Abstract: A composition and method for treating cancer in a subject in need thereof. The composition includes recombinant-methioninase-producing bacterial strains that upon oral administration can release recombinant-methioninase in the gut of the subject. The bacterial strains include Escherichia coli bacteria comprising the cloned rMETase gene from Pseudomonas putida using plasmid pATG3131, such as Escherichia coli JM109 strain. The bacteria can be incorporated in the microbiome of the subject and release the recombinant-methioninase.

Abstract: A composition and method for reversing or preventing age-related metabolic and tissue degenerative changes in individuals. The method includes administering, orally, an effective amount of a first composition having methioninase enzyme. The method further includes administering, by a route other than the oral route, such as a parenteral route, an effective amount of a second composition having methioninase enzyme. The method further includes the steps of orally and/or parentally administering pyridoxal-L-phosphate.

Abstract: A composition and method for lowing serum and plasma levels of methionine by oral administration. The composition includes a recombinant methioninase enzyme and a cofactor (pyridoxal-L-phosphate). Methods of use describe methods for treatment of cancer, including malignant melanoma, by oral administration of the methioninase composition. Methods for chronic suppressive therapy of melanoma and other cancers are described. Because reduction of plasma methionine levels is effective in treating other conditions, including diabetes and conditions associated with aging, the use of the methods described herein includes treatment of these and other conditions.

Abstract: The present disclosure discloses an integrated rotary cutting tool manufacturing device and method, the integrated rotary cutting tool manufacturing device comprises a workpiece transfer device, a machine body, a feeding device, grinding devices and a discharge device. The grinding devices comprise a rough grinding device used for carrying out primary grinding on a vertically arranged workpiece and a fine grinding device used for carrying out secondary grinding on the vertically arranged workpiece. The integrated rotary cutting tool manufacturing device is adopted by the rotary cutting tool manufacturing method. The device according to the present disclosure is compact in structure, high in machining efficiency and precision, and low in costs, the maximum values, the minimum values and the average values of the roundness and the concentricity of machined micro drill bits are closer to the standard values, and the stabilization process capability indexes of the roundness.

Abstract: A composition and method for lowing serum and plasma levels of methionine by oral administration. The composition includes a recombinant methioninase enzyme and a cofactor (pyridoxal-L-phosphate). Methods of use describe methods for treatment of cancer, including malignant melanoma, by oral administration of the methioninase composition. Methods for chronic suppressive therapy of melanoma and other cancers are described. Because reduction of plasma methionine levels is effective in treating other conditions, including diabetes and conditions associated with aging, the use of the methods described herein includes treatment of these and other conditions.

Abstract: Oral composition and a method for the treatment of coronaviruses infections in humans and animals. The method includes the steps of administering orally a methionine cleaving-enzyme; administering orally or parentally a protease inhibitor; and orally administering pyridoxal-L-phosphate in a fluid.

Abstract: A composition and method for lowing serum and plasma levels of methionine by oral administration. The composition includes a recombinant methioninase enzyme and a cofactor (pyridoxal-L-phosphate). Methods of use describe methods for treatment of cancer, including malignant melanoma, by oral administration of the methioninase composition. Methods for chronic suppressive therapy of melanoma and other cancers are described. Because reduction of plasma methionine levels is effective in treating other conditions, including diabetes and conditions associated with aging, the use of the methods described herein includes treatment of these and other conditions.

Abstract: A composition and method for lowing serum and plasma levels of methionine by oral administration. The composition includes a recombinant methioninase enzyme and a cofactor (pyridoxal-L-phosphate). Methods of use describe methods for treatment of cancer, including malignant melanoma, by oral administration of the methioninase composition. Methods for chronic suppressive therapy of melanoma and other cancers are described. Because reduction of plasma methionine levels is effective in treating other conditions, including diabetes and conditions associated with aging, the use of the methods described herein includes treatment of these and other conditions.

Abstract: A composition and method for lowing serum and plasma levels of methionine by oral administration. The composition includes a recombinant methioninase enzyme and a cofactor (pyridoxal-L-phosphate). Methods of use describe methods for treatment of cancer, including malignant melanoma, by oral administration of the methioninase composition. Methods for chronic suppressive therapy of melanoma and other cancers are described. Because reduction of plasma methionine levels is effective in treating other conditions, including diabetes and conditions associated with aging, the use of the methods described herein includes treatment of these and other conditions.

Abstract: The present disclosure discloses an integrated rotary cutting tool manufacturing device and method, the integrated rotary cutting tool manufacturing device comprises a workpiece transfer device, a machine body, a feeding device, grinding devices and a discharge device. The grinding devices comprise a rough grinding device used for carrying out primary grinding on a vertically arranged workpiece and a fine grinding device used for carrying out secondary grinding on the vertically arranged workpiece. The integrated rotary cutting tool manufacturing device is adopted by the rotary cutting tool manufacturing method. The device according to the present disclosure is compact in structure, high in machining efficiency and precision, and low in costs, the maximum values, the minimum values and the average values of the roundness and the concentricity of machined micro drill bits are closer to the standard values, and the stabilization process capability indexes of the roundness.

Abstract: Infection by P. carinii can be treated by administering methioninase optionally in combination with additional therapeutic agents, such as antibiotics.

Abstract: A method to determine a total cysteine in biological fluids utilizes similarly treated portions of the fluid with a homocysteinase and a non-specific desulfurase.

Abstract: Infection by P. carinii can be treated by administering methioninase optionally in combination with additional therapeutic agents, such as antibiotics.

Abstract: A homocysteine assay that can be performed on very small quantities of blood is described. This assay is suitable for screening, including screening newborns for homocysteinuria.

Abstract: The invention provides novel methods relating to SAM detection and production as well as a novel SAHH enzymatic activity for use in such methods. Additional methods, compositions, and kits relating to the novel SAHH are also provided.

Abstract: A method to detect and measure the concentration of hydrogen peroxide comprises adding reagents consisting essentially of peroxidase and a dialkyl phenylenediamine and detecting any color generated by the interaction of hydrogen peroxide with these reagents.

Abstract: A method to determine a total cysteine in biological fluids utilizes similarly treated portions of the fluid with a homocysteinase and a non-specific desulfurase.

Abstract: The invention provides novel methods relating to SAM detection and production as well as a novel SAHH enzymatic activity for use in such methods. Additional methods, compositions, and kits relating to the novel SAHH are also provided.

Abstract: Enzymatic methods to determine the concentration of pyridoxal 5′-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular disease. The assay can be a homogeneous assay using the ability of PLP to function as a co-enzyme for homocysteinase and related enzymes and measuring the products of the reaction preferably spectrophotometrically. The invention also includes improvements in sensitivity of assays for measuring hydrogen sulfide production by measuring fluorescence as opposed to absorbance of the oxidized product of H2S with N,N-dialkyl p-phenylene diamine.

Abstract: A method to assess the level of folate in a biological sample comprises.