Patents by Inventor Pramod K. Srivastava

Pramod K. Srivastava has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6130087
    Abstract: The present invention provides methods for generating antigen-reactive cytotoxic T cells in vitro comprising culturing immune cells and antigenic cells that have at least one MHC allele in common (and preferably, are syngeneic), in which the antigenic cells have been treated according to the methods of the invention. The antigenic cells are treated by subjecting them to osmotic shock followed by irradiation. As a result, a subset of T cells are activated and mature into antigen-reactive cytotoxic T cells. The effectiveness of the procedure may be enhanced by repeated restimulations and/or the addition of heat shock protein-peptide complexes. Methods and compositions are also disclosed for the treatment and prevention in a subject of cancer or infectious disease comprising administering to the subject matched cytotoxic T cells that are generated in vitro by the present methods.
    Type: Grant
    Filed: October 7, 1996
    Date of Patent: October 10, 2000
    Assignee: Fordham University
    Inventors: Pramod K. Srivastava, Robert Binder, Nathalie E. Blachere
  • Patent number: 6048530
    Abstract: Disclosed is a family of vaccines that contain stress protein-peptide complexes which when administered to a mammal are operative to initiate in the mammal a cytotoxic T cell response against cells infected with a preselected intracellular pathogen. Also disclosed are methodologies for preparing and administering vaccines containing such stress protein-peptide complexes.
    Type: Grant
    Filed: June 19, 1997
    Date of Patent: April 11, 2000
    Assignee: Mount Sinai School of Medicine of New York University
    Inventor: Pramod K. Srivastava
  • Patent number: 6030618
    Abstract: The present invention relates to immunogenic complexes of heat shock proteins (hsp) noncovalently bound to exogenous antigenic molecules which when administered to an individual elicit specific immunological responses in the host. Methods of prevention and treatment of cancer and infectious disease are provided.
    Type: Grant
    Filed: September 10, 1996
    Date of Patent: February 29, 2000
    Assignee: Fordham University
    Inventor: Pramod K. Srivastava
  • Patent number: 6017540
    Abstract: The present invention relates to methods and compositions for eliciting an immune response and the prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases. The methods of the invention comprise administering a composition comprising an effective amount of a complex, in which the complex consists essentially of a heat shock protein (hsp) noncovalently bound to an antigenic molecule. Optionally, the methods further comprise administering antigen presenting cells sensitized with complexes of hsps noncovalently bound to an antigenic molecule. "Antigenic molecule" as used herein refers to the peptides with which the hsps are endogenously associated in vivo as well as exogenous antigens/immunogens (i.e., with which the hsps are not complexed in vivo) or antigenic/immunogenic fragments and derivatives thereof. In a preferred embodiment, the complex is autologous to the individual. In a specific embodiment, the effective amounts of the complex are in the range of 0.1 to 9.
    Type: Grant
    Filed: February 7, 1997
    Date of Patent: January 25, 2000
    Assignee: Fordham University
    Inventors: Pramod K. Srivastava, Rajiv Y. Chandawarkar
  • Patent number: 6017544
    Abstract: Disclosed is a method for inhibiting the proliferation of a tumor in a mammal. The method involves the steps of (a) isolating a stress protein-peptide complex from tumor cells previously removed from the mammal and (b) administering the isolated stress protein-peptide complex along with a cytokine to the mammal in order to stimulate in the mammal an immune response against the tumor from which the complex was isolated. Stress protein-peptide complexes having particular utility in the practice of the instant invention include the Hsp70-peptide, Hsp90-peptide and gp96-peptide complexes.
    Type: Grant
    Filed: April 16, 1998
    Date of Patent: January 25, 2000
    Assignee: Mount Sinai School of Medicine of the University of New York
    Inventor: Pramod K. Srivastava
  • Patent number: 6007821
    Abstract: The invention relates to methods and compositions for the treatment of autoimmune disease. Specifically, compositions comprising heat shock proteins, including gp96, hsp90, and hsp70, are disclosed. Immunotherapeutic methods for administering the hsp-containing compositions are disclosed. Furthermore, methods for preventing rejection of organs transplanted to treat autoimmune disease are disclosed. The disclosed methods are useful for treating a variety of autoimmune diseases, including insulin dependent diabetes mellitus.
    Type: Grant
    Filed: October 16, 1997
    Date of Patent: December 28, 1999
    Assignee: Fordham University
    Inventors: Pramod K. Srivastava, Rajiv Y. Chandawarkar
  • Patent number: 5997873
    Abstract: The present invention relates to methods of preparing a purified non-covalent heat shock protein 70-peptide complex capable of eliciting an immune response in a mammal comprising purifying heat shock protein 70-peptide complexes from mammalian tumor cells or mammalian cells infected with a virus, bacteria, or other infectious agent in the absence of ATP.
