Patents by Inventor Pratik Raj Singh
Pratik Raj Singh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11945840Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: GrantFiled: March 18, 2021Date of Patent: April 2, 2024Assignees: VIB VZW, Vrije Universiteit Brussel, Oxford Nanopore Technologies PLCInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
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Patent number: 11939359Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: GrantFiled: July 26, 2021Date of Patent: March 26, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Mark Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
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Patent number: 11840556Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.Type: GrantFiled: February 12, 2018Date of Patent: December 12, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh
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Publication number: 20230272017Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.Type: ApplicationFiled: October 11, 2022Publication date: August 31, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh
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Publication number: 20230079731Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: September 16, 2022Publication date: March 16, 2023Applicants: VIB VZW, VRIJE UNIVERSITEIT BRUSSEL, OXFORD NANOPORE TECHNOLOGIES PLCInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
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Patent number: 11572387Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: GrantFiled: July 2, 2018Date of Patent: February 7, 2023Assignees: VIB VZW, Vrije Universiteit Brussel, Oxford Nanopore Technologies PLCInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
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Publication number: 20220162264Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: March 18, 2021Publication date: May 26, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
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Publication number: 20220064230Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: ApplicationFiled: July 26, 2021Publication date: March 3, 2022Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, Mark Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
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Publication number: 20220056517Abstract: A system for characterising a target polynucleotide, the system comprising a membrane and a pore complex; wherein the pore complex comprises: (i) a nanopore located in the membrane, and (ii) an auxiliary protein or peptide attached to the nanopore; wherein the nanopore and the auxiliary protein or peptide together form a continuous channel across the membrane, the channel comprising a first constriction region and a second constriction region; wherein the first constriction region is formed by a portion of the nanopore, and wherein the second constriction region is formed by at least a portion of the auxiliary protein or peptide.Type: ApplicationFiled: November 7, 2019Publication date: February 24, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour, Andrew John Heron
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Publication number: 20220024985Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: July 2, 2018Publication date: January 27, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
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Publication number: 20220024994Abstract: Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.Type: ApplicationFiled: May 3, 2018Publication date: January 27, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, Han Remaut
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Publication number: 20210284696Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: March 18, 2021Publication date: September 16, 2021Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
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Patent number: 11104709Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: GrantFiled: April 6, 2017Date of Patent: August 31, 2021Assignee: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, Mark John Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
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Publication number: 20210147490Abstract: Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.Type: ApplicationFiled: May 3, 2018Publication date: May 20, 2021Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, Han Remaut
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Publication number: 20210147486Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: July 2, 2018Publication date: May 20, 2021Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
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Publication number: 20200010511Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.Type: ApplicationFiled: February 12, 2018Publication date: January 9, 2020Applicant: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh
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Publication number: 20190202876Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: ApplicationFiled: April 6, 2017Publication date: July 4, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, Mark John Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young