Abstract: In various embodiments alpha helical peptide-based bridges for molecular biosensing “on-chip” are disclosed. The Peptide-based bridges serve as common bridges for great diversity of biosensing applications and targets including nucleic acids, proteins, antigens, antibodies, small molecules. The primary sensor element is preferably a “molecular wire” such as an alpha-helical peptide integrated into a current monitoring circuit. The engineered peptide may contain a central conjugation site for attachment of various probe molecules including nucleic acids, proteins, antigens, antibodies. The probe-containing bridge empowers the sensor to detect interactions with specific target molecules.