Abstract: A high-throughput optical sectioning three-dimensional imaging system which includes: a light beam modulation module configured to modulate a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens; an imaging module configured to employ a camera to image, in different rows of pixels, a sample under illumination of the modulated light beam; a cutting module configured to cut off an imaged surface layer of the sample; a demodulation module configured to demodulate a sample image of one sample strip of one surface layer into an optical sectioning image, and reconstruct the optical sectioning image of each sample strip of each surface layer into a three-dimensional image. The present disclosure achieves imaging of a whole sample by dividing the sample into at least one surface layer, dividing the at least one surface layer into at least one sample strip, and imaging each sample strip.

Abstract: A high-throughput optical sectioning imaging method and imaging system. The method includes: modulating a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens, the modulated light beam having incompletely identical modulated intensities on the focal plane of the objective lens; imaging, in different rows of pixels, a sample under illumination of the modulated light beam to obtain sample images in the different rows of pixels; obtaining focal plane images of sample images in the different rows of pixels by demodulation of the sample images according to a demodulation algorithm. The system includes a light beam modulation module, an imaging module and a demodulation module.

Abstract: A high-throughput optical sectioning three-dimensional imaging system which includes: a light beam modulation module configured to modulate a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens; an imaging module configured to employ a camera to image, in different rows of pixels, a sample under illumination of the modulated light beam; a cutting module configured to cut off an imaged surface layer of the sample; a demodulation module configured to demodulate a sample image of one sample strip of one surface layer into an optical sectioning image, and reconstruct the optical sectioning image of each sample strip of each surface layer into a three-dimensional image. The present disclosure achieves imaging of a whole sample by dividing the sample into at least one surface layer, dividing the at least one surface layer into at least one sample strip, and imaging each sample strip.

Abstract: A high-throughput optical sectioning imaging method and imaging system. The method includes: modulating a light beam into a modulated light beam capable of being focused on a focal plane of an objective lens and being defocused on a defocusing plane of the objective lens, the modulated light beam having incompletely identical modulated intensities on the focal plane of the objective lens; imaging, in different rows of pixels, a sample under illumination of the modulated light beam to obtain sample images in the different rows of pixels; obtaining focal plane images of sample images in the different rows of pixels by demodulation of the sample images according to a demodulation algorithm. The system includes a light beam modulation module, an imaging module and a demodulation module.

Abstract: A method for automatically altering an imaging area in microscopic imaging of a biological sample. In the method, a sample outline is differentiated from surrounding tissues by means of endogenous or exogenous markers; an initial sample imaging area is set; optical microscopic imaging is performed on a sample surface layer, wherein the imaging area is larger than an area to be imaged of the sample; an actual sample area is calculated by an outline identification algorithm using an imaging result of the sample surface layer and is set as an imaging area of next layer; optical microscopic imaging is performed on a sample to be imaged of the next layer according to the set imaging area, wherein the imaging area covers the area to be imaged of the sample and no redundant imaging is performed; and the above steps are repeated until a data acquisition task is completed.

Abstract: A method for automatically altering an imaging area in microscopic imaging of a biological sample. In the method, a sample outline is differentiated from surrounding tissues by means of endogenous or exogenous markers; an initial sample imaging area is set; optical microscopic imaging is performed on a sample surface layer, wherein the imaging area is larger than an area to be imaged of the sample; an actual sample area is calculated by an outline identification algorithm using an imaging result of the sample surface layer and is set as an imaging area of next layer; optical microscopic imaging is performed on a sample to be imaged of the next layer according to the set imaging area, wherein the imaging area covers the area to be imaged of the sample and no redundant imaging is performed; and the above steps are repeated until a data acquisition task is completed.