Abstract: A fluorescent single chain antibody construct is provided comprising a single chain antibody reactive with a target analyte and conjugated with a fluorophore. Also provided is a method for rapid and accurate detection of analytes using the fluorescent single chain antibody construct thereof. Further provided is a diagnostic kit for detecting pathogens using a fluorescent single chain antibody construct comprising a single chain antibody reactive with pathogen-related antigens and conjugated with a fluorophore.

Abstract: This invention particularly discloses an improved immunoassay method for the specific detection of anti-rubella IgM antibodies. This method employs rubella antigens comprising rubella E1 and E2 envelope glycoproteins substantially free of capsid protein. Use of this antigen composition reduces or eliminates nonspecific protein-protein interactions leading to false positive results. A sample diluent comprising urea can also be used in the process to further reduce the occurrence of nonspecific protein-protein interactions. A diagnostic kit to aid in the detection of anti-rubella IgM antibodies is also disclosed.

Abstract: This invention particularly discloses an improved immunoassay method for the specific detection of anti-rubella IgM antibodies. This method employs rubella antigens comprising rubella E1 and E2 envelope glycoproteins substantially free of capsid protein. Use of this antigen composition reduces or eliminates nonspecific protein-protein interactions leading to false positive results. A sample diluent comprising urea can also be used in the process to further reduce the occurrence of nonspecific protein-protein interactions. A diagnostic kit to aid in the detection of anti-rubella IgM antibodies is also disclosed.

Abstract: This invention particularly discloses an improved immunoassay method for the specific detection of anti-rubella IgM antibodies. This method employs rubella antigens comprising rubella E1 and E2 envelope glycoproteins substantially free of capsid protein. Use of this antigen composition reduces or eliminates nonspecific protein-protein interactions leading to false positive results. A sample diluent comprising urea can also be used in the process to further reduce the occurrence of nonspecific protein-protein interactions. A diagnostic kit to aid in the detection of anti-rubella IgM antibodies is also disclosed.

Abstract: This invention particularly discloses an improved immunoassay method for the specific detection of anti-rubella IgM antibodies. This method employs rubella antigens comprising rubella E1 and E2 envelope glycoproteins substantially free of capsid protein. Use of this antigen composition reduces or eliminates nonspecific protein-protein interactions leading to false positive results. A sample diluent comprising urea can also be used in the process to further reduce the occurrence of nonspecific protein-protein interactions. A diagnostic kit to aid in the detection of anti-rubella IgM antibodies is also disclosed.

Abstract: Recovery of antigen from cells containing an intracellular parasite, in particular a virus, by extracting the antigen from the cells with a hypertonic salt solution. CMV antigen extracted in this manner may be supported on particles and used in an agglutination assay for CMV antibody.

Abstract: A latex agglutination assay to detect antibodies against pseudorabies virus is provided. The test assays swine serum or plasma for the presence of pseudorabies virus antibody which is indicative of an acute or previous infection or vaccination. The latex reagent is a suspension of latex particles, 0.9 microns in diameter, that have adsorbed thereon antigens from disrupted and solubilized pseudorabies virus. When this material is mixed by rotation with serum containing pseudorabies antibodies, the latex will agglutinate forming visible clumps. In the absence of antibody, the latex suspension will remain smooth and evenly dispersed.

Abstract: An agglutination immunoassay for antibody wherein antigen for sensitizing a solid particulate support is treated with detergent. The resultant antigen-sensitized support is resistant to self-agglutination.

Abstract: A solid support is sensitized with soluble rubella virus antigen which is obtained by disruption and solubilization of whole (intact) rubella virus. The sensitized support is useful in an assay for rubella virus antibody.