Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: An optical module and an optical system thereof for head-up display, and a vehicle, relating to the technical field of head-up display. The optical module includes an image generation element and a light blocking assembly which are provided in sequence; the image generation element is used for forming an image signal, the image signal being incident on a receiving end through the light blocking assembly; and the light blocking assembly is further used for transmitting part of light in external light according to a polarization direction and/or wavelength. The image signal is not affected by the light blocking assembly when passing through the light blocking assembly; the external light passes through the light blocking assembly and the image generation element in sequence, and does not generate an image after passing through the image generation element; light heat of the external light would be gathered on the image generation element.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: A duplex optical connector includes a holder, a pair of optical connectors rotatably disposed in the holder, a cable boot slidably attached to the holder, and a clip removably attached to the holder. The clip functions to maintain a lateral position of the optical connectors relative to the holder and transfer applied force on the pull boot into actuating force on the connector latches. Connector openings formed in the holder are elongated in the lateral direction to provide separate use and polarity reversal connector positions, wherein the connectors non-rotatably engage the holder in their use position and rotatably engage the holder in their polarity reversal position.

Abstract: A duplex optical connector includes a holder, a pair of optical connectors rotatably disposed in the holder, a cable boot slidably attached to the holder, and a clip removably attached to the holder. The clip functions to maintain a lateral position of the optical connectors relative to the holder and transfer applied force on the pull boot into actuating force on the connector latches. Connector openings formed in the holder are elongated in the lateral direction to provide separate use and polarity reversal connector positions, wherein the connectors non-rotatably engage the holder in their use position and rotatably engage the holder in their polarity reversal position.

Abstract: A multi-fiber push-on/pull-off (MPO) connector and an MPO connector tool configured to facilitate insertion and removal into and from an MPO adapter. The MPO connector includes a ferrule, an inner housing holding the ferrule, an outer housing slidably coupled to the inner housing, and a clip coupled to one end of the outer housing and including a main body, a pair of longitudinally extending latch arms for engaging the outer housing, and a pair of laterally extending posts extending from the main body. In use, the MPO connector tool is positionable to apply a force on the laterally extending posts to translate the clip thereby translating the outer housing longitudinally to facilitate connector insertion and removal.

Abstract: A multi-fiber push-on/pull-off (MPO) connector and an MPO connector tool configured to facilitate insertion and removal into and from an MPO adapter. The MPO connector includes a ferrule, an inner housing holding the ferrule, an outer housing slidably coupled to the inner housing, and a clip coupled to one end of the outer housing and including a main body, a pair of longitudinally extending latch arms for engaging the outer housing, and a pair of laterally extending posts extending from the main body. In use, the MPO connector tool is positionable to apply a force on the laterally extending posts to translate the clip thereby translating the outer housing longitudinally to facilitate connector insertion and removal.

Abstract: Disclosed herein are minimal arrestin domain containing protein 1 (ARRDC1) constructs, which drive the formation of ARRDC1-mediated microvesicles (ARMMs). These vesicles can be harnessed to package and deliver a variety of molecular cargos such as small molecules, nucleic acids, and proteins. An example of such cargo is the genome editor Cas9.

Abstract: The present disclosure provides assemblies for supporting optical fiber connections. In embodiments, an optical fiber box includes a main body and a cover defining an installation cavity. A separator and a plurality of spacers are arranged in the main body and divide the installation cavity into first and second cavities and a plurality of installation channels for receiving optical fiber connectors of a type including a sleeve. Installation holes arranged at intervals along the length of the separator receive the sleeves. The plurality of installation channels and the plurality of installation holes are arranged in one-to-one correspondence. The optical fiber box optionally includes a shutter assembly at the entrance to each installation channel. In embodiments, at least the main body, the separator, and the plurality of spacers are integrally formed to provide a connection solution that does not require separate optical fiber adapters.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: The present disclosure provides assemblies for supporting optical fiber connections. In embodiments, an optical fiber box includes a main body and a cover defining an installation cavity. A separator and a plurality of spacers are arranged in the main body and divide the installation cavity into first and second cavities and a plurality of installation channels for receiving optical fiber connectors of a type including a sleeve. Installation holes arranged at intervals along the length of the separator receive the sleeves. The plurality of installation channels and the plurality of installation holes are arranged in one-to-one correspondence. The optical fiber box optionally includes a shutter assembly at the entrance to each installation channel. In embodiments, at least the main body, the separator, and the plurality of spacers are integrally formed to provide a connection solution that does not require separate optical fiber adapters.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of cargo proteins into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of cargo proteins, such as transcription factors, tumor suppressors, developmental regulators, growth factors, metastasis suppressors, pro-apoptotic proteins, nucleases, recombinases, and reprogramming factors into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.

Abstract: Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of Cas9 proteins and/or Cas9 variants into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.