Abstract: A microelectrode sensor for detecting Staphylococcus aureus and a preparation method and an application method thereof are provided. The microelectrode sensor is a three-electrode system sensor with a platinum wire electrode as a counter electrode and an Ag/AgCl electrode as a reference electrode, and a working electrode is a silver microelectrode modified by chitosan, catechol and Staphylococcus aureus aptamers. The microelectrode sensor is constructed based on the microelectrode with micro/nano structure on the surface, dual media (Fc-Ru3+), chitosan, and catechol. The working electrode can specifically capture Staphylococcus aureus. During the CV test of the microelectrode sensor, the Cl? in bacteria will be leaked to a surface of the working electrode under the action of local high-field strength, and when Cl? ions exists, the cathodic reduction peak of the working electrode can be specifically enhanced between 0 to ?0.1 V, the goal of specific detection of bacteria can be achieved.

Abstract: A microelectrode sensor for detecting Staphylococcus aureus and a preparation method and an application method thereof are provided. The microelectrode sensor is a three-electrode system sensor with a platinum wire electrode as a counter electrode and an Ag/AgCl electrode as a reference electrode, and a working electrode is a silver microelectrode modified by chitosan, catechol and Staphylococcus aureus aptamers. The microelectrode sensor is constructed based on the microelectrode with micro/nano structure on the surface, dual media (Fc-Ru3+), chitosan, and catechol. The working electrode can specifically capture Staphylococcus aureus. During the CV test of the microelectrode sensor, the Cl? in bacteria will be leaked to a surface of the working electrode under the action of local high-field strength, and when Cl? ions exists, the cathodic reduction peak of the working electrode can be specifically enhanced between 0 to ?0.1 V, the goal of specific detection of bacteria can be achieved.