Patents by Inventor Pierre Girot

Pierre Girot has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20110313147
    Abstract: Ion exchange and hydrophobic interaction chromatographic materials are constructed by tethering a terminal binding functionality to a solid support via a hydrophobic linker. The backbone of the linker typically comprises sulfur-containing moieties. Suitable terminal binding functionalities are tertiary amines, quaternary ammonium salts, or hydrophobic groups. These chromatographic materials possess both hydrophobic and ionic character under the conditions prescribed for their use. The separation of proteins from crude mixtures at physiological ionic strength can be accomplished with a chromatographic material of this type by applying pH or ionic strength gradients, thereby effecting protein adsorption and desorption.
    Type: Application
    Filed: August 16, 2011
    Publication date: December 22, 2011
    Applicant: PALL CORPORATION
    Inventors: Egisto Boschetti, Pierre Girot
  • Patent number: 8021889
    Abstract: Ion exchange and hydrophobic interaction chromatographic materials are constructed by tethering a terminal binding functionality to a solid support via a hydrophobic linker. The backbone of the linker typically comprises sulfur-containing moieties. Suitable terminal binding functionalities are tertiary amines, quaternary ammonium salts, or hydrophobic groups. These chromatographic materials possess both hydrophobic and ionic character under the conditions prescribed for their use. The separation of proteins from crude mixtures at physiological ionic strength can be accomplished with a chromatographic material of this type by applying pH or ionic strength gradients, thereby effecting protein adsorption and desorption.
    Type: Grant
    Filed: January 14, 2005
    Date of Patent: September 20, 2011
    Assignee: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Patent number: 7815783
    Abstract: Each embodiment includes a central sample reservoir and a plurality of satellite reservoirs. In a first embodiment, a first electrode in electrical contact with the central reservoir is charged and second electrodes in electrical contact with the satellite reservoirs are sequentially charged, thereby pI filtering molecules in the central reservoir into the satellite reservoirs. In a second embodiment, the central reservoir is configured to rotate so that molecules in a sample in the central reservoir are centrifugally pI-filtered into the satellite reservoirs. In a third embodiment, first and second electrodes proximate opposite first and second satellite reservoirs, respectively, are charged. Some molecules in a sample are pI filtered into the first and second satellite reservoirs. Third and fourth electrodes proximate opposite third and fourth satellite reservoirs, respectively, are then charged. Some molecules in a sample are pI filtered into the third and fourth satellite reservoirs.
    Type: Grant
    Filed: March 8, 2005
    Date of Patent: October 19, 2010
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Egisto Boschetti, Lee O. Lomas, Pierre Girot
  • Patent number: 7326776
    Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.
    Type: Grant
    Filed: September 14, 2006
    Date of Patent: February 5, 2008
    Assignee: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Publication number: 20070151910
    Abstract: Ion exchange and hydrophobic interaction chromatographic materials are constructed by tethering a terminal binding functionality to a solid support via a hydrophobic linker. The backbone of the linker typically comprises sulfur-containing moieties. Suitable terminal binding functionalities are tertiary amines, quaternary ammonium salts, or hydrophobic groups. These chromatographic materials possess both hydrophobic and ionic character under the conditions prescribed for their use. The separation of proteins from crude mixtures at physiological ionic strength can be accomplished with a chromatographic material of this type by applying pH or ionic strength gradients, thereby effecting protein adsorption and desorption.
    Type: Application
    Filed: January 14, 2005
    Publication date: July 5, 2007
    Applicant: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Publication number: 20070039891
    Abstract: Proteins can be separated from mixtures, based on their pI values, through the use of a series of chromatographic materials, each comprising a solid buffer and an ion exchange resin. Each solid buffer generates a stable pH, such that passing proteins possess a net charge and can be separated by means of an appropriate ion exchanger. In this fashion, proteins from complex biological fluids can be separated for identification and study.
    Type: Application
    Filed: July 27, 2006
    Publication date: February 22, 2007
    Inventors: Egisto Boschetti, Pier Righetti, Pierre Girot, Frederic Fortis
  • Publication number: 20070015143
    Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.
    Type: Application
    Filed: September 14, 2006
    Publication date: January 18, 2007
    Applicant: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Patent number: 7144743
    Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.
    Type: Grant
    Filed: September 12, 2003
    Date of Patent: December 5, 2006
    Assignee: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Publication number: 20060021941
    Abstract: A new adsorbent of a porous mineral oxide material with apatite crystals, preferably hydroxyapatite crystals, in the pores of the mineral oxide material is disclosed.
    Type: Application
    Filed: September 28, 2005
    Publication date: February 2, 2006
    Applicant: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Publication number: 20050269257
    Abstract: The present invention provides small, dense mineral oxide solid supports or microbeads, comprising dense microporous mineral oxides matrices in which a skin of polymers is rooted, and their use in downstream processing, especially for fluidized bed purification of bioparticles or high molecular weight macromolecules.
