Abstract: A permeable electrochemical reactive biobarrier used to prohibit the diffusion of groundwater contaminated by organic compounds is disclosed. The permeable electrochemical reactive biobarrier includes at least a conductive fiber layer and at least a cathode. The conductive fiber layer is applied with a proper voltage, and the cathode is disposed at one side of the conductive fiber layer. Herein, the conductive fiber layer can be used as the electron acceptor for respiration occurred by local soil microorganisms, which grow on the surface of the conductive fiber layer. Accordingly, the biodegradation of organic compounds can be continued without additional oxidants.