Abstract: The present invention provides a rice whole genome breeding chip and the application thereof. The rice whole genome breeding chip of the present invention is Rice60K, an SNP chip manufactured based on Infinium technique. Each chip can detect 24 samples simultaneously and contains 58,290 SNP sites. The marker sites have DNA sequences represented by SEQ ID NO.1-58290. The chip can be used in molecular marker fingerprint analysis of the rice variety resources, in genotype identification of the hybrid progeny population, in identification of the variety authenticity, in analysis and screening of the genetic background of the breeding materials, and in association analysis of the agronomic traits, having wide application prospects.

Abstract: The present invention provides a rice whole genome breeding chip and the application thereof. The rice whole genome breeding chip of the present invention is Rice60K, an SNP chip manufactured based on Infinium technique. Each chip can detect 24 samples simultaneously and contains 58,290 SNP sites. The marker sites have DNA sequences represented by SEQ ID NO.1-58290. The chip can be used in molecular marker fingerprint analysis of the rice variety resources, in genotype identification of the hybrid progeny population, in identification of the variety authenticity, in analysis and screening of the genetic background of the breeding materials, and in association analysis of the agronomic traits, having wide application prospects.

Abstract: The present invention relates to an isolated major gene GS3 which regulates grain weight and grain length in the rice and the cloning of said gene. The DNA sequence of GS3 gene is as shown in SEQ ID NO. 1 and is 7883 bp in length. GS3 gene comprises 5 exons and encodes 232 amino acids. It is predicted based on bioinformatics analysis that said protein contains conserved domains including a PEBP-like domain, a transmembrane domain, a cysteine-rich domain of TNFR/NGFR and a VWFC domain. cDNA sequence of said gene is as shown in SEQ ID NO. 2. By sequence alignment between three large grain species and 3 small grain species of rice, it is revealed there is only one common single nucleotide mutation in a 7.9-kb region between the two different grain-length groups. Said nucleotide mutation is located at the second exon of the GS3 gene, in which a cysteine codon (TGC) in the small-grain group is mutated to a termination codon (TGA) in the large-grain group.

Abstract: The cloning and application of a pleiotropic gene Ghd7 that controls grains yield, heading date and plant height of rice are provided. The gene has the nucleotide sequence as shown in SEQ ID NO: 1. The sequence of the present gene is 3,917 bp in length, contains two exons and encodes 257 amino acids. The cDNA sequence of the gene is as shown in SEQ ID NO: 1. The present gene encodes a protein having the CCT domain of CO protein and having the amino acid sequence as shown in SEQ ID NO: 1. Rice plants transformed with GHD7 gene are obtained using transgenic technology. The transgenic plants all exhibit markedly increased yield, larger number of spikelets per panicle, delayed heading date and elevated plant height as compared to their respective controls (wild type receptors plants). The trait changes are quite consistent with the phenotypes of the two parent genotypes of GHD7 near isogenic lines of Zhenshan 97.

Abstract: The cloning and application of a pleiotropic gene Ghd7 that controls grains yield, heading date and plant height of rice are provided. The gene has the nucleotide sequence as shown in SEQ ID NO: 1. The sequence of the present gene is 3,917 bp in length, contains two exons and encodes 257 amino acids. The cDNA sequence of the gene is as shown in SEQ ID NO: 1. The present gene encodes a protein having the CCT domain of CO protein and having the amino acid sequence as shown in SEQ ID NO: 1. Rice plants transformed with GHD7 gene are obtained using transgenic technology. The transgenic plants all exhibit markedly increased yield, larger number of spikelets per panicle, delayed heading date and elevated plant height as compared to their respective controls (wild type receptors plants). The trait changes are quite consistent with the phenotypes of the two parent genotypes of GHD7 near isogenic lines of Zhenshan 97.

Abstract: The present invention relates to an isolated major gene GS3 which regulates grain weight and grain length in the rice and the cloning of said gene. The DNA sequence of GS3 gene is as shown in SEQ ID NO. 1 and is 7883 bp in length. GS3 gene comprises 5 exons and encodes 232 amino acids. It is predicted based on bioinformatics analysis that said protein contains conserved domains including a PEBP-like domain, a transmembrane domain, a cysteine-rich domain of TNFR/NGFR and a VWFC domain. cDNA sequence of said gene is as shown in SEQ ID NO. 2. By sequence alignment between three large grain species and 3 small grain species of rice, it is revealed there is only one common single nucleotide mutation in a 7.9-kb region between the two different grain-length groups. Said nucleotide mutation is located at the second exon of the GS3 gene, in which a cysteine codon (TGC) in the small-grain group is mutated to a termination codon (TGA) in the large-grain group.