    Type: Application
    Filed: July 21, 2005
    Publication date: December 8, 2005
    Applicant: Pall Corporation
    Inventors: Nicolas Voute, Egisto Boschetti, Pierre Girot
  • Patent number: 6972090
    Abstract: A new adsorbent of a porous mineral oxide material with apatite crystals, preferably hydroxyapatite crystals, in the pores of the mineral oxide material is disclosed.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: December 6, 2005
    Assignee: Pall Corporation
    Inventors: Egisto Boschetti, Pierre Girot
  • Publication number: 20050205427
    Abstract: Each embodiment includes a central sample reservoir and a plurality of satellite reservoirs. In a first embodiment, a first electrode in electrical contact with the central reservoir is charged and second electrodes in electrical contact with the satellite reservoirs are sequentially charged, thereby pI filtering molecules in the central reservoir into the satellite reservoirs. In a second embodiment, the central reservoir is configured to rotate so that molecules in a sample in the central reservoir are centrifugally pI-filtered into the satellite reservoirs. In a third embodiment, first and second electrodes proximate opposite first and second satellite reservoirs, respectively, are charged. Some molecules in a sample are pI filtered into the first and second satellite reservoirs. Third and fourth electrodes proximate opposite third and fourth satellite reservoirs, respectively, are then charged. Some molecules in a sample are pI filtered into the third and fourth satellite reservoirs.
    Type: Application
    Filed: March 8, 2005
    Publication date: September 22, 2005
    Applicant: Ciphergen Biosystems, Inc.
    Inventors: Egisto Boschetti, Lee Thomas, Pierre Girot
  • Publication number: 20040124149
    Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.
    Type: Application
    Filed: September 12, 2003
    Publication date: July 1, 2004
    Applicant: Ciphergen Biosystems, Inc.
    Inventors: Egisto Boschetti, Pierre Girot, Pil-Je Um
  • Publication number: 20030218130
    Abstract: The present invention provides a substrate having a polymerized, polysaccharide-based hydrogel attached to the surface. The hydrogel can be derivatized with binding functionalities that bind analytes from a sample. The invention further provides methods of using the device and gels that are capable of selectively binding one or more analytes from a sample.
    Type: Application
    Filed: April 14, 2003
    Publication date: November 27, 2003
    Applicant: Ciphergen Biosystems, Inc.
    Inventors: Egisto Boschetti, Jian Ding, Pierre Girot, Luc Gurrier, Christopher A. Pohl, Michael T. Grimes
  • Patent number: 6613234
    Abstract: The present invention provides porous mineral oxide beads which have large pore volumes and enhanced stability. The beads are based on a tetravalent metal oxide, such as zirconia, titania or hafnia. These highly porous beads are produced from a mixture of tetravalent mineral oxides, mineral pore inducing agents which are oxides or salts of trivalent metals, and optional binders. The porous mineral beads can be filled with a polymer gel and used for adsorption and chromatography applications.
    Type: Grant
    Filed: March 22, 1999
    Date of Patent: September 2, 2003
    Assignee: Ciphergen Biosystems, Inc.
    Inventors: Nicolas Voute, Egisto Boschetti, Pierre Girot, Marc Perez, Jean-Claude Viguie
  • Publication number: 20030125529
    Abstract: A new adsorbent of a porous mineral oxide material with apatite crystals, preferably hydroxyapatite crystals, in the pores of the mineral oxide material is disclosed.
    Type: Application
    Filed: November 27, 2002
    Publication date: July 3, 2003
    Applicant: Ciphergen Biosystems, Inc.
    Inventors: Egisto Boschetti, Pierre Girot
  • Publication number: 20020104801
    Abstract: The present invention provides small, dense mineral oxide solid supports or microbeads, comprising dense microporous mineral oxides matrices in which a skin of polymers is rooted, and their use in downstream processing, especially for fluidized bed purification of bioparticles or high molecular weight macromolecules.
    Type: Application
    Filed: March 22, 1999
    Publication date: August 8, 2002
    Inventors: NICOLAS VOUTE, EGISTO BOSCHETTI, PIERRE GIROT
  • Publication number: 20020005383
    Abstract: The present invention provides porous mineral oxide beads which have large pore volumes and enhanced stability. The beads are based on a tetravalent metal oxide, such as zirconia, titania or hafnia. These highly porous beads are produced from a mixture of tetravalent mineral oxides, mineral pore inducing agents which are oxides or salts of trivalent metals, and optional binders. The porous mineral beads can be filled with a polymer gel and used for adsorption and chromatography applications.
    Type: Application
    Filed: March 22, 1999
    Publication date: January 17, 2002
    Inventors: NICOLAS VOUTE, EGISTO BOSCHETTI, PIERRE GIROT, MARC PEREZ, JEAN-CLAUDE VIGUIE
  • Patent number: 6045697
    Abstract: This invention relates generally to modified porous solid supports and processes for the preparation and use of same. In particular, passivated porous supports are disclosed which are characterized by a reversible high sorptive capacity substantially unaccompanied by non-specific adsorption of or interaction with biomolecules. Passivation is achieved by use of a passivation mixture comprising a main monomer, a passivating agent comprising polyethyleneimine, and a crosslinking agent, which mixture upon polymerization results in the substantial elimination of the undesirable non-specific interaction with biomolecules.
    Type: Grant
    Filed: November 30, 1998
    Date of Patent: April 4, 2000
    Assignee: Life Technologies, Inc.
    Inventors: Pierre Girot, Egisto Boschetti
  • Patent number: 5906734
    Abstract: This invention relates generally to modified porous solid supports and processes for the preparation and use of same. In particular, passivated porous supports are disclosed which are characterized by a reversible high sorptive capacity substantially unaccompanied by non-specific adsorption of or interaction with biomolecules. Passivation is achieved by use of a passivation mixture comprising a main monomer, a passivating agent comprising polyethyleneimine, and a crosslinking agent, which mixture upon polymerization results in the substantial elimination of the undesirable non-specific interaction with biomolecules.
    Type: Grant
    Filed: May 19, 1997
    Date of Patent: May 25, 1999
    Assignee: Biosepra Inc.
    Inventors: Pierre Girot, Egisto Boschetti