Abstract: The present disclosure relates to aflibercept, in particular, attributes of aflibercept. Also provided herein are methods of characterizing and modifying the attributes of aflibercept and compositions comprising aflibercept with particular attributes.

Abstract: Disclosed herein are methods for harvesting a recombinant protein comprising an asparaginyl endopeptidase (AEP) cleavage site, as well as methods for inhibiting cleavage of a recombinant protein comprising an AEP cleavage site, during a biomanufacturing process.

Abstract: The present disclosure relates to aflibercept, in particular, attributes of aflibercept. Also provided herein are methods of characterizing and modifying the attributes of aflibercept and compositions comprising aflibercept with particular attributes.

Abstract: Electrospray ionization (ESI) apparatuses and methods of making the same are disclosed. In one embodiment, an ESI emitter comprises a capillary tube filled with a porous monolithic material, wherein the end of the emitter is tapered and the tip of the tapered end comprises a protrusion of the porous monolithic material. One embodiment of the method for making ESI emitters comprises filling a capillary tube with a porous monolithic material and tapering the end of the capillary tube, thereby exposing a portion of the porous material. The exposed portion of the porous material at the tapered end, rather than the capillary tube itself, forms the tip of the ESI emitter.

Abstract: The use of porous monolithic matrix for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis on a sample suspected of containing an analyte is herein described. The porous polymer monoliths provide a matrix suitable for use in detecting and analyzing low molecular weight and small molecules. The matrices described herein also facilitate the creation of arrays of monolithic matrices for high-throughput detection and screening using MALDI-TOF mass spectrometry and exhibit a long shelf-life.