Abstract: In some aspects, the disclosure relates to compositions and methods for production of recombinant adeno-associated virus (rAAV) particles. The disclosure is based, in part, on isolated nucleic acids and systems that include only two vectors and are suitable for production of multiple different single gene viral vectors in a multiple small-scale campaign mode (e.g., 1013 to 1016 viral particles.

Abstract: In some aspects, the disclosure relates to compositions and methods for production of recombinant adeno-associated virus (rAAV) particles. The disclosure is based, in part, on isolated nucleic acids and systems that include only two vectors and are suitable for production of multiple different single gene viral vectors in a multiple small-scale campaign mode (e.g., 1013 to 1016 viral particles.

Abstract: The present invention relates to T cell receptors (TCRs) which bind the HLA-A2 restricted CLGGLLTMV peptide (SEQ ID NO: 1) derived from the LMP2A protein from Epstein Barr Virus (EBY). TCRs of the invention comprise a TCR alpha chain variable domain and/or a TCR beta variable domain. Certain preferred TCRs also bind the natural peptide variants SLGGLLTMV (SEQ ID NO: 17) and CLGGLITMV (SEQ ID NO: 18) presented as a peptide-HLA-A2 complex. The TCRs of the invention demonstrate excellent specificity profiles for those LMP2A epitopes and have binding affinities for the complex which result in an enhanced ability to recognize the complex compared to a soluble reference TCR having the extracellular sequence of the native EBY LMP2A TCR alpha chain given in FIG. 3 (SEQ ID No: 4) and the extracellular sequence of the native EBY LMP2A TCR beta chain given in FIG. 4 (SEQ ID No: 5).

Abstract: A system and method to measure the clock skew between transmitting and receiving devices operating with independent clock sources over a packet network is described. To provide adaptive playout in an IP telephony device without a sequencing scheme in the packets, the clock skew is measured and recorded. Using a PCM resampler that is implemented with an interpolation filter bank of FIR subfilters, the change in depth of the playout buffer during transmission is analyzed, and this change infers the clock rate associated with the transmission.

Abstract: A system and method to measure the clock skew between transmitting and receiving devices operating with independent clock sources over a packet network is described. To provide adaptive playout in an IP telephony device without a sequencing scheme in the packets, the clock skew is measured and recorded. Using a PCM resampler that is implemented with an interpolation filter bank of FIR subfilters, the change in depth of the playout buffer during transmission is analyzed, and this change infers the clock rate associated with the transmission.