Patents by Inventor Pietro Gatti-Lafranconi

Pietro Gatti-Lafranconi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230356230
    Abstract: Reagent cartridges and related systems and methods for controlling reagent temperature are disclosed. In accordance with an implementation, an apparatus includes a system and a reagent cartridge. The system includes a reagent cartridge receptacle, a non-contact temperature controller, a processor operatively coupled to the temperature controller. The reagent cartridge is receivable within the reagent cartridge receptacle and includes a flow cell assembly, a plurality of reagent reservoirs, and a manifold assembly. The manifold assembly includes a common fluidic line and a plurality of reagent fluidic lines. Each of the plurality of reagent fluidic lines is adapted to be fluidically coupled to a corresponding reagent reservoir and selectively couplable to the common fluidic line. The processor is to cause the temperature controller to change a temperature of at least one of the common fluidic line or one or more of the reagent fluidic lines.
    Type: Application
    Filed: March 2, 2022
    Publication date: November 9, 2023
    Inventors: Elisabet Rosas, Philip Balding, Cyril Delattre, Jacqueline Weir, Ramir Vega, Jennifer Foley, Paul Sangiorgio, Darren Segale, Pietro Gatti Lafranconi, Tarun Khurana, Jacob Ruden, Craig Hetherington
  • Patent number: 11767558
    Abstract: Polynucleotide sequencing methods include incubating unlabeled nucleotides with a cluster of template polynucleotide strands having the same sequence when the identity of the previously added labeled nucleotide is being detected. The detection step provides time for the addition of the unlabeled nucleotides to be incorporated into the copy strands in which the previously added labeled nucleotide did not get incorporated. Thus, at the end of the detection step, all or most of the copy strands will be in phase and ready to incorporate the appropriate labeled nucleotide in the subsequence incorporate step.
    Type: Grant
    Filed: December 5, 2019
    Date of Patent: September 26, 2023
    Assignee: Illumina Cambridge Limited
    Inventors: Pietro Gatti-Lafranconi, Philip Balding
  • Publication number: 20230279385
    Abstract: A variety of different types of targeted transposome complexes are described herein that may be used to mediate sequence-specific targeted transposition of nucleic acids.
    Type: Application
    Filed: February 16, 2023
    Publication date: September 7, 2023
    Applicants: Illumina, Inc., Illumina Cambridge Limited
    Inventors: Frank J. STEEMERS, Jonathan Mark BOUTELL, Pietro GATTI LAFRANCONI, Oliver Jon MILLER, Emma BELL, Sebastien Georg Gabriel RICOULT, Niall Anthony GORMLEY, Kim SCHNEIDER
  • Publication number: 20230242982
    Abstract: A polynucleotide sequencing method comprises (i) removing a label and a blocking moiety from a blocked, labeled nucleotide incorporated into a copy polynucleotide strand that is complementary to at least a portion of a template polynucleotide strand; and (ii) washing the removed label and blocking moiety away from the copy strand with a wash solution comprising a first buffer comprising a scavenger compound. Removing the label and blocking moieties may comprise chemically removing the moieties. The first buffer may also comprise an antioxidant and may be used in a scanning buffer used during a nucleotide detection step.
    Type: Application
    Filed: February 6, 2023
    Publication date: August 3, 2023
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Pietro Gatti-Lafranconi, Helen Sansom, Matthew Hancock
  • Publication number: 20230212667
    Abstract: Polynucleotide sequencing methods for sequencing one or more polynucleotide templates use primers bound to a surface as sequencing primers. The surface primers may include at least a portion of a surface oligonucleotide used during cluster formation. The sequencing methods may be used for single stranded sequencing or double stranded sequencing. Double stranded sequencing methods may employ an enzyme that has nick-translation activity. A kit includes all the reagents needed for sequencing does not include sequencing primers. The kit may be used to accomplish the sequencing methods of the present disclosure.
    Type: Application
    Filed: December 28, 2022
    Publication date: July 6, 2023
    Applicants: ILLUMINA CAMBRIDGE LIMITED, ILLUMINA, INC.
    Inventors: Jonathan Mark Boutell, Eli M. Carrami, Pietro Gatti Lafranconi, Philip Balding, Oliver Jon Miller, Kay Klausing, Justin Robbins, Xiaolin Wu
  • Publication number: 20230137106
    Abstract: The present disclosure is concerned with compositions and methods for the paired-end sequencing of target nucleic acids, and more particularly to obtaining nucleotide sequence information from two separate regions of target nucleic acids using amplification sites having a single type of surface primer.
    Type: Application
    Filed: December 27, 2022
    Publication date: May 4, 2023
    Applicant: Illumina Cambridge Limited
    Inventors: Jonathan Mark Boutell, Pietro Gatti-Lafranconi
  • Patent number: 11634765
    Abstract: The present disclosure is concerned with compositions and methods for the paired-end sequencing of target nucleic acids, and more particularly to obtaining nucleotide sequence information from two separate regions of target nucleic acids using amplification sites having a single type of surface primer.
    Type: Grant
    Filed: December 17, 2019
    Date of Patent: April 25, 2023
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Jonathan Mark Boutell, Pietro Gatti-Lafranconi
  • Patent number: 11584962
    Abstract: A polynucleotide sequencing method comprises (i) removing a label and a blocking moiety from a blocked, labeled nucleotide incorporated into a copy polynucleotide strand that is complementary to at least a portion of a template polynucleotide strand; and (ii) washing the removed label and blocking moiety away from the copy strand with a wash solution comprising a first buffer comprising a scavenger compound. Removing the label and blocking moieties may comprise chemically removing the moieties. The first buffer may also comprise an antioxidant and may be used in a scanning buffer used during a nucleotide detection step.
