Abstract: A method for detecting a whole transcriptome RNA structure and the use thereof A method for detecting an intact RNA structure by combining in vivo click chemistry selective 2?-hydroxy acylation and mutation map analysis. Combined with the RNA immunoprecipitation technology, the method is further applied to analyze a RNA structure map of Dicer-bound substrates, and reveals the structural type and characteristics of the Dicer substrates. The method provided for detecting a whole transcriptome RNA structure can also perform complete full-length structure analysis on small RNAs, thereby laying the foundation for the research on the structure and biological functions of RNA molecules in a whole transcriptome in cells.

Abstract: Provided in the present invention are a method for detecting an RNA structure and the use thereof. According to the present invention, the step of removing the background of reverse transcription termination signals is included in the method for detecting an RNA structure, and false positive signals in a structural score calculation are reduced, and therefore the accuracy of the detection method is improved.