Patents by Inventor Pierre O. Esteve
Pierre O. Esteve has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11840741Abstract: Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.Type: GrantFiled: November 9, 2021Date of Patent: December 12, 2023Assignee: New England Biolabs, Inc.Inventors: Chaithanya Ponnaluri, Hang-Gyeong Chin, Pierre O. Esteve, Sriharsa Pradhan
-
Publication number: 20230089237Abstract: Compositions, methods and kits are provided for identifying the presence and location of a target in chromosomal DNA. A nicking endonuclease fused to a binding domain that binds to a constant region of an antibody (NEFP) is provided that may be used for binding to a target directly or via an antibody that binds to the target. The target may be a protein or structural feature of the DNA and its presence and location may correspond to a phenotype and/or pathology in a biopsy or other cell sample for diagnostic purposes. The background is reduced by the addition of a glycoaminoglycan (GAG) that reversibly inhibits binding of the NEFP to DNA. Nick translation in the presence of a strand displacing polymerase enables the incorporation of tagged nucleotides that (i) blocks re-nicking; (ii) facilitates immobilization of DNA fragments around the target for sequencing; and/or (iii) enables dye labelling of the chromosomal DNA within the cell nuclei for analysis by microscopy.Type: ApplicationFiled: September 21, 2022Publication date: March 23, 2023Applicant: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Hang Gyeong Chin, George R. Feehery, Shuang-Yong Xu, Vishnu Udayakumaran Nair Sunitha Kumary, Julie Beaulieu, Pierre O. Esteve
-
Patent number: 11492667Abstract: Compositions, methods and kits are provided for identifying the presence and location of a target in chromosomal DNA. A nicking endonuclease fused to a binding domain that binds to a constant region of an antibody (NEFP) is provided that may be used for binding to a target directly or via an antibody that binds to the target. The target may be a protein or structural feature of the DNA and its presence and location may correspond to a phenotype and/or pathology in a biopsy or other cell sample for diagnostic purposes. The background is reduced by the addition of a glycoaminoglycan (GAG) that reversibly inhibits binding of the NEFP to DNA. Nick translation in the presence of a strand displacing polymerase enables the incorporation of tagged nucleotides that (i) blocks re-nicking; (ii) facilitates immobilization of DNA fragments around the target for sequencing; and/or (iii) enables dye labelling of the chromosomal DNA within the cell nuclei for analysis by microscopy.Type: GrantFiled: June 8, 2021Date of Patent: November 8, 2022Assignee: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Hang Gyeong Chin, George R. Feehery, Shuang-Yong Xu, Vishnu Udayakumaran Nair Sunitha Kumary, Julie Beaulieu, Pierre O. Esteve
-
Publication number: 20220056537Abstract: Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.Type: ApplicationFiled: November 9, 2021Publication date: February 24, 2022Applicant: New England Biolabs, Inc.Inventors: Chaithanya Ponnaluri, Hang-Gyeong Chin, Pierre O. Esteve, Sriharsa Pradhan
-
Publication number: 20210388434Abstract: Compositions, methods and kits are provided for identifying the presence and location of a target in chromosomal DNA. A nicking endonuclease fused to a binding domain that binds to a constant region of an antibody (NEFP) is provided that may be used for binding to a target directly or via an antibody that binds to the target. The target may be a protein or structural feature of the DNA and its presence and location may correspond to a phenotype and/or pathology in a biopsy or other cell sample for diagnostic purposes. The background is reduced by the addition of a glycoaminoglycan (GAG) that reversibly inhibits binding of the NEFP to DNA. Nick translation in the presence of a strand displacing polymerase enables the incorporation of tagged nucleotides that (i) blocks re-nicking; (ii) facilitates immobilization of DNA fragments around the target for sequencing; and/or (iii) enables dye labelling of the chromosomal DNA within the cell nuclei for analysis by microscopy.Type: ApplicationFiled: June 8, 2021Publication date: December 16, 2021Applicant: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Hang Gyeong Chin, George R. Feehery, Shuang-Yong Xu, Vishnu Udayakumaran Nair Sunitha Kumary, Julie Beaulieu, Pierre O. Esteve
-
Patent number: 11198910Abstract: Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.Type: GrantFiled: August 31, 2017Date of Patent: December 14, 2021Assignee: New England Biolabs, Inc.Inventors: Chaithanya Ponnaluri, Hang-Gyeong Chin, Pierre O. Esteve, Sriharsa Pradhan
-
Publication number: 20190211404Abstract: Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.Type: ApplicationFiled: August 31, 2017Publication date: July 11, 2019Applicant: New England Biolabs, Inc.Inventors: Chaithanya Ponnaluri, Hang-Gyeong Chin, Pierre O. Esteve, Sriharsa Pradhan
-
Patent number: 9963705Abstract: RNA molecules inhibiting a DNMT and methods and compositions incorporating or generating the RNA molecules are described.Type: GrantFiled: November 10, 2014Date of Patent: May 8, 2018Assignee: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Pierre O. Esteve, Guoqiang Zhang
-
Publication number: 20160272977Abstract: RNA molecules inhibiting a DNMT and methods and compositions incorporating or generating the RNA molecules are described.Type: ApplicationFiled: November 10, 2014Publication date: September 22, 2016Applicant: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Pierre O. Esteve, Guoqiang Zhang