
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Patent number: 4978751Abstract: The present invention concerns the use of fructosyl oligosaccharides as low-calorie sweeteners, and the foods, dietetic products and drinks containing them.Type: GrantFiled: April 13, 1989Date of Patent: December 18, 1990Assignee: Roussel-UlcafInventors: Jacques Biton, Gerard Gellf, Jean-Marc Michel, Francois B. Paul, Pierre F. Monsan
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Patent number: 4956489Abstract: The invention relates to the preparation of water-soluble L-tyrosine derivatives having the formula ##STR1## where R.sub.1 is a L-malyl, L-lactyl, L-glutamyl or L-aspartyl group, R.sub.2 is a --OH, --OM, methoxy, ethoxy or amino group and M is an alkaline metal, by enzymatic condensation of an acid selected among L-malic, L-lactic, L-glutamic and L-aspartic acids, with a L-tyrosine derivatives selected among the ethyl and methyl esters and the amide of L-tyrosine. The enzyme is obtained from a culture of Micrococcus casealyticus. The compounds where R.sub.1 is a L-malyl or L-lactyl group are new. Said L-tyrosine derivatives are useful in cosmetic and pharmaceutical compositions, such as tanning and sun compositions.Type: GrantFiled: October 14, 1988Date of Patent: September 11, 1990Assignee: BioeuropeInventors: Daniel H. Auriol, Francois B. Paul, Pierre F. Monsan
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Patent number: 4950604Abstract: The invention relates to biotechnology.It relates, in particular, to cultures of Klebsiella sp. microorganisms possessing the characteristics of the strain BEC 441 deposited under No. I-714 at the Collection Nationale de Cultures de Micro-organismes (National Collection of Microorganism Cultures) of the Institut Pasteur, as well as strains obtained from the strain BEC 441 by mutation, these strains being capable of producing polysaccharides rich in rhamnose by fermentation in a nutrient medium containing assimilable sources of carbon and of nitrogen and inorganic substances.Application for the production of a mixture of monosaccharides having a high content of rhamnose.Type: GrantFiled: December 8, 1988Date of Patent: August 21, 1990Assignee: BioeuropeInventors: Marianne G. P. Graber-Gubert, Joseph C. A. Morin, Francis L. Duchiron, Pierre F. Monsan
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Patent number: 4861381Abstract: The invention relates to the field of biotechnology.It relates to a process for preparing a syrup, the dry extract of which includes a high content of isomaltose, which comprises treating an aqueous solution of sucrose with a mixture of the enzymes dextransucrase and dextranase at a temperature of 0.degree. to 50.degree. C. and at a pH in the range from 4.5 to 7, so as to obtain an aqueous syrup containing fructose, isomaltose, glucose and, possibly, isomaltotriose as the main constituents.The syrup obtained is a useful intermediate for the synthesis, in particular, of isomaltitol.Type: GrantFiled: July 1, 1987Date of Patent: August 29, 1989Assignee: Sucre Recherches et DeveloppementsInventors: Francois B. Paul, Pierre F. Monsan, Magali M. C. Remaud, Vincent P. Pelenc