Abstract: The invention relates to a device comprising: a laser source emitting a first beam with a central wavelength ?1 lying between 1010 nm and 1050 nm, a spectral supercontinuum generator downstream of the laser source, generating a second beam with a central wavelength ?2 lying between 1670 nm and 1730 nm from a part of the first beam, an optical parametric amplification system downstream of the spectral supercontinuum generator, generating a third beam with a central wavelength ?3 lying between 2540 nm and 2690 nm from at least a part of the second beam and a part of the first beam, and a second harmonic generator downstream of the optical parametric amplification system, the second harmonic generator generating a fourth beam with a central wavelength ?4 lying between 1270 nm and 1345 nm from at least a part of the third beam.

Abstract: The invention relates to a device comprising: a laser source emitting a first beam with a central wavelength ?1 lying between 1010 nm and 1050 nm, a spectral supercontinuum generator downstream of the laser source, generating a second beam with a central wavelength ?2 lying between 1670 nm and 1730 nm from a part of the first beam, an optical parametric amplification system downstream of the spectral supercontinuum generator, generating a third beam with a central wavelength ?3 lying between 2540 nm and 2690 nm from at least a part of the second beam and a part of the first beam, and a second harmonic generator downstream of the optical parametric amplification system, the second harmonic generator generating a fourth beam with a central wavelength ?4 lying between 1270 nm and 1345 nm from at least a part of the third beam.

Abstract: A nonlinear optical microscope is provided, including source of a pulsed laser beam; a spatial light modulator for modulating the spatial profile of the pulsed laser beam; an objective for guiding the modulated beam towards a slide intended to carry a specimen; and a detector for collecting signals originating from the specimen, wherein the spatial light modulator is designed to modulate the intensity and/or the phase of the pulsed laser beam on the rear pupil of the objective to produce a beam that is axially extended and confined in one or two lateral directions after focusing by the objective, and wherein the slide is placed on a motorized stage of a histology slide scanner assembly.

Abstract: A module is provided for a multi-photon imaging system for simultaneously exciting chromophores of a specimen. A first femtosecond laser source emits a first pulsed excitation beam having a repetition rate 1/T and a wavelength ?1 exciting a first chromophores, the absorbed photons originating from the first excitation beam. A second femtosecond laser source emits a second pulsed excitation beam with a wavelength ?2 exciting a second chromophores, the absorbed photons originating from the second beam; the first beam including an “excitation” part exciting the specimen and a “pumping” part acting as a pump beam for exciting the second laser source to synchronize the second laser source with the first laser source. An optical delay line superimposes spatially and temporally the second beam and the excitation part of the first beam to excite at least a third chromophore, the absorbed photons originating from the first and second excitation beams.

Abstract: A nonlinear optical microscope is provided, including source of a pulsed laser beam; a spatial light modulator for modulating the spatial profile of the pulsed laser beam; an objective for guiding the modulated beam towards a slide intended to carry a specimen; and a detector for collecting signals originating from the specimen, wherein the spatial light modulator is designed to modulate the intensity and/or the phase of the pulsed laser beam on the rear pupil of the objective to produce a beam that is axially extended and confined in one or two lateral directions after focusing by the objective, and wherein the slide is placed on a motorized stage of a histology slide scanner assembly.