Abstract: The need to control the activity and fidelity of CRISPR-associated nucleases has resulted in the demand for inhibitory anti-CRISPR molecules. Current small-molecule inhibitor discovery platforms are not generalizable to multiple nuclease classes, only target the initial step in the catalytic activity, and require high concentration of nuclease, resulting in inhibitors with suboptimal attributes, including poor potency. Herein, Applicants report a high-throughput discovery pipeline consisting of a FRET-based assay that is generalizable to contemporary and emerging nucleases, operates at low nuclease concentration, and targets all catalytic steps. Applicants applied this pipeline to identify BRD7586, a cell-permeable small-molecule inhibitor of SpCas9, that is 2-fold more potent than current inhibitors. Furthermore, unlike the reported inhibitors, BRD7586 enhanced SpCas9 specificity and its activity was independent of the genomic loci, DNA repair pathway, or mode of nuclease delivery.

Abstract: An integrated end to end system and method for accident and medical emergency response are disclosed. The system includes a registration subsystem configured to register one or more entities, an emergency determining subsystem configured to determine one or more emergencies, a healthcare service selection subsystem configured to enable the one or more affected users to select one or more healthcare, a healthcare service provider connecting subsystem configured to connect the one or more healthcare service providers to the one or more affected users, a referral subsystem configured to refer the one or more affected users from one or more rural areas (a primary health centre) to a secondary health centre and from the secondary health centre to a tertiary health centre, a payment receiving subsystem configured to receive a payment from the one or more affected users via the payment card shared within a family as a family tree profile.

Abstract: A permanent magnet brushless 3-phase motor comprises windings R, Y, B, each divided into a plurality of sections 1-5 and switch means S1-S12 for selectively connecting the section of the respective winding e.g. R in series and/or parallel with all other sections of that winding R. Control means are provided for actuating the switch means S1-S12 to connect the winding sections 1-5 in different configurations while the motor is running to alter the speed/torque characteristics of the motor.

Abstract: A permanent magnet brushless 3-phase motor comprises windings R, Y, B, each divided into a plurality of sections 1-5 and switch means S1-S12 for selectively connecting the section of the respective winding e.g. R in series and/or parallel with all other sections of that winding R. Control means are provided for actuating the switch means S1-S12 to connect the winding sections 1-5 in different configurations whilst the motor is running to alter the speed/torque characteristics of the motor.