    Type: Grant
    Filed: December 11, 2019
    Date of Patent: February 21, 2023
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Pietro Gatti-Lafranconi, Helen Sansom, Matthew Hancock
  • Publication number: 20220145379
    Abstract: An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3? OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.
    Type: Application
    Filed: November 25, 2020
    Publication date: May 12, 2022
    Inventors: Wayne N. George, Brian D. Mather, Andrew A. Brown, Pietro Gatti Lafranconi, Maria Candelaria Rogert Bacigalupo, Antoine Francais, Xiaohai Liu
  • Publication number: 20210187470
    Abstract: Provided is a nanoparticle including a scaffold, a single template site for bonding a template polynucleotide to the scaffold, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold, wherein the scaffold is selected from an asymmetrical acrylamide polymer one or a dendrimer including lysyl constitutional repeating units, the single template site for bonding a template polynucleotide to the scaffold is selected from a covalent template bonding site and a noncovalent template bonding site and the plurality of accessory sites for bonding accessory oligonucleotides to the scaffold are selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites. Also provided are methods of using the nanoparticle.
    Type: Application
    Filed: December 22, 2020
    Publication date: June 24, 2021
    Applicants: LLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.
    Inventors: Xiangyuan YANG, Wayne N. GEORGE, Pietro GATTI LAFRANCONI, Yin Nah TEO, Andrew A. BROWN, Maria Rogert BACIGALUPO, Eric BRUSTAD, Hassan BOHRA, Clifford WANG
  • Publication number: 20210187469
    Abstract: Provided is a nanoparticle including a scaffold, a single template site for bonding a template polynucleotide to the scaffold, and a plurality of accessory sites for bonding accessory oligonucleotides to the scaffold, wherein the scaffold is selected from one or more scaffold DNA molecules and one or more scaffold polypeptides, the single template site for bonding a template polynucleotide to the scaffold is selected from a covalent template bonding site and a noncovalent template bonding site and the plurality of accessory sites for bonding accessory oligonucleotides to the scaffold are selected from covalent accessory oligonucleotide bonding sites and noncovalent accessory oligonucleotide bonding sites. Also provided are methods of using the nanoparticle.
    Type: Application
    Filed: December 22, 2020
    Publication date: June 24, 2021
    Applicants: ILLUMINA, INC., ILLUMINA CAMBRIDGE LIMITED, ILLUMINA SINGAPORE PTE. LTD.
    Inventors: Wayne N. GEORGE, Jonathan Mark BOUTELL, Mathieu LESSARD-VIGER, Saurabh NIRANTAR, Pietro GATTI LAFRANCONI, Seth MCDONALD, Yin Nah TEO, Maria Rogert BACIGALUPO, Sergio PEISAJOVICH, Eric BRUSTAD, Michael HOWARD, Xiangyuan YANG, M. Shane Bowen, Alex NEMIROSKI, Jeffrey BRODIN, Ludovic VINCENT, Lorenzo BERTI, Andrew A. BROWN, Fei SHEN, Lena STORMS, Olivia BENICE
  • Publication number: 20200199666
    Abstract: Polynucleotide sequencing methods employ a sequencing oligonucleotide that hybridizes to a free 3? end potion of a template polynucleotide strand with greater affinity than a surface oligonucleotide. Such sequencing oligonucleotides may be used as a primer to determine the sequence of an index sequence by extending the sequencing oligonucleotide using the template strand as a template. Sequencing processes that employ such sequencing oligonucleotides provide a sufficiently intense signal to determine to the sequence of the index sequence.
    Type: Application
    Filed: December 16, 2019
    Publication date: June 25, 2020
    Applicant: Illumina Cambridge Limited
    Inventors: Pietro Gatti-Lafranconi, Philip Balding, Jonathan Mark Boutell
  • Publication number: 20200190569
    Abstract: A polynucleotide sequencing method comprises (i) removing a label and a blocking moiety from a blocked, labeled nucleotide incorporated into a copy polynucleotide strand that is complementary to at least a portion of a template polynucleotide strand; and (ii) washing the removed label and blocking moiety away from the copy strand with a wash solution comprising a first buffer comprising a scavenger compound. Removing the label and blocking moieties may comprise chemically removing the moieties. The first buffer may also comprise an antioxidant and may be used in a scanning buffer used during a nucleotide detection step.
    Type: Application
    Filed: December 11, 2019
    Publication date: June 18, 2020
    Applicant: Illumina Cambridge Limited
    Inventors: Pietro Gatti-Lafranconi, Helen Sansom, Matthew Hancock
  • Publication number: 20200190578
    Abstract: The present disclosure is concerned with compositions and methods for the paired-end sequencing of target nucleic acids, and more particularly to obtaining nucleotide sequence information from two separate regions of target nucleic acids using amplification sites having a single type of surface primer.
    Type: Application
    Filed: December 17, 2019
    Publication date: June 18, 2020
    Applicant: Illumina Cambridge Limited
    Inventors: Jonathan Mark Boutell, Pietro Gatti-Lafranconi
  • Publication number: 20200190576
    Abstract: Polynucleotide sequencing methods include incubating unlabeled nucleotides with a cluster of template polynucleotide strands having the same sequence when the identity of the previously added labeled nucleotide is being detected. The detection step provides time for the addition of the unlabeled nucleotides to be incorporated into the copy strands in which the previously added labeled nucleotide did not get incorporated. Thus, at the end of the detection step, all or most of the copy strands will be in phase and ready to incorporate the appropriate labeled nucleotide in the subsequence incorporate step.
    Type: Application
    Filed: December 5, 2019
    Publication date: June 18, 2020
    Applicant: Illumina Cambridge Limited
    Inventors: Pietro Gatti-Lafranconi, Philip Balding