Abstract: Provided is a new-type cis-replicon construct for efficiently expressing a target protein, which comprises an RNA polymerase coding unit and a target protein coding unit. The replicon construct can drive the replication of the target protein coding unit and reduce or avoid the replication and expression of the RNA polymerase, thereby effectively improving the expression of the target protein and reducing the expression of the non-target protein.

Abstract: There is disclosed a reversible heat pump system 100 and a method of operating a reversible heat pump system to control the temperature of a process fluid of a chiller system 500. In a cooling mode, a working fluid is circulated for co-current flow with a process fluid at a heat exchanger 104 functioning as an evaporator heat exchanger, whereas in a heating mode, the working fluid is circulated for counter-current flow with the process fluid at the same heat exchanger 104 functioning as a condenser heat exchanger.

Abstract: Systems and methods for controlled subcooling of working fluid in a heating, ventilation, air conditioning and refrigeration (HVACR) system through a suction line heat exchanger are disclosed. The suction line heat exchanger may receive a first fluid flow travelling to a suction of the compressor in the HVACR system and second flow of working fluid that is travelling from a heat exchanger receiving the discharge of the compressor to an expansion device. Superheating of the first working fluid may be determined based on temperature measurements prior to and following the suction line heat exchanger. The superheating may be used to control the quantity of the second flow of working fluid introduced into the suction line heat exchanger, for example to maintain superheat that is below a threshold value. These systems may include chillers and heat pump systems, and methods may be applied to chillers or heat pump systems.

Abstract: Systems and methods for controlled subcooling of working fluid in a heating, ventilation, air conditioning and refrigeration (HVACR) system through a suction line heat exchanger are disclosed. The suction line heat exchanger may receive a first fluid flow travelling to a suction of the compressor in the HVACR system and second flow of working fluid that is travelling from a heat exchanger receiving the discharge of the compressor to an expansion device. Superheating of the first working fluid may be determined based on temperature measurements prior to and following the suction line heat exchanger. The superheating may be used to control the quantity of the second flow of working fluid introduced into the suction line heat exchanger, for example to maintain superheat that is below a threshold value. These systems may include chillers and heat pump systems, and methods may be applied to chillers or heat pump systems.

Abstract: There is disclosed a reversible heat pump system 100 and a method of operating a reversible heat pump system to control the temperature of a process fluid of a chiller system 500. In a cooling mode, a working fluid is circulated for co-current flow with a process fluid at a heat exchanger 104 functioning as an evaporator heat exchanger, whereas in a heating mode, the working fluid is circulated for counter-current flow with the process fluid at the same heat exchanger 104 functioning as a condenser heat exchanger.

Abstract: Systems and methods for controlled subcooling of working fluid in a heating, ventilation, air conditioning and refrigeration (HVACR) system through a suction line heat exchanger are disclosed. The suction line heat exchanger may receive a first fluid flow travelling to a suction of the compressor in the HVACR system and second flow of working fluid that is travelling from a heat exchanger receiving the discharge of the compressor to an expansion device. Superheating of the first working fluid may be determined based on temperature measurements prior to and following the suction line heat exchanger. The superheating may be used to control the quantity of the second flow of working fluid introduced into the suction line heat exchanger, for example to maintain superheat that is below a threshold value. These systems may include chillers and heat pump systems, and methods may be applied to chillers or heat pump systems.

Abstract: Systems and methods for controlled subcooling of working fluid in a heating, ventilation, air conditioning and refrigeration (HVACR) system through a suction line heat exchanger are disclosed. The suction line heat exchanger may receive a first fluid flow travelling to a suction of the compressor in the HVACR system and second flow of working fluid that is travelling from a heat exchanger receiving the discharge of the compressor to an expansion device. Superheating of the first working fluid may be determined based on temperature measurements prior to and following the suction line heat exchanger. The superheating may be used to control the quantity of the second flow of working fluid introduced into the suction line heat exchanger, for example to maintain superheat that is below a threshold value. These systems may include chillers and heat pump systems, and methods may be applied to chillers or heat pump systems.

Abstract: Systems and methods for controlled subcooling of working fluid in a heating, ventilation, air conditioning and refrigeration (HVACR) system through a suction line heat exchanger are disclosed. The suction line heat exchanger may receive a first fluid flow travelling to a suction of the compressor in the HVACR system and second flow of working fluid that is travelling from a heat exchanger receiving the discharge of the compressor to an expansion device. Superheating of the first working fluid may be determined based on temperature measurements prior to and following the suction line heat exchanger. The superheating may be used to control the quantity of the second flow of working fluid introduced into the suction line heat exchanger, for example to maintain superheat that is below a threshold value. These systems may include chillers and heat pump systems, and methods may be applied to chillers or heat pump systems.