Patents by Inventor Pingfang Liu

Pingfang Liu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Proteinases with improved properties
    Patent number: 11390862
    Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.
    Type: Grant
    Filed: December 21, 2020
    Date of Patent: July 19, 2022
    Assignee: New England Biolabs, Inc.
    Inventors: Minyong Chen, James C. Samuelson, Ming-Qun Xu, Aihua Zhang, Margaret Heider, Pingfang Liu
  • Proteinases with Improved Properties
    Publication number: 20210115427
    Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.
    Type: Application
    Filed: December 21, 2020
    Publication date: April 22, 2021
    Applicant: New England Biolabs, Inc.
    Inventors: Minyong Chen, James C. Samuelson, Ming-Qun Xu, Aihua Zhang, Margaret Heider, Pingfang Liu
  • Proteinases with Improved Properties
    Publication number: 20200199565
    Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.
    Type: Application
    Filed: December 18, 2019
    Publication date: June 25, 2020
    Applicant: New England Biolabs, Inc.
    Inventors: Minyong Chen, James C. Samuelson, Ming-Qun Xu, Aihua Zhang, Margaret Heider, Pingfang Liu
  • Proteinases with improved properties
    Patent number: 10633644
    Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: April 28, 2020
    Assignee: New England Biolabs, Inc.
    Inventors: Minyong Chen, James C. Samuelson, Ming-Qun Xu, Aihua Zhang, Margaret Heider, Pingfang Liu
  • Method for Reducing Sequencing Errors Caused by DNA Fragmentation
    Publication number: 20180051341
    Abstract: Provided herein, among other things, is a method for reducing sequencing errors caused by mechanical DNA fragmentation. In some embodiments, this method may comprise: (a) obtaining a DNA sample; (b) mechanically fragmenting the DNA sample to produce a template; (c) treating the fragmented DNA with a plurality of DNA repair enzymes; and (d) obtaining a sequence of the fragmented DNA. The treatment step (c) reduces the number of fragmentation induced errors.
    Type: Application
    Filed: January 24, 2017
    Publication date: February 22, 2018
    Applicant: New England Biolabs, Inc.
    Inventors: Thomas C. Evans, Jr., Laurence Ettwiller, Lixin Chen, Pingfang Liu