Abstract: The present invention involves a device for culturing cells on removable membranes/sponges for the purpose of transplantation. The device includes a base plate with snaps and a ring, which fits into the base plate. The membrane/sponge of choice for culturing cells is placed on the base plate and fixed by the ring, which fits tightly to the base. The device is placed inside a suitable culture vessel or receptacle and the cell suspension is added within the insert device. The cells are retained on the membrane, which enables cell attachment and proliferation. Alternatively, small tissue explants can be placed on the membrane enabling cell migration and proliferation. Cells are cultured under standard conditions with regular media changes. Following shipping to the hospitals under appropriate conditions, the insert device would be dismantled and the membrane lifted and placed at the site of transplantation.

Abstract: The present invention involves a device for culturing cells on removable membranes/sponges for the purpose of transplantation. The device includes a base plate with snaps and a ring, which fits into the base plate. The membrane/sponge of choice for culturing cells is placed on the base plate and fixed by the ring, which fits tightly to the base. The device is placed inside a suitable culture vessel or receptacle and the cell suspension is added within the insert device. The cells are retained on the membrane, which enables cell attachment and proliferation. Alternatively, small tissue explants can be placed on the membrane enabling cell migration and proliferation. Cells are cultured under standard conditions with regular media changes. Following shipping to the hospitals under appropriate conditions, the insert device would be dismantled and the membrane lifted and placed at the site of transplantation.