Patents by Inventor Prasanna D. Khot

Prasanna D. Khot has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Broad range PCR-based compositions and methods for the detection and identification of fungal pathogens
    Patent number: 9803250
    Abstract: Disclosed herein are methods for detecting a fungal pathogen in a patient sample, involving isolating the sample, carrying out a PCR reaction on the sample to generate an amplicon that includes a region of the fungal 28S ribosomal RNA gene, and detecting the PCR amplicon. Also disclosed are sequences of primers for specifically detecting a broad range of fungal pathogens in the presence of human ribosomal DNA. In certain embodiments, the amplicon is detected by sequencing or by two-dimensional melt-curve analysis. In yet other embodiments, more than one fungal pathogen is detected in a sample using the methods disclosed herein.
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: October 31, 2017
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: David N. Fredricks, Prasanna D. Khot, Daisy L. Ko
  • Quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis
    Patent number: 8450059
    Abstract: Provided are quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis (IPA) in a patient sample, such as bronchoalveolar lavage (BAL) fluid. The methods presented herein involve isolating a patient sample, optionally extracting DNA from the sample, carrying out a quantitative PCR (qPCR) reaction on the sample to generate an amplicon that includes a region of an Aspergillus spp. ribosomal RNA (rRNA) gene, and detecting the PCR amplicon. The present disclosure also provides primers and primer sets for specifically detecting an Aspergillus spp. fungal pathogen in the presence of human ribosomal DNA (rDNA).
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: May 28, 2013
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: David N. Fredricks, Prasanna D. Khot, Daisy L. Ko
  • QUANTITATIVE PCR-BASED COMPOSITIONS AND METHODS FOR THE DIAGNOSIS OF INVASIVE PULMONARY ASPERGILLOSIS
    Publication number: 20100273169
    Abstract: Provided are quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis (IPA) in a patient sample, such as bronchoalveolar lavage (BAL) fluid. The methods presented herein involve isolating a patient sample, optionally extracting DNA from the sample, carrying out a quantitative PCR (qPCR) reaction on the sample to generate an amplicon that includes a region of an Aspergillus spp. ribosomal RNA (rRNA) gene, and detecting the PCR amplicon. The present disclosure also provides primers and primer sets for specifically detecting an Aspergillus spp. fungal pathogen in the presence of human ribosomal DNA (rDNA).
    Type: Application
    Filed: April 27, 2010
    Publication date: October 28, 2010
    Applicant: FRED HUTCHINSON CANCER RESEARCH CENTER
    Inventors: DAVID N. FREDRICKS, PRASANNA D. KHOT, DAISY L. KO
  • BROAD RANGE PCR-BASED COMPOSITIONS AND METHODS FOR THE DETECTION AND IDENTIFICATION OF FUNGAL PATHOGENS
    Publication number: 20100129821
    Abstract: Disclosed herein are methods for detecting a fungal pathogen in a patient sample, involving isolating the sample, carrying out a PCR reaction on the sample to generate an amplicon that includes a region of the fungal 28S ribosomal RNA gene, and detecting the PCR amplicon. Also disclosed are sequences of primers for specifically detecting a broad range of fungal pathogens in the presence of human ribosomal DNA. In certain embodiments, the amplicon is detected by sequencing or by two-dimensional melt-curve analysis. In yet other embodiments, more than one fungal pathogen is detected in a sample using the methods disclosed herein.
    Type: Application
    Filed: November 24, 2009
    Publication date: May 27, 2010
    Applicant: Fred Hutchinson Cancer Research Center
    Inventors: David N. Fredricks, Prasanna D. Khot, Daisy L. Ko