Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: A method of operating an augmented reality processed terrain and obstacle threat scouting service is provided. The method includes using at least one sensor of a monitoring vehicle to monitor terrain and obstacle data. The monitored terrain and obstacle data is compared with terrain and obstacle data in a terrain and obstacle database. A non-validated georeferenced delta is determined when the comparison of the monitored terrain and obstacle data with terrain and obstacle data in the terrain and obstacle database finds non-matching terrain and obstacle data. The non-validated georeferenced delta is transmitted to a ground distribution center, consumer vehicle and/or cloud based server. Non-validated georeferenced delta received at a consumer vehicle is differentiated from other terrain and obstacle data on a synthetic vision display.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases and primers.

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Abstract: Systems, methods, and devices for detecting infections in a clinical sample are provided. Small-volume clinical samples obtained at a point-of-service (POS) location and may be tested at the POS location for multiple markers for multiple diseases, including upper and lower respiratory diseases. Samples may be tested for cytokines, or for inflammation indicators. Dilution of samples, or levels of detection, may be determined by the condition or past history of a subject. Test results may be obtained within a short amount of time after sample placement in a testing device, or within a short amount of time after being obtained from the subject. A prescription for treatment of a detected disorder may be provided, and may be filled, at the POS location. A bill may be automatically generated for the testing, or for the prescription, may be automatically sent to an insurance provider, and payment may be automatically obtained.

Abstract: A method of operating an augmented reality processed terrain and obstacle threat scouting service is provided. The method includes using at least one sensor of a monitoring vehicle to monitor terrain and obstacle data. The monitored terrain and obstacle data is compared with terrain and obstacle data in a terrain and obstacle database. A non-validated georeferenced delta is determined when the comparison of the monitored terrain and obstacle data with terrain and obstacle data in the terrain and obstacle database finds non-matching terrain and obstacle data. The non-validated georeferenced delta is transmitted to a ground distribution center, consumer vehicle and/or cloud based server. Non-validated georeferenced delta received at a consumer vehicle is differentiated from other terrain and obstacle data on a synthetic vision display.

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

Abstract: A method for communicating using an architecture compatible with a multi-function control and display unit (MCDU) onboard an aircraft is provided. The method intercepts MCDU format communications transmitted by a plurality of avionics systems to the MCDU using a plurality of MCDU communication connections, by at least one processor; presents the intercepted MCDU format communications, via a graphical user interface (GUI) presented by a display device communicatively coupled to the at least one processor; receives user input data via the GUI, wherein the user input data comprises at least one of data entry, user selections, and user commands associated with the plurality of avionics systems; and transmits the user input data to the plurality of avionics systems using the plurality of MCDU communication connections, by a communication device communicatively coupled to the at least one processor.

Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases and primers.

Abstract: A method of operating an augmented reality processed terrain and obstacle threat scouting service is provided. The method includes using at least one sensor of a monitoring vehicle to monitor terrain and obstacle data. The monitored terrain and obstacle data is compared with terrain and obstacle data in a terrain and obstacle database. A non-validated georeferenced delta is determined when the comparison of the monitored terrain and obstacle data with terrain and obstacle data in the terrain and obstacle database finds non-matching terrain and obstacle data. The non-validated georeferenced delta is transmitted to a ground distribution center, consumer vehicle and/or cloud based server. Non-validated georeferenced delta received at a consumer vehicle is differentiated from other terrain and obstacle data on a synthetic vision display.

Abstract: Methods and compositions for the identification of genetic-related information are provided. At least portions of methods provided herein may be performed without thermocycling. Methods and compositions may include reagents such as nucleic acid polymerases and primers.

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Abstract: Methods and compositions for the amplification of nucleic acids are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as restriction enzymes, polymerases, ligases, primers, and polynucleotide adaptors.

Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such nucleic acid polymerases and primers.

Abstract: Systems, methods, and devices for detecting infections in a clinical sample are provided. Small-volume clinical samples obtained at a point-of-service (POS) location and may be tested at the POS location for multiple markers for multiple diseases, including upper and lower respiratory diseases. Samples may be tested for cytokines, or for inflammation indicators. Dilution of samples, or levels of detection, may be determined by the condition or past history of a subject. Test results may be obtained within a short amount of time after sample placement in a testing device, or within a short amount of time after being obtained from the subject. A prescription for treatment of a detected disorder may be provided, and may be filled, at the POS location. A bill may be automatically generated for the testing, or for the prescription, may be automatically sent to an insurance provider, and payment may be automatically obtained.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.