Abstract: A method of determining the quantity of two or more species in a polydisperse sample is provided. The method comprising the steps of: performing a series of dilution steps of the polydisperse sample; adding a probe into the polydisperse sample, wherein the probe is configured to bind to two or more species to form two or more probe-target species complexes; separating two or more probe-target species complexes at each dilution step into two or more bins; determining the concentration of each separated probe-target species complex in each bin at each dilution step; and determining the quantity of each target species in the sample and/or the affinity of each target species to the probe.

Abstract: A method of determining diffusion coefficient of one or more components in a polydisperse sample is provided. The method comprising the steps of: introducing an auxiliary fluid flow into a fractionation channel; introducing the polydisperse sample comprising one or more components into the fractionation channel; allowing the sample and the auxiliary fluid to create a combined flow; fractionating the combined flow into two or more fractions by diffusive sizing; subsequently separating two or more components from each fraction by creating a distribution of the components within a separation channel; detecting a characteristic of each the two or more components in each fraction; and comparing the characteristic of each component in each fraction in order to determine the diffusion coefficient of each of the one or more components in the polydisperse sample.

Abstract: Method of tracking a plurality of objects comprising: focusing an image of the objects on an imaging element using an optical system; capturing the image of the objects using an imaging element comprising a plurality of pixels; measuring at least one characteristic of the objects from the captured image using an image processor; wherein the field of view is set be the widest field of view for which the image processor is able to measure the at least one characteristic.