Abstract: Cap arrangements for per-well fluidics and gas exchange for microplate, microtiter, and microarray technologies are presented for use with cell cultures or other applications. In example implementations, each individual well within in a conventional or specialized microplate can be fully or partially isolated with capping or other arrangements which can include conduits for controlled introduction, removal, and/or exchange of fluids and/or gases. Conduit networks can include small controllable valves that operate under software control, and micro-scale pumps can also be included. Conduit interconnections can include one or more of controllable-valve distribution buses, next-neighbor interconnections, and other active or passive interconnection topologies.

Abstract: A color optical tomography system with fluorescence capabilities includes a light emitting array having a plurality of light emitting elements, a sample module, and a light sensing array including a plurality of light sensing elements, wherein the light sensing array is configured to sense light emitted from the light emitting array which has passed through the sample module. Light emitting elements emitting in the visible and UV ranges can comprise light emitting diodes (LEDs), organic light emitting diodes (OLEDs), organic light emitting transistors (OLETs), and/or other optoelectronic devices. The light sensing array can comprise for example organic light sensing devices, photodiodes, phototransistors, a CMOS photodetector, and/or charge-coupled devices (CCI)s). The light emitting array and the light sensing array can he flat or curved.

Abstract: This invention provides novel statistical processing approaches on measurements from diverse or varied sensors to collectively implement high-accuracy testing for detections and/or discernments of pathogens, substances, and/or biomarkers. For example, a group of inexpensive sensors can approach, match, or exceed the performance of existing high-accuracy methods. In an implementation, measurements are obtained from disposable optical and/or bioFET sensors arrayed on a substrate using printing techniques. In an example application, the invention operates together with or within a platform technology comprising a rich ability to flexibly perform, create, deploy, maintain, and update a range of panels, assay, array, and/or test sequences for a wide range of substances and pathogens using a wide range and large number of collocated highly-selective sensors and supplemental sensors (such as temperature, pH, selective ions, etc.).

Abstract: A “breadboard” approach by which a biochemical pathway under study is separated or segmented into smaller portions, at least one of which can to a degree of approximation be accurately emulated with a replica microscale and/or nanoscale fluidic implementation whose constituent species, inhibitor(s), catalyst(s) and other reaction agent(s) can be closely controlled and at least one aspect of whose behavior can be measured. Control and measurement information interfaces with a computer that executes algorithms comprising one or more of a control process, control event-script, experiment, data recording, and mathematical model. A model can be used to simulate the actions, behavior, or other aspects of another portion of the biochemical signaling process, pathway, or network.

Abstract: Per-well imaging arrangements for microplate, microtiter, and microarray technologies are presented. In example implementations, each individual well within in a conventional or specialized microplate can be fully or partially isolated with capping or other arrangements which can include conduits for controlled introduction, removal, and/or exchange of fluids and/or gases. Conduit networks can include small controllable valves that operate under software control, and micro-scale pumps can also be included. Conduit interconnections can include one or more of controllable-valve distribution buses, next-neighbor interconnections, and other active or passive interconnection topologies. Cap arrangements can include or provide one or more sensors of various types, including but not limited to selective gas sensors, chemical sensors, temperature sensors, pH sensors, biosensors, immunosensors, molecular-imprint sensors, optical sensors, fluorescence sensors, bioFETS, etc.

Abstract: Expanded cell culture incubator arrangements for interfacing and supporting advanced microplate, microarray, and microtiter technologies comprising per-well fluidics, gas exchange, electronic sensors, and imaging features are presented. In example implementations, each individual well of a conventional or specialized microplate can be fully or partially isolated with capping or other arrangements. Specialized microplate and/or capping arrangements can include conduits for controlled introduction, removal, and/or exchange of fluids and/or gases. Conduit networks can include small controllable valves that operate under software control, and micro-scale pumps can also be included. Conduit interconnections can include one or more of controllable-valve distribution buses, next-neighbor interconnections, and other active or passive interconnection topologies.

Abstract: Arrangements for per-well illumination and optical stimulation arrangements for microplate, microtiter, and microarray technologies are presented. In example implementations, each individual well within in a conventional or specialized microplate can be fully or partially isolated with capping or other arrangements which can include conduits for controlled introduction, removal, and/or exchange of fluids and/or gases. Conduit networks can include small controllable valves that operate under software control, and micro-scale pumps can also be included. Conduit interconnections can include one or more of controllable-valve distribution buses, next-neighbor interconnections, and other active or passive interconnection topologies. Cap arrangements can include or provide one or more sensors of various types, including but not limited to selective gas sensors, chemical sensors, temperature sensors, pH sensors, biosensors, immunosensors, molecular-imprint sensors, optical sensors, fluorescence sensors, bioFETS, etc.

Abstract: Arrangements for per-well electronic sensors for microplate, microtiter, and microarray technologies are presented. In example implementations, each individual well within in a conventional or specialized microplate can be fully or partially isolated with capping or other arrangement. Cap arrangements can include or provide one or more sensors of various types, including but not limited to selective gas sensors, chemical sensors, temperature sensors, pH sensors, biosensors, immunosensors, molecular-imprint sensors, optical sensors, fluorescence sensors, bioFET sensor, etc. Incubator interfacing and imaging are also described. The caps can also include conduits for controlled introduction, removal, and/or exchange of fluids and/or gases. Conduit networks can include small controllable valves that operate under software control, and micro-scale pumps can also be included.

