Abstract: The present invention belongs to the field of nanomedicine, and provides a DC biomimetic membrane nanoparticle loaded with an NIR-II AIE dye, a preparation method therefor and use thereof. The DC biomimetic membrane nanoparticle loaded with the NIR-II AIE dye comprises a DC membrane and an NIR-II AIE dye loaded therein, wherein the NIR-II AIE dye has photothermal characteristics. The DC biomimetic membrane nanoparticle loaded with the NIR-II AIE dye provided by the present invention not only solves the problem that the NIR-II AIE dye is poor in water solubility and limited in fluorescence imaging, but also increases the enrichment efficiency of the NIR-II AIE dye coated by the DC membrane at a tumor site and successfully activates the activity of T cells in vivo.

Abstract: Disclosed by the present application are a structure of a double-stranded oligonucleotide nucleic acid probe, a method of use and applications thereof in nucleic acid fluorescence qualitative and quantitative analysis, medical diagnosis and life science researches. The double-stranded oligonucleotide nucleic acid probe is composed of two completely or partially base-complementary oligonucleotide strands; the end of each oligonucleotide strand may be connected to a fluorescent group or a corresponding fluorescent quenching group; and the two oligonucleotide probe strands may hybridize with a target nucleic acid sequence to be tested.