Abstract: This invention is related to nucleic acid chemistry and describes novel 1,2-dithiolane functionalized nucleoside phosphoramidites (1, Chart 1) and corresponding solid supports (2, Chart 1). In addition to these derivatives, 1,2-dithiolane moiety can also be functionalized to at the various positions of the nucleobase and sugar part as shown in Schemes 1 to 8. The nucleosides of our invention carry a primary hydroxyl for DMTr (4,4?-dimethoxytrityl) function for chain elongation. Furthermore, the phosphoramidite function is attached at the 3?-hydroxyl of the nucleoside. This allows oligonucleotide chain extension under standard DNA and RNA synthesis chemistry conditions and techniques, thus leading to high quality oligonucleotides. These derivatives are useful for introduction of reactive thiol groups either at 3?- or 5?-end of the oligonucleotides on the solid supports such as gold, silver and quantum dots.

Abstract: This invention is related to nucleic acid chemistry and describes novel 1,2-dithiolane functionalized nucleoside phosphoramidites (1, Chart 1) and corresponding solid supports (2, Chart 1). In addition to these derivatives, 1,2-dithiolane moiety can also be functionalized to at the various positions of the nucleobase and sugar part as shown in Schemes 1 to 8. The nucleosides of our invention carry a primary hydroxyl for DMTr (4,4?-dimethoxytrityl) function for chain elongation. Furthermore, the phosphoramidite function is attached at the 3?-hydroxyl of the nucleoside. This allows oligonucleotide chain extension under standard DNA and RNA synthesis chemistry conditions and techniques, thus leading to high quality oligonucleotides. These derivatives are useful for introduction of reactive thiol groups either at 3?- or 5?-end of the oligonucleotides on the solid supports such as gold, silver and quantum dots.

Abstract: The thiol modified oligonucleotides have vast number of applications in the field of nucleic acid chemistry. The conjugates generated by mono thiol groups are unstable at higher temperature, in high salt concentration buffers and in presence other thiols. There is strong need to develop a novel thiol modifier probes that can generate multiple thiol groups. Described herein are efficient processes and compounds, dithiolane phosphoramidites derivative and dithiolane succinyl supports. The advantage of our cyclic disulfide thiol modifier is multifold a) each incorporation introduces two thiol groups; b) it can be introduced at any desired site of oligonucleotides; c) The symmetrical branching nature of the spacer in the linker arm of dithiolane allows for clean oligo synthesis, where cleavage of the linker arm and thereby of loss of oligo chain is prevented.

Abstract: The thiol modified oligonucleotides have vast number of applications in the field of nucleic acid chemistry. The conjugates generated by mono thiol groups are unstable at higher temperature, in high salt concentration buffers and in presence other thiols. There is strong need to develop a novel thiol modifier probes that can generate multiple thiol groups. Described herein are efficient processes and compounds, dithiolane phosphoramidites derivative and dithiolane succinyl supports. The advantage of our cyclic disulfide thiol modifier is multifold a) each incorporation introduces two thiol groups; b) it can be introduced at any desired site of oligonucleotides; c) The symmetrical branching nature of the spacer in the linker arm of dithiolane allows for clean oligo synthesis, where cleavage of the linker arm and thereby of loss of oligo chain is prevented.

Abstract: Methods and systems to resolve the cyclic time ambiguity of a scattered pilot based channel impulse response, including to determine a channel impulse response from a combination of scattered pilots and encoded parameters, such as L1-pre signaling within P2 symbols of a terrestrial digital video broadcast (DVD) in a single frequency network (SFN), and including to re-use a corresponding window time to track the channel impulse response in the absence of encoded parameters. Methods and systems disclosed herein may be implemented with respect to channel acquisition and tracking, including adjusting a Fast Fourier Transform trigger point to reduce inter-symbol interference.

Abstract: Methods and systems to resolve the cyclic time ambiguity of a scattered pilot based channel impulse response, including to determine a channel impulse response from a combination of scattered pilots and encoded parameters, such as L1-pre signaling within P2 symbols of a terrestrial digital video broadcast (DVD) in a single frequency network (SFN), and including to re-use a corresponding window time to track the channel impulse response in the absence of encoded parameters. Methods and systems disclosed herein may be implemented with respect to channel acquisition and tracking, including adjusting a Fast Fourier Transform trigger point to reduce inter-symbol interference.