    Type: Grant
    Filed: January 13, 1994
    Date of Patent: December 7, 1999
    Assignee: Mount Sinai School of Medicine of the City University of New York
    Inventor: Pramod K. Srivastava
  • Patent number: 5985270
    Abstract: The present invention relates to methods and compositions for enhancing immunological responses and for the prevention and treatment of infectious diseases or primary and metastatic neoplastic diseases based on the administration of macrophages and/or other antigen presenting cells (APC) sensitized with heat shock proteins non-covalently bound to peptide complexes and/or antigenic components. APC are incubated in the presence of hsp-peptide complexes and/or antigenic components in vitro. The sensitized cells are reinfused into the patient with or without treatment with cytokines including but not limited to interferon-.alpha., interferon-.alpha., interleukin-2, interleukin-4, interleukin-6 and tumor neurosis factor.
    Type: Grant
    Filed: September 13, 1995
    Date of Patent: November 16, 1999
    Assignee: Fordham University
    Inventor: Pramod K. Srivastava
  • Patent number: 5961979
    Abstract: Disclosed is a family of vaccines that contain stress protein-peptide complexes which when administered to a mammal are operative at initiating in the mammal cytotoxic T cell responses against preselected intracellular pathogens. Also disclosed are methodologies for preparing and administering vaccines containing stress protein-peptide complexes.
    Type: Grant
    Filed: March 16, 1994
    Date of Patent: October 5, 1999
    Assignee: Mount Sinai School of Medicine of the City University of New York
    Inventor: Pramod K. Srivastava
  • Patent number: 5948646
    Abstract: The present invention relates to methods for preparing immunogenic, prophylactically and therapeutically effective complexes of heat shock proteins noncovalently associated with antigenic peptides of cancer cells. The claimed methods comprise the constructing of a cDNA library from cancer or preneoplastic cell RNA, expressing the cDNA library in an appropriate host cell, and recovering the immunogenic complexes from the cells. Large amounts of such immunogenic complexes can be obtained by large-scale culturing of host cells containing the cDNA library. The complexes can be used as a vaccine to elicit specific immune responses against cancer or preneoplastic cells, and to treat or prevent cancer.
    Type: Grant
    Filed: December 11, 1997
    Date of Patent: September 7, 1999
    Assignee: Fordham University
    Inventor: Pramod K. Srivastava
  • Patent number: 5935576
    Abstract: The present invention relates to immunogenic complexes of heat shock proteins (hsp) noncovalently bound to exogenous antigenic molecules which when administered to an individual elicit specific immunological responses in the host. Methods of prevention and treatment of cancer and infectious disease are provided.
    Type: Grant
    Filed: September 13, 1995
    Date of Patent: August 10, 1999
    Assignee: Fordham University
    Inventor: Pramod K. Srivastava
  • Patent number: 5837251
    Abstract: The present invention relates to methods and compositions for eliciting an immune response and the prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases. The methods of the invention comprise administering a composition comprising an effective amount of a complex, in which the complex consists essentially of a heat shock protein (hsp) noncovalently bound to an antigenic molecule. "Antigenic molecule" as used herein refers to the peptides with which the hsps are endogenously associated in vivo as well as exogenous antigens/immunogens (i.e., with which the hsps are not complexed in vivo) or antigenic/immunogenic fragments and derivatives thereof. In a preferred embodiment, the complex is autologous to the individual. The effective amounts of the complex are in the range of 10-600 micrograms for complexes comprising hsp70, 50-1000 micrograms for hsp90, and 10-600 micrograms for gp96.
    Type: Grant
    Filed: September 13, 1995
    Date of Patent: November 17, 1998
    Assignee: Fordham University
    Inventor: Pramod K. Srivastava
  • Patent number: 5830464
    Abstract: The present invention relates to methods and compositions for eliciting an immune response and the prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases. The methods of the invention comprise administering a composition comprising an effective amount of a complex, in which the complex consists essentially of a heat shock protein (hsp) noncovalently bound to an antigenic molecule in combination with administering antigen presenting cells sensitized with complexes of hsps noncovalently bound to an antigenic molecule. "Antigenic molecule" as used herein refers to the peptides with which the hsps are endogenously associated in vivo as well as exogenous antigens/immunogens (i.e., with which the hsps are not complexed in vivo) or antigenic/immunogenic fragments and derivatives thereof. In a preferred embodiment, the complex is autologous to the individual. In a specific embodiment, the effective amounts of the complex when administered intradermally are in the range of 0.
