Abstract: A method for laser distance measuring includes: emitting an incident ray ? having an incident angle ? to a reflective surface of a laser reflecting mirror (31), the incident ray ? being reflected by the laser reflecting mirror (31) to generate a first reflected ray ?, and the first reflected ray ? irradiating an object to be measured (1000); capturing a second reflected ray ? and generating a laser image on a laser imaging plane (3221), the second reflected ray ? being generated by the reflective surface of the laser reflecting mirror (31) reflecting a return ray ? generated after the first reflected ray ? irradiates a surface of the object to be measured; and determining, based on the laser image, a measurement distance A according to a geometrical trigonometry.

Abstract: The present disclosure provides a counting chamber and an application thereof, and a method and a system for analyzing particles in a test sample. The method includes obtaining a full volume image of the test sample using an image acquisition device, wherein an imaging field of the full volume image is capable of reflecting an entire volume of the test sample in a sample container. The method further includes determining an analysis parameter of the particles in the test sample based on the full volume image, wherein the analysis parameter of the particles includes at least a count of the particles.

Abstract: The embodiments of the present disclosure provide a sample analysis device and a sample analysis system. The sample analysis device includes a base and one or more sample analysis units provided on the base. Each sample analysis unit includes a liquid storage chamber, an optical imaging chamber, a drainage chamber, and a sealing element. The liquid storage chamber is arranged on the base. The optical imaging chamber is arranged on the base, and a side of the optical imaging chamber is connected to the liquid storage chamber. The drainage chamber is arranged on the base. The drainage chamber is connected to the other side of the optical imaging chamber, and the drainage chamber is provided with an opening connecting with atmosphere. The sealing element removably seals the opening.

Abstract: A method for laser distance measuring includes: emitting an incident ray ? having an incident angle ? to a reflective surface of a laser reflecting mirror (31), the incident ray ? being reflected by the laser reflecting mirror (31) to generate a first reflected ray ?, and the first reflected ray ? irradiating an object to be measured (1000); capturing a second reflected ray ? and generating a laser image on a laser imaging plane (3221), the second reflected ray ? being generated by the reflective surface of the laser reflecting mirror (31) reflecting a return ray ? generated after the first reflected ray ? irradiates a surface of the object to be measured; and determining, based on the laser image, a measurement distance A according to a geometrical trigonometry.

Abstract: The present disclosure disclose a microscopic device and a focusing method of the microscopic device, the microscopic device includes an object stage configured to carry a transparent carrier and drive the transparent carrier to translate along at least one direction, the transparent carrier includes at least two object positions; a microscopic objective located on a side of the object stage; a rangefinder located on a same side of the object stage as a microscopic objective and at a same height relative to the object stage, and the rangefinder is configured to measure a distance between a surface of each of the at least two object positions and the microscopic objective; a focusing module configured to adjust a position of the microscopic objective along a first direction based on the distance measured by the rangefinder, wherein the first direction is perpendicular to a carrying surface of the object stage.

Abstract: The embodiments of the present disclosure provide a method, a system, and an application for detecting at least one of a cell-killing efficacy or an immune activity. The method comprises: obtaining a plurality of microscopic images of a fixed area of a co-culture sample, wherein the co-culture sample is a cell sample obtained by co-culturing target cells and effector cells, the fixed area of the co-culture sample includes a plurality of objects, wherein the plurality of objects are a cell group including cells with different properties, each of the plurality of objects having an image-identifiable feature; performing an image overlapping synthesis analysis or an image fusion analysis for the plurality of microscopic images to obtain the cell properties of the plurality of objects and make statistics to cell parameters associated with the cell properties; and evaluating at least one of the cell-killing efficacy or the immune activity of the effector cells based on the cell parameters.

Abstract: A particle detection device is provided. The particle detection device may include a substrate and at least one detection unit setting on the substrate.

