Patents by Inventor Pushkar Shrestha
Pushkar Shrestha has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20260201267Abstract: The present invention relates to extracted plant lipid or microbial lipid comprising docosapentaenoic acid, and processes for producing the extracted lipid.Type: ApplicationFiled: November 20, 2025Publication date: July 16, 2026Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corporation, Nuseed Nutritional Australia Pty LtdInventors: James Robertson Petrie, Surinder Pal Singh, Pushkar Shrestha, Jason Timothy McAllister, Robert Charles de Feyter, Malcolm David Devine
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Publication number: 20260182614Abstract: The present invention provides a method of producing a composition capable of producing a food-like aroma and/or flavour when heated, comprising a) disrupting a Mortierella spp. biomass comprising phospholipids; b) fractionating the disrupted biomass and collecting the supernatant therefrom; and c) combining the supernatant with: (i) one or more sugars, sugar alcohols, sugar acids, or sugar derivatives; and (ii) one or more amino acids or derivatives or salts thereof, or a compound comprising an amino group (e.g. thiamine). The present invention further provides compositions comprising a supernatant of a fractionated, disrupted Mortierella spp. biomass comprising phospholipids and methods of producing food-like aromas and/or flavours.Type: ApplicationFiled: May 12, 2023Publication date: July 2, 2026Inventors: James Robertson Petrie, Surinder Pal Singh, Anna El Tahchy, Pushkar Shrestha, Rosangela Aparecida Devilla, Hanh Nguyen
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Publication number: 20260150870Abstract: The present invention provides compositions comprising microbial biomass, and food products, beverage products or feedstuffs comprising microbial biomass. Described herein are the uses of said compositions and food products, beverage products or feedstuffs for producing food-like aromas and/or flavours when heated, in particular for undergoing Maillard reactions. Also provided are methods for producing food-like aromas and/or flavours.Type: ApplicationFiled: May 12, 2023Publication date: June 4, 2026Inventors: James Robertson Petrie, Surinder Pal Singh, Anna El Tahchy, Pushkar Shrestha, Rosangela Aparecida Devilla, Hanh Nguyen, Heinrich Kroukamp
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Patent number: 12637408Abstract: The present invention relates to lipid comprising docosapentaenoic acid and/or docosahexaenoic acid wherein the docosapentaenoic acid and/or docosahexaenoic acid may be preferentially esterified at the sn-2 position of triacylglycerol, and processes for producing and using the lipid.Type: GrantFiled: June 14, 2023Date of Patent: May 26, 2026Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATION, NUSEED NUTRITIONAL AUSTRALIA PTY LTD.Inventors: James Robertson Petrie, Surinder Pal Singh, Pushkar Shrestha, Jason Timothy McAllister, Robert Charles de Feyter, Malcolm David Devine, Xue-Rong Zhou
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Publication number: 20260132353Abstract: The present invention relates to extracted plant lipid or microbial lipid comprising docosapentaenoic acid, and processes for producing the extracted lipid.Type: ApplicationFiled: January 13, 2026Publication date: May 14, 2026Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corporation, Nuseed Nutritional Australia Pty LtdInventors: James Robertson Petrie, Surinder Pal Singh, Pushkar Shrestha, Jason Timothy McAllister, Robert Charles de Feyter, Malcolm David Devine
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Patent number: 12606853Abstract: The present invention relates to microbial cells comprising triacylglycerol (TAG) with short chain fatty acids (SCFA), as well as methods of using these cells to produce lipid comprising TAG with SCFAs.Type: GrantFiled: March 12, 2021Date of Patent: April 21, 2026Assignee: Nourish Ingredients PTY LTDInventors: Anna El Tahchy, Dawar Hussain, Surinder Pal Singh, Pushkar Shrestha, Rosangela Aparecida Devilla, James Robertson Petrie
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Patent number: 12529007Abstract: The present invention relates to extracted plant lipid or microbial lipid comprising docosapentaenoic acid, and processes for producing the extracted lipid.Type: GrantFiled: March 23, 2023Date of Patent: January 20, 2026Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATION, NUSEED NUTRITIONAL PTY LTD.Inventors: James Robertson Petrie, Surinder Pai Singh, Pushkar Shrestha, Jason Timothy McAllister, Robert Charles de Feyter, Malcolm David Devine
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Patent number: 12495751Abstract: The present embodiments provide a novel DPA-producing Brassica juncea (DPA juncea) line designated as NUBJ1207. The embodiments also provide the seeds, the plants, and the plant parts (including DNA) of DPA juncea line NUBJ1207, as well as to methods for producing a Brassica plant produced by crossing DPA juncea line NUBJ1207 with itself or another Brassica line. These embodiments also provide methods for producing a DPA-producing Brassica plant containing in its genetic material one or more transgenes, and to the transgenic plants and plant parts produced by those methods. These embodiments further provide DPA production lines or breeding lines and plant parts derived from DPA juncea line NUBJ1207, to methods for producing canola lines or plant parts derived from line NUBJ1207, and to the DPA-producing Brassica plants, varieties, and their parts derived from use of those methods.Type: GrantFiled: August 26, 2020Date of Patent: December 16, 2025Assignee: Nuseed Nutritional Australia Pty Ltd.Inventors: Greg Buzza, Srinivas Belide, Malcolm Devine, Wenxiang Gao, Nelson Gororo, Antonio Leonforte, Jason McAllister, James Petrie, Pushkar Shrestha, Surinder Singh, Xue-Rong Zhou
