Abstract: A sample pretreatment kit for detecting virus infectivity includes a photoactivatable dye capable of intercalating into a nucleic acid, and a nuclease capable of degrading the nucleic acid. The photoactivatable dye includes PMA dye, PMAxx dye, EMA, platinum compounds, or palladium compounds. The method for detecting virus infectivity includes steps of: (a) dividing a clinical sample into a test sample and a control sample; (b) treating the test sample with a photoactivatable dye and a nuclease; (c) exposing the test sample to a light for photoactivation; (d) amplifying a target nucleic acid in the test sample and the control sample; and (e) determining virus infectivity based on amplification results of the test sample and the control sample.

Abstract: A sample collection device includes a tube, a closure and a partitioning member. The tube includes an opening and is used to receive a sampling swab. The closure is engaged with the tube for enclosing the opening. The partitioning member is disposed in the tube and includes a blocking portion and a position-limiting portion. The blocking portion is disposed close to the opening and covers a portion of the opening so as to leave another uncovered portion as an entrance for passing the sampling swab therethrough. The position-limiting portion is connected with the blocking portion and extended into the tube, so as to limit the sampling swab in a space corresponding to the entrance inside the tube after the sampling swab passes through the entrance.

Abstract: A method for detecting infectivity of a human coronavirus is provided. The method includes steps of: (a) dividing a testing sample into a first sample and a second sample; (b) treating the first sample with an intercalating dye or chemical; (c) exposing the first sample to a light for photo-activation; (d) amplifying targeted nucleic acids in the first sample and the second sample; and (e) determining infectivity of the human coronavirus based on amplification results of the first sample and the second sample.