Abstract: Cap arrangements for per-well fluidics and gas exchange for microplate, microtiter, and microarray technologies are presented for use with cell cultures or other applications. In example implementations, each individual well within in a conventional or specialized microplate can be fully or partially isolated with capping or other arrangements which can include conduits for controlled introduction, removal, and/or exchange of fluids and/or gases. Conduit networks can include small controllable valves that operate under software control, and micro-scale pumps can also be included. Conduit interconnections can include one or more of controllable-valve distribution buses, next-neighbor interconnections, and other active or passive interconnection topologies.

Abstract: Arrangements for per-well fluidics, gas exchange, and electronic sensors for microplate, microtiter, and microarray technologies are presented. In example implementations, each individual well within in a conventional or specialized microplate can be fully or partially isolated with capping or other arrangements which can include conduits for controlled introduction, removal, and/or exchange of fluids and/or gases. Conduit networks can include small controllable valves that operate under software control, and micro-scale pumps can also be included. Conduit interconnections can include one or more of controllable-valve distribution buses, next-neighbor interconnections, and other active or passive interconnection topologies. Cap arrangements can include or provide one or more sensors of various types, including but not limited to selective gas sensors, chemical sensors, temperature sensors, pH sensors, biosensors, immunosensors, molecular-imprint sensors, optical sensors, fluorescence sensors, bioFETS, etc.

Abstract: An optical tomography system includes a light emitting array having a plurality of light emitting elements, a sample module, and a light sensing array including a plurality of light sensing elements, wherein the light sensing array is configured to sense light emitted from the light emitting array which has passed through the sample module. The light emitting elements can comprise for example light emitting diodes (LEDs), organic light emitting diodes (OLEDs), organic light emitting transistors (OLETs), and/or other optoelectronic devices. The light sensing array can comprise for example organic light sensing devices, photodiodes, phototransistors, a CMOS photodetector, and/or charge-coupled devices (CCDs). The light emitting array can be flat or curved, and the light sensing array can be flat or curved. The collection of measurement values can be overspecified, and a generalized inverse operation can be used to provide a solution to a resulting overspecified system of equations.

Abstract: An electronic imaging flow-microscope for remote environmental sensing, bioreactor process monitoring, and optical microscopic tomography applications is described. A fluid conduit has a port on each end of a thin flat transparent fluid transport region. A planar illumination surface contacts one flat side of the transparent fluid transport region and a planar image sensing surface contacts the other flat side. Light from the illumination surface travels through the transparent fluid transport region to the planar image sensing surface, producing a light field affected by the fluid and objects present. The planar image sensing surface creates electrical image signals responsive to the light field. The planar illumination surface can be light emitting elements such as LEDs, OLEDs, or OLET whose illumination can be sequenced in an image formation process. The flow microscope can further comprise flow-restricting valves, pumps, energy harvesting arrangements, and power management.

Abstract: A cell incubation system and methods for using a cell incubation system are described. The incubator system comprises an incubation compartment for receiving a microplate for processing samples. The microplate comprises a plurality of wells, each of the plurality of wells including a collection chamber, and a plurality of fluidic structures coupled to the plurality of wells. The microplate further comprises a plurality of sensors coupled to the plurality of wells.

Abstract: A mobile microscopy apparatus usable in connection with a mobile computing device comprising a memory unit and camera, the mobile microscopy apparatus comprising: an illumination module for illuminating a removable media with an illuminating light, an image acquisition optics for creating an image of the sample for acquisition by the camera of the mobile computing device, and a mounting frame assembly for detachably mounting the illumination module and the image acquisition optics to the mobile computing device and for holding the removable media in a predetermined position. In various implementations, the illumination module may provide either backside or frontside illumination of the removable media using the light generated by a light source of the mobile computing device. The mobile microscopy apparatus may operate in the microscope configuration, visible colormatic microarray configuration, and/or fluorescent microarray configuration.

Abstract: The invention provides a platform technology with rich ability to flexibly perform, create, deploy, maintain, and update a wide range of panels, assay, array, and/or sequence of tests for a wide range of substances and pathogens. The invention provides a unifying framework for widely-ranging miniature sensor implementation, fluidic/gas interfacing, electrical interfaces and optical interfaces, and further by collocating, allowing the integration a large number highly-selective sensors and chemical sensors—together as needed with appropriately selected supplemental sensors (for example temperature, pH, selective ions, etc.), into a common readily-manufacturable framework. The diverse sensor arrays give rise to statistical enhancing through novel statistical processing approaches.

Abstract: Nitric Oxide (NO) acts as double-edged sword, which induces and prevents cell death, depending on various factors. The mechanism for the NO regulation of cells is not fully understood. The present invention provides experiment design methods and therapy design methods leveraging viable hypothesis supported by current research findings. This invention determines the effects of NO by controlling the majority parameters such as the steady-state concentration of NO, duration of NO exposure and the local level of oxygen. The experiment designs are structured to improve understanding of NO regulation of cells, and further can be directly adapted for therapy design. The invention directs these experiment design and therapy design methods to the study and treatment of cancer. In an application, the effects of exogenous NO on inducible Nitric Oxide Synthase (iNOS) expression and signal transduction in ovarian cancer is applied to drug discovery and therapy design for ovarian and other cancers.