Abstract: The present disclosure provides a method for preparing a composition including mesenchymal stem cells, extracellular vesicles produced by the mesenchymal stem cells, and growth factors, the composition prepared by the method, and use of the composition for treating arthritis. The composition of the present disclosure achieves the effect of treating arthritis through various efficacy experiments.

Abstract: The present disclosure provides a method for treating liver cirrhosis by using a composition including mesenchymal stem cells, extracellular vesicles produced by the mesenchymal stem cells, and growth factors. The composition of the present disclosure achieves the effect of treating liver cirrhosis through various efficacy experiments.

Abstract: A detection method of antigen-antibody reaction, including: Providing an antibody solution, including an antibody and a type of metal nanoparticle, the type of metal nanoparticle forms bond with the antibody. Adding an antigen to the antibody solution to form a mixed solution. Providing a light beam to the mixed solution, wherein part of the light beam is scattered by the type of metal nanoparticle to form a scattered light beam. Detecting the scattered light beam to determine whether an antigen-antibody reaction occurs.

Abstract: The present invention discloses a method for simultaneously detecting multiple small nucleic acids, which comprises steps: mixing a specimen, fluorescent probes, and bridge nucleic acids having different lengths to form a tested liquid; hybridizing the mixed short nucleic acid molecules, probes and bridge nucleic acids; adding ligases to enable the ligations of the short nucleic acid molecules and the fluorescent probes with the bridge nucleic acids being the templates; injecting the tested liquid into a capillary, and applying a voltage to the capillary to generate an electrophoresis effect and separate the hybridization products; and using laser to induce different fluorescent rays from different reaction products, and measuring the fluorescent rays, whereby the present invention can simultaneously detect multiple types of short nucleic acid molecules in a single capillary.

Abstract: The present invention discloses a quantitative analysis method for microRNAs, wherein a fluorescence-labeled DNA probe, which is equinumerous and completely complementary to a microRNA, hybridize with the microRNA. The products of hybridization include the fluorescence-labeled DNA probe containing 22 nucleotides and the probe-microRNA duplex containing 22 base pairs. The products of hybridization is introduced into a capillary by the pressure difference between two ends of the capillary and the siphon effect and separated by electrophoresis. A laser is used to induce fluorescence from the products of hybridization. Then, the intensities of fluorescence are measured and analyzed.