Abstract: The present invention discloses a PIS genotype detection method for goats and belongs to field of life science. The method includes 4 pairs of specific primers: SRY F and SRY R; PIS wt F and PIS wt R; PIS var-1 F and PIS var-1 R; and PIS var-2 F and PIS var-2 R, and a probe. The present invention further provides a specific detection method and a detection standard. A simple and efficient detection method for diagnosing a PIS genotype of the goats is developed in combination with a high-sensitivity PCR method and a high-stability Southern Blot method, has extremely high specificity, is convenient and rapid, and may be used for accurately identifying genotypes of PIS genes, guiding breeding and mating of the goats and controlling or adjusting genotype frequencies of the PIS genes in goat populations according to requirements of breeders, to increase economic benefits of goat farming.