Abstract: Bivalent antibody constructs comprising (a) a first polypeptide chain comprising a VL sequence, a CH3 sequence, a CH2 sequence, and a CH3 sequence, arranged from N- to C-terminus in a VL-CH3-CH2-CH3 orientation; (b) a second polypeptide chain comprising a VH sequence and a CH3 sequence, arranged from N- to C-terminus in a VH-CH3 orientation; (c) a third polypeptide chain comprising a VL sequence, a CL sequence, an antibody CH2 sequence, and a CH3 sequence, arranged from N- to C-terminus in a VL-CL-CH2-CH3 orientation; (d) a fourth polypeptide chain comprising a VH sequence and a CH1 sequence, arranged from N- to C-terminus in a VH-CH1 orientation; wherein the amino acid sequences of the C-terminal CH3 domains in the first and third polypeptide chains are different from one another and comprise complementary knob-in-hole mutations, and pharmaceutical compositions comprising the antibody constructs, and methods of use thereof are provided.

Abstract: Multispecific Treg-binding molecules, constructs, pharmaceutical compositions comprising the constructs, and methods of use thereof are presented.

Abstract: Multispecific Treg-binding molecules, constructs, pharmaceutical compositions comprising the constructs, and methods of use thereof are presented.

Abstract: Multivalent receptor-clustering agonist antibody constructs are provided. The constructs are capable of (i) binding a cell surface receptor target that requires clustering for agonist activity, and (ii) clustering the receptor target on the cell surface in the absence of an independent cross-linking agent. Each of the target receptor-binding antigen binding sites of the construct is contributed by antibody variable region binding domains. Also provided are pharmaceutical compositions comprising the antibody construct, and methods of treating diseases, notably cancer, by administering therapeutically effective amounts of the pharmaceutical composition.

Abstract: Trispecific trivalent antibody constructs, pharmaceutical compositions comprising the constructs, and methods of use thereof are presented.

Abstract: Binding molecules, constructs, pharmaceutical compositions comprising the constructs, and methods of use thereof are presented.

Abstract: Anti-ROR antibody constructs, pharmaceutical compositions comprising the constructs, and methods of use thereof are presented.

Abstract: Multivalent antibody constructs, pharmaceutical compositions comprising the constructs, and methods of use thereof are presented.