Patents by Inventor Punam Malik

Punam Malik has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250034591
    Abstract: Methods and compositions disclosed herein generally relates to methods of determining minimum hematopoietic stem cell (HSC) chimerism and gene dosage for correction of a hematopoietic disease; in particular, in in vivo models. The invention also relates to modified lentiviral expression vectors for increase a viral titer and various methods for increasing such titers as well as expression vectors capable of enhancing such titers. The invention also relates to CHS4 chromatin insulator-derived functional insulator sequences. The invention further relates to methods for genetic correction of diseases or reducing symptoms thereof, such as sickle cell anemia, a lysosomal storage disease. The invention further relates to a method of improving and/or correcting one or more central nervous system (CNS) abnormalities caused by one or more lysosomal storage disease.
    Type: Application
    Filed: June 7, 2024
    Publication date: January 30, 2025
    Inventor: Punam Malik
  • Publication number: 20240293466
    Abstract: Compositions and methods for delivering a protein of interest such as a cell homing molecule (e.g., CXCR4) into host cells (e.g., to hematopoietic cells). The compositions and methods provided herein may be used to enhance homing and long-term engraftment of hematopoietic cells post transplantation.
    Type: Application
    Filed: July 12, 2022
    Publication date: September 5, 2024
    Inventor: Punam MALIK
  • Patent number: 11970707
    Abstract: A strong insulator fragment from foamy virus, which can be used to insulate expression of a transgene and reduce genotoxicity of integrating vectors comprising such. The insulator fragment can also be used in gene targeting constructs in gene editing.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: April 30, 2024
    Assignee: CHILDREN'S HOSPITAL MEDICAL CENTER
    Inventor: Punam Malik
  • Patent number: 11518981
    Abstract: Provided herein are methods for preparing hematopoietic stem cells (HSCs) having enhanced engraftment activity, for example, by contacting HSCs in the presence of a p38 MAPK inhibitor and a HIF-1a stabilizer.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: December 6, 2022
    Assignee: CHILDREN'S HOSPITAL MEDICAL CENTER
    Inventor: Punam Malik
  • Publication number: 20220243224
    Abstract: Methods of producing and manufacturing retroviral particles. Such methods may involve the use of an ion-exchange column with an elution buffer comprising one or more salts, wherein the elution buffer has a low total salt concentration (e.g., 400 mM to 800 mM) relative to conventional practice. In some embodiments, the retroviral particles can be generated by host cells transfected with retroviral vectors using polyethylenimine (PEI).
    Type: Application
    Filed: April 15, 2020
    Publication date: August 4, 2022
    Inventors: Punam MALIK, William SWANEY
  • Patent number: 11345740
    Abstract: Methods and compositions disclosed herein generally relates to methods of determining minimum hematopoietic stem cell (HSC) chimerism and gene dosage for correction of a hematopoietic disease; in particular, in in vivo models. The invention also relates to modified lentiviral expression vectors for increasing a viral titer and various methods for increasing such titers as well as expression vectors capable of enhancing such titers. The invention also relates to CHS4 chromatin insulator-derived functional insulator sequences. The invention also relates to methods for genetic correction of diseases or reducing symptoms thereof, such as sickle cell anemia or ?-thalassemia.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: May 31, 2022
    Assignee: Children's Hospital Medical Center
    Inventor: Punam Malik
  • Publication number: 20220056477
    Abstract: Disclosed are methods of preparing FV vector particles. In some aspects, the disclosed methods may include the steps of transfecting a population of eukaryotic cells by contacting said population of eukaryotic cells with one or more transfection reagents to form a transfection mixture, and incubating the transfection mixture to form a transfected cell population; harvesting the FV vector particles from said transfected cell population; purifying the FV vector particles; and concentrating the FV vector particles.
    Type: Application
    Filed: June 11, 2021
    Publication date: February 24, 2022
    Inventors: Johannes C.M. van der Loo, David William Russell, Punam Malik, Md Nasimuzzaman
  • Publication number: 20210380946
    Abstract: A method of conditioning a subject for hematopoietic cell transplantation, wherein the method involves the use of a nitrogen mustard alkylating agent such as melphalan in an amount to achieve reduced-intensity conditioning.
    Type: Application
    Filed: November 1, 2018
    Publication date: December 9, 2021
    Inventor: Punam Malik
  • Patent number: 11066678
    Abstract: The invention relates to methods of improving titer in transfection-based bioreactor culture production or transfection-based production systems using eukaryotic cells.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: July 20, 2021
    Assignee: Children's Hospital Medical Center
    Inventors: Johannes Van Der Loo, Punam Malik
  • Patent number: 10889646
    Abstract: Provided herein are compositions and methods related to conditioning a subject for a hematopoietic stem cell (HSC) transplantation using an inhibitor of a stem cell growth factor receptor (KIT). The compositions and methods described herein are particularly useful for a subject who is in need of a HSC transplantation and who is hypersensitive to at least one DNA damaging agent, e.g., a subject with fanconi anemia. Compositions and methods related to conditioning a fanconi anemia subject for HSC transplantation using an inhibitor of KIT are also provided herein.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: January 12, 2021
    Assignee: CHILDREN'S HOSPITAL MEDICAL CENTER
    Inventors: Punam Malik, Shanmuganathan Chandrakasan
  • Publication number: 20200255860
    Abstract: A strong insulator fragment from foamy virus, which can be used to insulate expression of a transgene and reduce genotoxicity of integrating vectors comprising such. The insulator fragment can also be used in gene targeting constructs in gene editing.
