Abstract: A bioreactor system (1A; 1B) includes a variable volume container (3) having a flexible outer wall (27, 29) defining a cell-culture chamber for containing a fluid culture medium, and anchorage dependent cells in suspension. A agitator unit of the system (1A; 1B) is in fluid communication with first, second and third fluid ports (43, 45, 47A; 47B) for performing controlled replenishment of the culture medium in the cell-culture chamber, to admit fresh nutritive culture medium into the cell-culture chamber and for allowing draining of waste culture medium from the cell-culture chamber. The agitator unit further has a roller (15) for pinching the outer wall of the variable volume container (3) to thereby increase its a volume that forms the cell-culture chamber, starting from the initial minimum volume area (23), and means for gently agitating the cell-culture chamber.

Abstract: A method and device to adjust the concentration of at least two gasses in a fluid, wherein the method involves adjusting the concentration of a first gas in the fluid by adjusting the concentration of a second gas in the fluid wherein the first and second gases are kept separate until dissolved in the fluid.

Abstract: The present invention relates to a method and device to adjust the concentration of at least two gasses in a fluid. The method comprises the steps of a) adjusting the concentration of a first gas in the fluid by a means for adjusting a first gas concentration, and b) adjusting the concentration of a second gas in the fluid by a means for adjusting a second gas concentration, wherein the means for adjusting the concentration of the first gas and the means for adjusting the concentration of the second gas are separate from each other.

Abstract: A bioreactor system (1A; 1B) includes a variable volume container (3) having a flexible outer wall (27, 29) defining a cell-culture chamber for containing a fluid culture medium, and anchorage dependent cells in suspension. A agitator unit of the system (1A; 1B) is in fluid communication with first, second and third fluid ports (43, 45, 47A; 47B) for performing controlled replenishment of the culture medium in the cell-culture chamber, to admit fresh nutritive culture medium into the cell-culture chamber and for allowing draining of waste culture medium from the cell-culture chamber. The agitator unit further has a roller (15) for pinching the outer wall of the variable volume container (3) to thereby increase its a volume that forms the cell-culture chamber, starting from the initial minimum volume area (23), and means for gently agitating the cell-culture chamber.