Abstract: The present disclosure provides a method for ultrafastly obtaining a two-dimensional J-resolved NMR spectrum with a high-resolution against an inhomogeneous magnetic field. The method utilizes the selective excitation module and the reunion sampling module jointly, which breaks through the limitations of existing methods for obtaining the two-dimensional J-resolved NMR spectrum and effectively eliminates an influence of the inhomogeneous magnetic field along an encoding direction. At the same time, an inhomogeneous magnetic field along x and y directions is theoretically eliminated by a slow rotation of the sample. As a consequence, a two-dimensional J-resolved NMR spectrum with a high resolution is obtained by a single-scanning sampling under the inhomogeneous magnetic field, thus significantly shortening experimental duration and expanding application fields of the two-dimensional J-resolved NMR spectrum.