    Type: Application
    Filed: September 18, 2018
    Publication date: August 13, 2020
    Applicant: CHILDREN'S HOSPITAL MEDICAL CENTER
    Inventor: Punam MALIK
  • Publication number: 20200149065
    Abstract: Disclosed are methods of preparing FV vector particles. In some aspects, the disclosed methods may include the steps of transfecting a population of eukaryotic cells by contacting said population of eukaryotic cells with one or more transfection reagents to form a transfection mixture, and incubating the transfection mixture to form a transfected cell population; harvesting the FV vector particles from said transfected cell population; purifying the FV vector particles; and concentrating the FV vector particles.
    Type: Application
    Filed: September 6, 2019
    Publication date: May 14, 2020
    Inventors: Johannes C.M. van der Loo, David William Russell, Punam Malik, Md Nasimuzzaman
  • Publication number: 20190338249
    Abstract: Provided herein are methods for preparing hematopoietic stem cells (HSCs) having enhanced engraftment activity, for example, by contacting HSCs in the presence of a p38 MAPK inhibitor and a HIF-1a stabilizer.
    Type: Application
    Filed: January 28, 2018
    Publication date: November 7, 2019
    Applicant: CHILDREN'S HOSPITAL MEDICAL CENTER
    Inventor: Punam MALIK
  • Publication number: 20190276844
    Abstract: Methods and compositions disclosed herein generally relates to methods of determining minimum hematopoietic stem cell (HSC) chimerism and gene dosage for correction of a hematopoietic disease; in particular, in in vivo models. The invention also relates to modified lentiviral expression vectors for increase a viral titer and various methods for increasing such titers as well as expression vectors capable of enhancing such titers. The invention also relates to CHS4 chromatin insulator-derived functional insulator sequences. The invention further relates to methods for genetic correction of diseases or reducing symptoms thereof, such as sickle cell anemia, a lysosomal storage disease. The invention futher relates to a method of improving and/or correcting one or more central nervous system (CNS) abnormalities caused by one or more lysosomal storage disease.
    Type: Application
    Filed: November 20, 2018
    Publication date: September 12, 2019
    Applicant: Children's Hospital Medical Center
    Inventor: Punam Malik
  • Publication number: 20180346552
    Abstract: Methods and compositions disclosed herein generally relates to methods of determining minimum hematopoietic stem cell (HSC) chimerism and gene dosage for correction of a hematopoietic disease; in particular, in in vivo models. The invention also relates to modified lentiviral expression vectors for increasing a viral titer and various methods for increasing such titers as well as expression vectors capable of enhancing such titers. The invention also relates to CHS4 chromatin insulator-derived functional insulator sequences. The invention also relates to methods for genetic correction of diseases or reducing symptoms thereof, such as sickle cell anemia or ?-thalassemia.
    Type: Application
    Filed: June 1, 2018
    Publication date: December 6, 2018
    Applicant: Children's Hospital Medical Center
    Inventor: Punam Malik
  • Publication number: 20180325947
    Abstract: Provided herein are methods for preparing hematopoietic stem cells (HSCs), for example, human HSCs suitable for engraftment in the presence of a p38 MAPK inhibitor. Methods for assessing engraftment of HSCs (e.g., human HSCs) are also provided.
    Type: Application
    Filed: October 27, 2016
    Publication date: November 15, 2018
    Applicant: Children's Hospital Medical Center
    Inventors: Punam Malik, Marie-Dominique Filippi
  • Patent number: 10072067
    Abstract: Methods and compositions disclosed herein generally relates to methods of determining minimum hematopoietic stem cell (HSC) chimerism and gene dosage for correction of a hematopoietic disease; in particular, in in vivo models. The invention also relates to modified lentiviral expression vectors for increasing a viral titer and various methods for increasing such titers as well as expression vectors capable of enhancing such titers. The invention also relates to CHS4 chromatin insulator-derived functional insulator sequences. The invention also relates to methods for genetic correction of diseases or reducing symptoms thereof, such as sickle cell anemia or ?-thalassemia.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: September 11, 2018
    Assignee: Children's Hospital Medical Center
    Inventor: Punam Malik
  • Publication number: 20180127497
    Abstract: Provided herein are compositions and methods related to conditioning a subject for a hematopoietic stem cell (HSC) transplantation using an inhibitor of a stem cell growth factor receptor (KIT). The compositions and methods described herein are particularly useful for a subject who is in need of a HSC transplantation and who is hypersensitive to at least one DNA damaging agent, e.g., a subject with fanconi anemia. Compositions and methods related to conditioning a fanconi anemia subject for HSC transplantation using an inhibitor of KIT are also provided herein.
    Type: Application
    Filed: March 25, 2016
    Publication date: May 10, 2018
    Applicant: Children's Hospital Medical Center
    Inventors: Punam Malik, Shanmuganathan Chandrakasan
  • Publication number: 20170266164
    Abstract: This invention relates to a new use of compounds that are angiotensin II receptor agonists, specifically agonists of the angiotensin II type 2 receptor (the AT2 receptor), and especially agonists that bind selectively to the AT2 receptor, in the treatment of sickle cell disease.
    Type: Application
    Filed: December 11, 2015
    Publication date: September 21, 2017
    Inventor: Punam Malik
  • Publication number: 20170145077
    Abstract: Methods and compositions disclosed herein generally relates to methods of determining minimum hematopoietic stem cell (HSC) chimerism and gene dosage for correction of a hematopoietic disease; in particular, in in vivo models. The invention also relates to modified lentiviral expression vectors for increasing a viral titer and various methods for increasing such titers as well as expression vectors capable of enhancing such titers. The invention also relates to CHS4 chromatin insulator-derived functional insulator sequences. The invention also relates to methods for genetic correction of diseases or reducing symptoms thereof, such as sickle cell anemia or ?-thalassemia.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 25, 2017
    Applicant: Children's Hospital Medical Center
    Inventor: Punam MALIK