Abstract: Understanding the complex effects of genetic perturbations on cellular state and fitness in human pluripotent stem cells (hPSCs) has been challenging using traditional pooled screening techniques which typically rely on unidimensional phenotypic readouts. Here, Applicants use barcoded open reading frame (ORF) overexpression libraries with a coupled single-cell RNA sequencing (scRNA-seq) and fitness screening approach, a technique we call SEUSS (ScalablE fUnctional Screening by Sequencing), to establish a comprehensive assaying platform. Using this system, Applicants perturbed hPSCs with a library of developmentally critical transcription factors (TFs), and assayed the impact of TF overexpression on fitness and transcriptomic cell state across multiple media conditions. Applicants further leveraged the versatility of the ORF library approach to systematically assay mutant gene libraries and also whole gene families.

Abstract: A computing system identifies a plurality of virtualization elements executing on a cloud computing environment. The computing system retrieves a first set of data related to software resources executing across the plurality of virtualization elements. The computing system retrieves a second set of data related to hardware resources associated with the plurality of virtualization elements. The computing system retrieves delta updates to the first set of data, wherein the delta updates are retrieved in real-time. The computing system generates a first database. The first database includes the first set of data related to the software resources, the second set of data related to the hardware resources associated with the plurality of virtualization elements, and the delta updates. The computing system generates a configuration management database hosted by a configuration management database system.