Patents by Inventor Pranee I. Pairs

Pranee I. Pairs has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DETERMINISTIC MECHANOPORATION FOR CELL ENGINEERING
    Publication number: 20240058382
    Abstract: Intracellular delivery of a genetic construct to immune cells including: obtaining a deterministic mechanoporation (DMP) platform that includes a substrate having a surface and a plurality of capture sites, each said capture site having a boundary shape at the surface adapted and configured to support thereon a cell, and each said capture site having a bottom and including a sub-micron-scale projection extending from the bottom toward the surface of the substrate, wherein said projection is adapted and configured to penetrate a cell membrane and/or wall of the cell, and wherein the substrate has a plurality of aspiration vias situated at the bottom of the capture sites; introducing the cells to the surface in a liquid media; capturing the cells within the capture sites by applying a first hydrodynamic force; applying a second hydrodynamic force on the captured cell and locally rupturing the membrane and/or wall of the cell with the projection, introducing the genetic construct into the cells, and releasing th
    Type: Application
    Filed: October 30, 2023
    Publication date: February 22, 2024
    Inventors: Masaru P. Rao, Harish G. Dixit, Hideaki Tsutsui, Morgan L. Dundon, Pranee I. Pairs, Stephen J. Forman, Christine E. Brown, Renate Starr, Christopher B. Ballas
  • DETERMINISTIC MECHANOPORATION FOR CELL ENGINEERING
    Publication number: 20200289568
    Abstract: Intracellular delivery of a genetic construct to immune cells including: obtaining a deterministic mechanoporation (DMP) platform that includes a substrate having a surface and a plurality of capture sites, each said capture site having a boundary shape at the surface adapted and configured to support thereon a cell, and each said capture site having a bottom and including a sub-micron-scale projection extending from the bottom toward the surface of the substrate, wherein said projection is adapted and configured to penetrate a cell membrane and/or wall of the cell, and wherein the substrate has a plurality of aspiration vias situated at the bottom of the capture sites; introducing the cells to the surface in a liquid media; capturing the cells within the capture sites by applying a first hydrodynamic force; applying a second hydrodynamic force on the captured cell and locally rupturing the membrane and/or wall of the cell with the projection, introducing the genetic construct into the cells, and releasing th
    Type: Application
    Filed: March 10, 2020
    Publication date: September 17, 2020
    Inventors: Masaru P. Rao, Harish G. Dixit, Hideaki Tsutsui, Morgan L. Dundon, Pranee I. Pairs, Stephen J. Forman, Christine E. Brown, Renate Starr, Christopher B. Ballas