Abstract: An application of a branched-chain ?-ketoacid dehydrogenase complex in preparation of malonyl coenzyme A. A method for preparing malonyl-CoA using a branched-chain ?-ketoacid dehydrogenase complex, the method comprising introducing a gene encoding a branched-chain ?-ketoacid dehydrogenase complex into a biological cell strain to obtain a recombinant cell strain capable of expressing the gene encoding the branched-chain ?-ketoacid dehydrogenase complex; culturing the recombinant cell strain to prepare malonyl-CoA; the branched-chain ?-ketoacid dehydrogenase complex is the following M1) or M2): M1) a set of proteins consisting of a bkdF protein, a bkdG protein, a bkdH protein and a lpdA1 protein; M2) a set of proteins consisting of a bkdA protein, a bkdB protein, a bkdC protein and the lpdA1 protein.

Abstract: Provided is a method for construction of recombinant bacteria for producing 3-hydroxypropionic acid. The method includes: knocking out fadR, fabF and fabH genes of recipient bacteria, introducing acc genes or gene clusters, alKL and Mcr genes, and enhancing the expression of fadL, fadD, sthA genes and atoSC gene clusters in the recipient bacteria. Also provided is a method for producing 3-hydroxypropionic acid by using the recombinant bacteria.

Abstract: Provided is a method for construction of recombinant bacteria for producing 3-hydroxypropionic acid. The method includes: knocking out fadR, fabF and fabH genes of recipient bacteria, introducing acc genes or gene clusters, alKL and Mcr genes, and enhancing the expression of fadL, fadD, sthA genes and atoSC gene clusters in the recipient bacteria. Also provided is a method for producing 3-hydroxypropionic acid by using the recombinant bacteria.

Abstract: Embodiments of the present invention disclose a method for updating a Web App. The method includes: when receiving an update request for updating a currently running Web application, continuing to run the Web application, and downloading an update package of a new version of the Web application; after the downloading of the update package is completed, performing installation of the update package in a background, where a new installation file generated during the installation is stored in a first path, which is different from a second path where an installation file of a current version of the Web application is stored; and loading a page file in the new installation file to the Web application to display a page of the new version.