Abstract: A method for producing a biomass comprising at least 65% protein from hydrogen-oxidizing microorganisms using one or more input streams include one or more gaseous substrates and nutrient composition which are controlled and wherein the biomass is produced at a rate of more than 10 g/l/d. The produced and isolated biomass is useful as feed or to provide nutrition to one or more organisms.

Abstract: The present invention provides a method for producing a biomass comprising at least 65% protein from hydrogen-oxidising microorganisms using one or more input streams comprising one or more gaseous substrates and a nutrient composition which are controlled and wherein the biomass is produced at a rate of more than 10 g/l/d. The produced and isolated biomass is useful as feed or to provide nutrition to one or more organisms.

Abstract: The present invention provides a method for producing a biomass comprising at least 65% protein from hydrogen-oxidising microorganisms using one or more input streams comprising one or more gaseous substrates and a nutrient composition which are controlled and wherein the biomass is produced at a rate of more than 10 g/l/d. The produced and isolated biomass is useful as feed or to provide nutrition to one or more organisms.

Abstract: The present invention relates to a process for removing genetic material from a bacterial cell, specifically producing deletions in bacterial genomes or eliminating endogenous bacterial plasmids. In particular, the process relates to the transformation of bacterial cells with one or more Deletion Vectors, wherein the Deletion Vectors are capable of directing production of two or more crRNAs which target two or more PAM/Protospacers within the genomes of the bacteria within the population or within endogenous bacterial plasmids.

Abstract: The present invention relates to a process for producing and selecting for targeted mutations in bacterial genomes. In particular, the process relates to the transformation of bacteria with a Recombination Element which comprises the desired mutation followed by homologous recombination of the Recombination Element into the bacterial genome; the CRISPR/Cas system is then used to eliminate bacteria which do not have the desired mutation.

Abstract: The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.

Abstract: The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.

Abstract: The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.

Abstract: The present invention relates to a process for removing genetic material from a bacterial cell, specifically producing deletions in bacterial genomes or eliminating endogenous bacterial plasmids. In particular, the process relates to the transformation of bacterial cells with one or more Deletion Vectors, wherein the Deletion Vectors are capable of directing production of two or more crRNAs which target two or more PAM/Protospacers within the genomes of the bacteria within the population or within endo-genous bacterial plasmids.

Abstract: The present invention relates to a process for producing and selecting for targeted mutations in bacterial genomes. In particular, the process relates to the transformation of bacteria with a Recombination Element which comprises the desired mutation followed by homologous recombination of the Recombination Element into the bacterial genome; the CRISPR/Cas system is then used to eliminate bacteria which do not have the desired mutation.

Abstract: The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.

Abstract: The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.

Abstract: The present invention relates to methods of making solvents, such as butanol, acetone or ethanol. In particular, the invention relates to a process for producing a solvent, comprising the step of culturing a solventogenic Clostridium sp. in a culture medium in a culture vessel in the presence of a polysaccharide, wherein the Clostridium sp. is one which is capable of producing a CGTase, and wherein the polysaccharide is one which is a substrate for the CGTase, and harvesting solvent from the culture medium. Preferably, the Clostridium sp. is Clostridium saccharoperbutylacetonicum N1-4(HMT) or N1-504. The invention also relates to butanol, acetone and ethanol made by such a process.