Abstract: The present disclosure belongs to the technical field of temperature detection devices for microfluidic chips, specifically relates to a temperature detection device and method in loop mediated isothermal amplification (LAMP). The temperature detection device in the LAMP includes a temperature measurement mechanism, a heat conduction pad, a heating layer, and a thermal insulation layer which are disposed in sequence from top to bottom; the temperature measurement mechanism enters a corresponding temperature measurement chamber to collect corresponding working chamber temperature data, that is, the heating layer adjusts, according to the corresponding working chamber temperature data, heat generated by a corresponding heating source; the thermal insulation layer prevents the heating layer from downwards transmitting the heat such that the heats generated by the heating sources in the heating layer are all transmitted to corresponding heating regions through the heat conduction pad.

Abstract: The present disclosure relates to a rapid genetic screening method and device. The method includes: collecting a sample to be tested of a patient through a micro-fluidic chip, where the sample to be tested includes a whole blood or saliva or nasopharyngeal swab or wound swab sample of a patient; lysing and amplifying the sample to be tested in the micro-fluidic chip to obtain an amplified nucleic acid segment; fusing a biosensor with amplification liquid, where the biosensor is provided with a DNA probe which can only be bounded to a specific nucleic acid segment and in which an impedance may dramatically change before and after the bounding; and inputting an electrical signal to the biosensor, testing a signal of an output end, and determining whether a nucleic acid segment matched with the DNA probe exists in the sample to be tested of the patient. The DNA probe can be replaced to test whether different nucleic acid segments exist.