Patents by Inventor Poul Erik Pedersen
Poul Erik Pedersen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240101988Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.Type: ApplicationFiled: July 19, 2023Publication date: March 28, 2024Applicant: Novozymes A/SInventors: Carsten Andersen, Poul Erik Pedersen, Michael Dolberg Rasmussen, Carsten Hjort
-
Publication number: 20230265408Abstract: The present invention relates to protease variants, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising said polynucleotides, host cells expressing said variants, methods of obtaining the variants, detergent compositions comprising said variants, and use of said variants or said detergent compositions.Type: ApplicationFiled: August 30, 2021Publication date: August 24, 2023Applicant: Novozymes A/SInventors: Rolf Thomas Lenhard, Esben Peter Friis, Poul Erik Pedersen, Rasmus Lund Andersen
-
Publication number: 20210380667Abstract: Disclosed are protease inhibitors capable of inhibiting the protease activity of a S1 or a S8 protease at ambient pH, but where the inhibitor further has a pH dependent binding to the S1 or the S8 inhibitor meaning that a complex of a S1 and a S8 protease and the inhibitor dissociates when pH is lowered to a pH below 6.0, e.g. to a pH value in the range of 4.0 to 6.0; e.g. in the range of 4.5 to 6.0; e.g. in the range of 4.5 to 5.5; whereby the protease activity is released.Type: ApplicationFiled: October 8, 2019Publication date: December 9, 2021Applicant: Novozymes A/SInventors: Poul Erik Pedersen, Esben Peter Friis, Carsten Andersen, Peter Rahbek Oestergaard, Johan Qvist
-
Publication number: 20210017544Abstract: The present invention relates to a method for counter-selection by inhibition conditionally essential genes.Type: ApplicationFiled: December 12, 2008Publication date: January 21, 2021Applicant: Novozymes A/SInventors: Michael Dolberg Rasmussen, Poul Erik Pedersen
-
Publication number: 20200318086Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.Type: ApplicationFiled: November 7, 2018Publication date: October 8, 2020Applicant: Novozymes A/SInventors: Carsten Anderson, Poul Erik Pedersen, Michael Dolberg Rasmussen, Carsten Hjort
-
Patent number: 10640757Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.Type: GrantFiled: September 23, 2013Date of Patent: May 5, 2020Assignee: Novozymes A/SInventors: Steen Troels Jorgensen, Poul Erik Pedersen
-
Publication number: 20190185847Abstract: The present invention relates to methods for improving at least one property of a microorganism host cell by repressing the expression of one or more genome target sequence of interest by CRISPR-inhibition as well as the resulting host cells and methods of production emplying said host cells.Type: ApplicationFiled: July 5, 2017Publication date: June 20, 2019Applicant: Novozymes A/SInventors: Michael Dolberg Rasmussen, Poul Erik Pedersen, Carsten Hjort, Barbara Cherry
-
Publication number: 20170175098Abstract: The present invention relates to enzyme variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of using the variants and composition comprising variants.Type: ApplicationFiled: March 30, 2015Publication date: June 22, 2017Applicant: Novozymes A/SInventors: Poul Erik Pedersen, Jon Martin Persson, Esben Peter Friis, Simon Glanville
-
Publication number: 20170114091Abstract: A method for purifying a protein product prepared in a fermentation process where the protein of interest is present in solid, crystalline or amorphous form in the fermentation container is disclosed, where the pH of the fermentation broth is adjusted to a low pH whereby the protein of interest dissolves and can be efficiently separated from insoluble.Type: ApplicationFiled: March 30, 2015Publication date: April 27, 2017Applicant: NOVOZYMES A/SInventors: Poul Erik Pedersen, Jon Martin Persson, Esben Peter Friis
-
Publication number: 20160122772Abstract: The present invention relates to a method of producing a natively non-secreted polypeptide without a secretion signal in a Bacillus host cell and recovering the polypeptide without performing a lysis step as well as to a Bacillus host cell comprising one or more exogenous or heterologous polynucleotides encoding a natively non-secreted polypeptide with no secretion signal.Type: ApplicationFiled: June 23, 2014Publication date: May 5, 2016Applicant: Novozymes A/SInventors: Niels Banke, Michael Dolberg Rasmussen, Poul Erik Pedersen, Martin Simon Borchert
-
Patent number: 8871441Abstract: The invention provides methods of screening a microbial host cell for a property of interest in a microfluidic device, the method comprising the steps of: a) transforming a d-alanine racemase-deficient microbial host cell with a polynucleotide construct comprising: i) one or more polynucleotide region providing the property of interest when present in the host cell, and ii) at least one polynucleotide region complementing the d-alanine racemase deficiency when present in the host cell; and b) screening the transformed host cell for the property of interest in the microfluidic device in the absence of externally provided d-alanine.Type: GrantFiled: August 14, 2009Date of Patent: October 28, 2014Assignee: Novozymes A/SInventors: Thomas Lenhard, Mads Eskelund Bjoernvad, Poul Erik Pedersen
-
Publication number: 20140127782Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: ApplicationFiled: January 8, 2014Publication date: May 8, 2014Applicant: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
-
Publication number: 20140106457Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.Type: ApplicationFiled: September 23, 2013Publication date: April 17, 2014Inventors: Steen Troels Jorgensen, Poul Erik Pedersen
-
Patent number: 8658410Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: October 24, 2012Date of Patent: February 25, 2014Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
-
Publication number: 20130224757Abstract: The present invention relates to a method of screening for an improved enzyme variant, said method comprising the steps of: (i) providing a recombinant host cell capable of sporulating comprising a polynucleotide encoding a sporulation factor and a polynucleotide encoding an enzyme variant which are operably linked to an inducible promoter; (ii) culturing the host cell under conditions suitable to induce the formation of spores; (iii) culturing the spores obtained in step (ii) in a medium containing a substrate for the enzyme variant; and (iv) determining the activity of the enzyme variant.Type: ApplicationFiled: August 18, 2011Publication date: August 29, 2013Applicant: NOVOZYMES A/SInventors: Mads Bjoernvad, Poul Erik Pedersen, Marco Malten
-
Publication number: 20130045524Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: ApplicationFiled: October 24, 2012Publication date: February 21, 2013Applicant: NOVOZYMES A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
-
Patent number: 8318470Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: January 5, 2012Date of Patent: November 27, 2012Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
-
Patent number: 8232087Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: February 9, 2011Date of Patent: July 31, 2012Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
-
Publication number: 20120101019Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: ApplicationFiled: January 5, 2012Publication date: April 26, 2012Applicant: NOVOZYMES A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
-
Patent number: 7993900Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: August 16, 2010Date of Patent: August 9, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup