Abstract: Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.

Abstract: Provided is a polynucleotide for identifying a male or female Ginkgo biloba plant, comprising or consisting of the following sequence: 1) the nucleotide sequence represented by SEQ ID NO: 5; or 2) a complementary, degenerate or homologous sequence of the sequence represented by SEQ ID NO: 5; or 3) a polynucleotide hybridizing to the nucleotide sequence represented by SEQ ID NO: 5 under stringent conditions or a complementary sequence thereof; 4) the coding sequence of any one of the sequences of the above 1)-3); or 5) a nucleotide sequence being identical to more than 10 bp contiguous nucleotides in any one of the sequences of the above 1)-4). The polynucleotide provided herein is unique to a male Ginkgo biloba plant, and it may be used to identify a male or female Ginkgo biloba plant; this identification method has the advantages of stable and accurate results, good repeatability and fast detection, thereby providing the basis for identifying a male or female Ginkgo biloba plant by molecular markers.