Abstract: The disclosure relates to a preparation and purification method for an antibody drug conjugate intermediate, and more particularly relates to a preparation and purification method for a linker part and drug part conjugate in an antibody drug conjugate, which can not only effectively remove impurities from a target product and by-products in a reaction process, making the purity of the target product finally obtained up to 99% or above, but also realize stable mass production and well meet the quality standard requirements of clinical drugs, so as to provide a tremendous guarantee for drug safety and stable supply.