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Patent number: 12492354Abstract: The present invention relates to extracted plant lipid or microbial lipid comprising docosapentaenoic acid, and processes for producing the extracted lipid.Type: GrantFiled: March 30, 2023Date of Patent: December 9, 2025Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATION, NUSEED NUTRITIONAL AUSTRALIA PTY LTDInventors: James Robertson Petrie, Surinder Pai Singh, Pushkar Shrestha, Jason Timothy McAllister, Robert Charles de Feyter
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Publication number: 20250268292Abstract: The present invention relates to extracted microbial lipids, microbial cells comprising the lipid, and extracts thereof. The present invention also relates to use of these lipids, cells and extracts in foods, feedstuffs and beverages.Type: ApplicationFiled: March 3, 2022Publication date: August 28, 2025Applicants: Nourish Ingredients Pty Ltd, Nourish Ingredients Pty LtdInventors: Anna EL TAHCHY, Dawar HUSSAIN, Surinder Pal SINGH, Pushkar SHRESTHA, Rosangela Aparecida DEVILLA, Robert Charles DE FEYTER, James Robertson PETRIE, Hanh NGUYEN, Heinrich KROUKAMP
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Patent number: 12203087Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ?3 destaurases, ?5 elongases and ?6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.Type: GrantFiled: January 10, 2024Date of Patent: January 21, 2025Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATIONInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Patent number: 12195739Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ?3 destaurases, ?5 elongases and ?6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.Type: GrantFiled: January 10, 2024Date of Patent: January 14, 2025Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATIONInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Publication number: 20240407418Abstract: The present invention provides compositions comprising microbial biomass, and food products, beverage products or feedstuffs comprising microbial biomass. Described herein are the uses of said compositions and food products, beverage products or feedstuffs for producing food-like aromas and/or flavours when heated, in particular for undergoing Maillard reactions. Also provided are methods for producing food-like aromas and/or flavours.Type: ApplicationFiled: October 20, 2022Publication date: December 12, 2024Applicants: Nourish Ingredients Pty Ltd, COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATIONInventors: James Robertson PETRIE, Surinder Pal SINGH, Anna EL TAHCHY, Pushkar SHRESTHA, Rosangela Aparecida DEVILLA, Hanh NGUYEN, Heinrich KROUKAMP
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Publication number: 20240360464Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ?3 destaurases, ?5 elongases and ?6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.Type: ApplicationFiled: May 6, 2024Publication date: October 31, 2024Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development CorporationInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Publication number: 20240150781Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ?3 destaurases, ?5 elongases and ?6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.Type: ApplicationFiled: January 10, 2024Publication date: May 9, 2024Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development CorporationInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Publication number: 20240150779Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ?3 destaurases, ?5 elongases and ?6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.Type: ApplicationFiled: January 10, 2024Publication date: May 9, 2024Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development CorporationInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Publication number: 20240150780Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ?3 destaurases, ?5 elongases and ?6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.Type: ApplicationFiled: January 10, 2024Publication date: May 9, 2024Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development CorporationInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Publication number: 20240150782Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ?3 desaturases, ?5 elongases and ?6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.Type: ApplicationFiled: January 10, 2024Publication date: May 9, 2024Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development CorporationInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Patent number: 11976287Abstract: The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.Type: GrantFiled: April 28, 2020Date of Patent: May 7, 2024Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATIONInventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
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Publication number: 20230416186Abstract: The present invention relates to lipid comprising docosapentaenoic acid and/or docosahexaenoic acid wherein the docosapentaenoic acid and/or docosahexaenoic acid may be preferentially esterified at the sn-2 position of triacylglycerol, and processes for producing and using the lipid.Type: ApplicationFiled: June 14, 2023Publication date: December 28, 2023Applicants: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corporation, Nuseed Nutritional Australia Pty LtdInventors: James Robertson Petrie, Surinder Pal Singh, Pushkar Shrestha, Jason Timothy McAllister, Robert Charles de Feyter, Malcolm David Devine, Xue-Rong Zhou