    Type: Grant
    Filed: February 7, 1997
    Date of Patent: November 3, 1998
    Assignee: Fordham University
    Inventor: Pramod K. Srivastava
  • Patent number: 5750119
    Abstract: Disclosed is a method for inhibiting the proliferation of a tumor in a mammal. The method involves the steps of (a) isolating a stress protein-peptide complex from tumor cells previously removed from the mammal and (b) administering the isolated stress protein-peptide complex back to the mammal in order to stimulate in the mammal an immune response against the tumor from which the complex was isolated. Stress protein-peptide complexes having particular utility in the practice of the instant invention include the Hsp70-peptide, Hsp90-peptide and gp96-peptide complexes.
    Type: Grant
    Filed: September 30, 1994
    Date of Patent: May 12, 1998
    Assignee: Mount Sinai School of Medicine Of The City University of New York
    Inventor: Pramod K. Srivastava
  • Patent number: 4978782
    Abstract: A novel symmetrical organic disulfide sulfonate compound is disclosed as useful as an anti-radiation agent. The compound is disodium (1,4-butylenedithio)bis(4-butanesulfinate).
    Type: Grant
    Filed: September 5, 1989
    Date of Patent: December 18, 1990
    Assignee: Vanderbilt University
    Inventors: Lamar Field, Hikmat A. Musallam, Jeffrey D. Macke, Pramod K. Srivastava
  • Patent number: 4952338
    Abstract: Unsymmetrical organic disulfide sulfinate compounds, useful as an antiradiation agents, having the general formula: ##STR1## wherein A is one or of the following polar groups, OH, COOH, SO.sub.3 Na, --C(O)--, or derivatives such as esters and nonamino nitrogen derivatives such as amides or nitriles; k is an integer of one to eight; Y is a straight or substituted aliphatic chain haivng from two to eight carbon atoms which may have one or more S--S groups interposed therebetween; m signifies the number of carbon atoms in an aliphatic chain Y containing 2 to 8 carbon stoms which may have S--S interposed; aliphatic chain Y, substituted with one or more polar groups of the number k is attached to one of the two sulfur atoms shown. The second sulfur atom is attached through an aliphatic chain of 2 to 5 carbon atoms to the sulfinate function SO.sub.2 M wherein R.sup.1 and R.sup.2 may be hydrogen, aryl, cycloalkyl or various polar groups such as OH, COOH, SO.sub.
    Type: Grant
    Filed: September 16, 1986
    Date of Patent: August 28, 1990
    Inventors: Lamar Field, Hikmat Mussallam, Jeffrey D. Macke, Pramod K. Srivastava
  • Patent number: 4943657
    Abstract: Unsymmetrical organic disulfide sulfinate compounds, useful as an antiradiation agents, having the general formula: ##STR1## wherein A is one or of the following polar groups, OH, COOH, SO.sub.3 Na, --C(O)--, or derivatives such as esters and nonamino nitrogen derivatives such as amides or nitriles; k is an integer of one to eight; Y is a straight or substituted aliphatic chain having from two to eight carbon atoms which may have one or more S--S groups interposed therebetween; m signifies the number of carbon atoms in an aliphatic chain Y containing 2 to 8 carbon atoms which may have S--S interposed; aliphatic chain Y, substituted with one or more polar groups of the number k is attached to one of the two sulfur atoms shown. The second sulfur atom is attached through an aliphatic chain of 2 to 5 carbon atoms to the sulfinate function SO.sub.2 M wherein R.sup.1 and R.sup.2 may be hydrogen, aryl, cycloalkyl or various polar groups such as OH, COOH, SO.sub.
    Type: Grant
    Filed: June 14, 1989
    Date of Patent: July 24, 1990
    Assignee: Vanderbilt University
    Inventors: Lamar Field, Hikmat A. Musallam, Jeffrey D. Macke, Pramod K. Srivastava
  • Patent number: 4883890
    Abstract: Unsymmetrical organic disulfide sulfinate compounds, useful as an antiradiation agents, having the general formula: ##STR1## wherein A is one or of the following polar groups, OH, COOH, SO.sub.3 Na, --C(O)--, or derivatives such as esters and nonamino nitrogen derivatives such as amides or nitriles; k is an integer of one to eight; Y is a straight or substituted aliphatic chain having from two to eight carbon atoms which may have one or more S--S groups interposed therebetween; m signifies the number of carbon atoms in an aliphatic chain Y containing 2 to 8 carbon atoms which may have S--S interposed; aliphatic chain Y, substituted with one or more polar groups of the number k is attached to one of the two sulfur atoms shown. The second sulfur atom is attached through an aliphatic chain of 2 to 5 carbon atoms to the sulfinate function SO.sub.2 M wherein R.sup.1 and R.sup.2 may be hydrogen, aryl, cycloalkyl or various polar groups such as OH, COOH, SO.sub.
    Type: Grant
    Filed: August 3, 1988
    Date of Patent: November 28, 1989
    Assignee: Vanderbilt University
    Inventors: Lamar Field, Hikmat A. Musallam, Jeffrey D. Macke, Pramod K. Srivastava