Abstract: The present disclosure discloses a microscopic device (100, 1400, 1900) and an image processing device (1500). The image processing device (1500) includes a receiving device (1501) configured to receive a digital image generated by the microscopic device (100, 1400, 1900), the digital image includes a scaling pattern (202, 302, 402, 502, 702, 802, 902, 1002); a storage device (1502) configured to store the digital image; and a processor (1503) configured to determine a magnification of the microscopic device (100, 1400, 1900) based on the scaling pattern (202, 302, 402, 502, 702, 802, 902, 1002).

Abstract: A method and a system for fluorescence intensity of a fluorescence image are provided. In the method, fluorescence imaging is performed on a target sample to obtain a fluorescent image. Edge extraction and segmentation is performed on each detection target in the fluorescence image, to obtain the fluorescent image area of each detection target in the fluorescence image. At least one of a cumulative gray-scale value, a maximum gray-scale value and an average gray-scale value of the fluorescence image region and a diameter value of a bright field image region of each detected target is calculated. Then, the flow clustering analysis is calculated based on at least one of the cumulative grayscale value, the maximum grayscale value, the average grayscale value, and the bright field diameter value.

Abstract: A method and a system for fluorescence intensity of a fluorescence image are provided. In the method, fluorescence imaging is performed on a target sample to obtain a fluorescent image. Edge extraction and segmentation is performed on each detection target in the fluorescence image, to obtain the fluorescent image area of each detection target in the fluorescence image. At least one of a cumulative gray-scale value, a maximum gray-scale value and an average gray-scale value of the fluorescence image region and a diameter value of a bright field image region of each detected target is calculated. Then, the flow clustering analysis is calculated based on at least one of the cumulative grayscale value, the maximum grayscale value, the average grayscale value, and the bright field diameter value.

Abstract: A fluorescence microscopic imaging method includes: after a to-be-detected sample plate is placed, lightening, according to experimental requirements, at least one monochromatic fluorescence excitation light source with a same color among multiple monochromatic fluorescence excitation light sources as a target light source, where monochromatic fluorescence excitation light emitted by each monochromatic fluorescence excitation light source obliquely enters a preset detection region of the to-be-detected sample plate; collecting, at a side of the to-be-detected sample plate facing away from the target light source, fluorescence of particles within the preset detection region excited by irradiation of monochromatic fluorescence excitation light emitted by the target light source, and magnifying the preset detection region a preset number of times; filtering the excited fluorescence of the particles within the preset detection region; and acquiring a fluorescence image of the preset detection region.

Abstract: A fluorescence microscopic imaging method includes: after a to-be-detected sample plate is placed, lightening, according to experimental requirements, at least one monochromatic fluorescence excitation light source with a same color among multiple monochromatic fluorescence excitation light sources as a target light source, where monochromatic fluorescence excitation light emitted by each monochromatic fluorescence excitation light source obliquely enters a preset detection region of the to-be-detected sample plate; collecting, at a side of the to-be-detected sample plate facing away from the target light source, fluorescence of particles within the preset detection region excited by irradiation of monochromatic fluorescence excitation light emitted by the target light source, and magnifying the preset detection region a preset number of times; filtering the excited fluorescence of the particles within the preset detection region; and acquiring a fluorescence image of the preset detection region.

Abstract: A follow-up fixed focus system includes a fixed focus device, and the second end of the fixed focus device is in contact with a sample plate; when the sample plate has a small protrusion, the second end of the fixed focus device is pushed by the protrusion of the sample plate. When the sample plate has a small depression, the second end of the fixed focus device is in contact with the sample plate all the time under the action of self gravity of an observation device, so that the imaging distance between an object lens and the sample plate is kept unchanged.

Abstract: A follow-up fixed focus system includes a fixed focus device, and the second end of the fixed focus device is in contact with a sample plate; when the sample plate has a small protrusion, the second end of the fixed focus device is pushed by the protrusion of the sample plate. When the sample plate has a small depression, the second end of the fixed focus device is in contact with the sample plate all the time under the action of self gravity of an observation device, so that the imaging distance between an object lens and the sample plate is kept unchanged.